2004 Fiscal Year Final Research Report Summary
Scintigraphic imaging of programmed cell death, apoplcssis, in ischemic myocardium
| Project/Area Number |
14570842
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
TAKI Junichi Kanazawa University, Graduate School of Medical Science, Assistant professor, 医学系研究科, 講師 (10251927)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Kenichi Kanazawa University Hospital, Assistant professor, 医学部付属病院, 講師 (00167545)
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| Project Period (FY) |
2002 – 2004
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| Keywords | apoptosis / myocardial ischemia / myocardial infarction / ischemic precondition / caspase inhibitor / reperfusion / necrosis |
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| Research Abstract |
To determine the time course, location and extent of this process in various severity of ischemia we studied ^<99m>Tc-annexin-V uptake in groups of rats following various intervals of coronary occlusion (20, 15, 10, 5 min) and reperfusion time (0.5, 1.5, 6, 24 hr, 3 day, 14 day). After reperfusion ^<99m>Tc-annexin-V was injected and 1 h later the rats were sacrificed. Dual tracer autoradiography was performed to assess ^<99m>Tc-annexin-V uptake and area at risk. In all 5-min occlusion and reperfusion models, no significant ^<99m>Tc-annexin V uptake was observed in the area at risk. In 10-min, 15-min, and 20-min ischemia and reperfusion models, significant ^<99m>Tc-annexin V uptake was observed depending on the severity of ischemia and reperfusion time. In each group of rats with the same reperfusion time, ^<99m>Tc-annexin V uptake depended on the severity of ischemia ; higher uptake in longer occlusion time. In each group of rats with the same ischemic time, ^<99m>Tc-annexin V uptake was highest in 0.5 h reperfusion model and the uptake decreased time dependently. In 20-min ischemia, a certain level of histological evidence of infarction was observed. In 15, 10, 5-min ischemia, there was no significant histological change was observed in 10-min and 5-min ischemia group. These data indicate that annexin binding depends on the severity of ischemia which results in the wide spectrum of histological change ; that is from infarction to no significant histological change. ^<99m>Tc-annexin V imaging may be feasible in the assessment of acute myocardial ischemia without infarction as well as infarction.
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Research Products
(12 results)
