Establishment of experimentally reflux esophagitis after distal partial gastrectomy in rats and pathophysiological investigation.

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 14571233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Jikei University School of Medicine
• Principal Investigator: OMURA Nobuo Jikei University School of Medicine, Lecturer, 医学部, 講師 (70271301)
• Co-Investigator(Kenkyū-buntansha): TSUBOI Kazuto Jikei University School of Medicine, Research Assistant, 医学部, 助手 (20338924)
• Project Period (FY): 2002 – 2003
• Keywords: reflux esophagitis / distal partial gastrectomy / duodenal juice / rat / bile / pancreatic juice

Research Abstract

【Background】 Duodenal juice and acid in the remnant stomach would play important roll in reflux esophagitis after distal partial gastrectomy. We could make experimentally reflux esophagits in rats with anterectomy and gastroduododenal anastomosis (Biliroth I method). However, occurrence ratio of esophagitis and survival ratio were low (report in 2002). We retried to make some model for reflux esophagitis after gastrectomy. 【Materials and method】 We used five group of rats. Group 1 ; the control group, Group 2 ; gastroduodenal anastomosis after antrectomy, Group 3 ; covered with a thin catheter around duodenum at distal side of papilla vater in group 2, Group 4 ; ligation of limiting ridge (between the glandular portion and the forestomcb) in group 2, Group 5 ; ligation of limiting ridge in group 3. The animals were sacrifled 2 weeks after the start of experiment. We investigated the lesion of the esophagus macroscopically and histologically and measured remnant gastric pH. 【Results】 (1) Survival ratio : 100% in group 1, 50% in group 2, 50% in group 3, 40% in group 4, 40% in group 5, respectively. (2) Occurrence ratio of esophagitis 0% in group 1, 40% in group 2, 60% in group 3, 75% in group 4, 100% in group 5. (3) Histological examination : Marked thickening of the esophageal epitheliuxn, the elongation of papillae of the lainina propria into the epithelium and infiltration of inflammatory cells in the lamina propria were remarkable in the rats with esophagitis. 【Conclusion】 Ligation of limiting ridge was an important point in experimentally reflux esophagitis after distal gastrectomy.