2003 Fiscal Year Final Research Report Summary
Interleukin 6 Production and Tumor Proliferation in Lung Cancer.
| Project/Area Number |
14571256
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
IIZASA Toshihiko Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (10272303)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OTSUJI Mizuto Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (50344982)
SHIBUYA Kiyoshi Chiba University, University Hospital, Lecturer, 医学部附属病院, 講師 (20302565)
FUJISAWA Takehiko Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (80110328)
IYODA Akira Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (10302548)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Keywords | Non-Small Cell Lung Cancer / Interleukin 6 / Immunotherapy / Gene Therapy / Microarray |
|---|
| Research Abstract |
Interleukin 6 (IL-6) facilitates the differentiation of B cells to immunoglobulin secreting cells and is reported to be a proliferative factor in some tumors. In this study, we examined IL-6 production in non-small cell carcinoma (NSCLC) and the proliferation of tumor cells following IL-6 treatment in vitro and in vivo. We analyzed the expression of IL-6 mRNA and protein in a series of 15 human lung cancer cell lines (4 adenocarcinomas, 5 squamous call carcinomas, 2 large cell carcinomas and 4 small cell carcinomas) by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). We established an IL-6 producing cell line (ABC-1 #IL-6) by transfecting a human IL-6 cDNA into a human IL-6 non-producing NSCLC cell line (ABC-1). These two cell lines were used to determine tumor cell proliferation both in vivo and in vitro in order to clarify the effect of IL-6 on tumor growth and metastasis. Athymic nude mice, SCID mice and BALB/c mice were subcuta
… More
neously inoculated with these two cell lines and body weight, tumor growth and tumor doubling time were measured. The presence of IL-6 and tumor infiltrating lymphocytes (TILs) within tumor tissues was examined by immunohistochemical staining. Results: Eight of 15 (53%) lung cancer cell lines expressed both IL-6 mRNA and protein. Tumor lesions of both cell lines developed in nude and SCID mice although no such lesions of either cell lines developed in BALB/c mice. The tumor doubling time in nude and SCID mice was 2.97+/-1.22 days and 2.45+/-1.32 days respectively in mice inoculated with the cell line ABC-1#IL-6. These doubling times were statistically significantly shorter than those evident in mice inoculated. with the control original ABC-1 cell line (nude : p=0.0337, SCID : p=0.0119, unpaired t-test). The rates of cell proliferation in vitro of the ABC-1#IL-6 and original ABC-1 cells lines were comparable (p=0.1441 / unpaired t-test). Immunohistochemical staining revealed strong IL-6 expression in tumors derived from the IL-6 producing cell line but not in tumors derived from the original ABC-1 cell line (both in nude and SCID mice). Conclusion: 53% of lung cancer cell lines produce IL-6 mRNA and protein. Although IL-6 itself does not influence tumor cell proliferation in vitro, an association between IL-6 expression and tumor proliferation was found in vivo in nude and SCID mice. An anti-IL-6 reagent could provide a novel therapeutic strategy in patients with IL-6-producing lung tumors. Less
|
Research Products
(2 results)
-
[Publications] Yamaji H, Iizasa T, Koh E, Suzuki M, Otsuji M, Chang H, Motohashi S, Yokoi S, Hiroshima K, Tagawa M, Nakayama T, Fujisawa T.: "Correlation between Interleukin 6 Production and Tumor Proliferation in Non-Small Cell Lung Cancer."Cancer Immunol Immunother. (in press). (2004)
Description
「研究成果報告書概要(欧文)」より
-
