Antioxidant Agents Therapy for Esophageal Mucosa with Inducible Nitric Oxide Syntlaase Expression

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 14571230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Tokai University
• Principal Investigator: TANAKA Hikaru Tokai University, School of medicine, Assistant Researcher, 医学部, 助手 (20266414)
• Co-Investigator(Kenkyū-buntansha): KIJIMA Hiroshi Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (90204859)
• Project Period (FY): 2002 – 2003
• Keywords: esophageal carcinoma / rat / iNOS / anthocyanins / curemin / sesamin

Research Abstract

2002 : We made the antioxidation feed which mixed blueberry (anthocyanins)10% sesame (sesamin) 10%, turmeric (curumin)10% with each. It was given the group of treatments rat N-Methyl-N-anyhtitrosamine (following AMN) since administered antioxidation feed for one week. The crowd whom administered antioxidation feed and AMN to and the control crowd whom administered normal feed and ANN to administered it with 12 weeks for ten weeks for each eight weeks, and sacrifice was made to die. The appearance of iNOS was accepted in eight weeks, but cancer-Causing frequency was held down. For ten weeks, carcinogenesis was accepted in 12 weeks by all rats. As the intake situation of antioxidation feed, some feed with turmeric bit it and remained in a bottom of a gauge in large quantities, and it was thought that there were not most of the effects of turmeric, but when continued administering a cancer-causing agent together, a carcinogenesis prevention effect by quality of these antioxides might dela y carcinogenesis temporarily, but that the effect that completely controlled carcinogenesis was not provided was supposed.
2003 : We analyzed a study result of 2002 again. If we did carcinogenesis entirely in eight weeks and bred it by the dosage for eight weeks in conventional AMN afterwards until 12 weeks, careinogenesis of 100% was the provided situation and, with a rat of control, was different from the time that the cancer-causing time by AMN thought about at first greatly. Because AMN which we used conventionally purchased AMN from an other chemical reagent company because release was called off, it is thought that the cancer-causing time changed by a difference of purity of AMN. A rat carcinogenesis experiment by AMN was cancer-causing experiment system developed in this institution and there was not a document and added enough examination besides conventional accumulation data about the dosage density and a dosage period of AMN. Now do cancer-causing fundamental experiment between the dosage period of AMN for six weeks for five weeks for four weeks for two weeks.1 set the result between the dosage period for the cause again and am to administer AMN to a special feed dosage rat.