2003 Fiscal Year Final Research Report Summary
Regenerative capacity of decellularized valvular grafts after transplantation
| Project/Area Number |
14571274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | NARA MEDICAL UNIVERSITY |
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Principal Investigator |
NAGASAKA Shigeo Nara Medical University, Surgery III, Research Associate, 第三外科, 助手 (20347553)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Takehisa Nara Medical University, Surgery III, Research Associate, 第三外科, 助手 (20305717)
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| Project Period (FY) |
2002 – 2003
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| Keywords | heart valve transplantation / decellularization / tissue regeneration / cryopreservation |
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| Research Abstract |
1.In vivo study of the effects of cryopreservation on heart valve xenotransplantation
We investigated in vivo morphologic changes of cryopreserved xenogeneic heart valves in compalison with fresh xeno or cryopreserved allograft valves. There was much cellular infiltration in cryopreserved xenograft valves in one month after transplantation. However, the explanted fresh xenograft valves did not have any cellurality itself. Immunomodulation with cryopreservation might operate on the morphological changes in heart valve xenotransplantation.
2.Evaluation of cryopreserved cardioyascular allograft viability using real-time quantitative PCR
We quantitatively investigated the viability in cryopreserved allograft artery by measuring mRNA levels of VEGF,HGF,FGF, and Reg using the real-time RT-PCR method. After cryopreservation, the cell viability was about 70% compared with fresh aorta. This was severely-impacted by the immune response and immunosuppressant. We concluded that the quantification of mRNAs using real-time PCR was acceptable method for evaluation of graft viability.
3.Relationship between immunological rejection and matrix Gla protein in cryopreserved vascular allografts
Recent attention has focused on the inhibitory effect of matrix Gla protein (MGP) on ectopic vascular calcification, but the behavior of MGP in cryopreserved allografts is uncertain. We examined the relationship between immunological rejection and MGP in cryopreserved rat aortic grafts after transplantation. The immunological rejection is likely to inhibit MGP expression in cryopreserved vascular allografts, resulting in late-onset calcification.
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