2003 Fiscal Year Final Research Report Summary
FOR CLINICAL APPLICATION OF PANCREATIC ISLET TRANSPLANTATION-ANALYSIS OF THE PANCREATIC ISLETS GROWTH FACTOR USED MICRODISSECTION
| Project/Area Number |
14571116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | FUKUOKA UNIVERSITY |
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Principal Investigator |
ANZAI Keizo Fukuoka University, Hospital, Assistant Professor, 病院, 講師 (60258556)
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| Co-Investigator(Kenkyū-buntansha) |
IRIE Taro Shouwa Univ., School of Dentistry, Assistant Professor, 歯学部, 講師 (00317570)
TACHIKAWA Tetsuhiko Shouwa Univ., School of Dentistry, Professor, 歯学部, 教授 (10085772)
YASUNAMI Yoichi Fukuoka Univ., School of Medicine, Assoc. Prof., 医学部, 助教授 (00166521)
KOGAWA Kazuhiko Fukuoka Univ., Hospital, Instructor, 病院・助手 (60360301)
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| Project Period (FY) |
2002 – 2003
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| Keywords | PANCREATIC ISLET TRANSPLANTATION / TYPE 1 DIABETES MELLITUS / PROLIFERATING GENE OF PANCREATIC ISLET |
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| Research Abstract |
At present, Islet transplantation is highlighted as one alternative of treatment for type 1 diabetes mellitus. In this study, we analyze the gene expression in the proliferating islets and intend to identify the main factors that cause the islet cells to proliferate, keeping insulin secretional capacity and glucose responsibility. We used the partial pancreatectomy mice as in vivo proliferation model of islets. One important advantage of this study is that we used the islets which proliferated and regenerated, keeping insulin secretional capacity and glucose responsibility in the physiological environment in vivo. Another is that we selectively recovered the proliferating islets using Laser Captured Microdissection (LCM). Using LCM, we can selectively, recover the part of the purpose from the tissue section affixed on the slide glass. by the laser. Actually, we selectively cut off the islets which proliferated in the partial pancreatectomy mice using the LCM, and extracted the total RNA, and then analyzed the expressing genes by the cDNA microarray. Reflecting the functional acceleration of islets in partial pancreatectomy mice, the gene expression such as glucagon were increased. At present, we are analyzing how these genes are concerned in the proliferation of islet.
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