2003 Fiscal Year Annual Research Report

子宮内膜症の進展および子宮筋腫の発症とダイオキシン受容体関連蛋白多型との相関

Research Project

Project/Area Number	14571582
Research Institution	KEIO UNIVERSITY
Principal Investigator	浅田弘法慶應義塾大学, 医学部, 助手 (60231883)
Co-Investigator(Kenkyū-buntansha)	吉村泰典慶應義塾大学, 医学部, 教授 (10129736) 小崎健次郎慶應義塾大学, 医学部, 講師 (30234743)
Keywords	子宮内膜症 / 子宮筋腫
Research Abstract	われわれは、内分泌攪乱物質であるダイオキシンに注目して、ダイオキシン関連遺伝子群の検出とその遺伝子多型の解析を行ってきた。すなわち、ダイオキシン受容体関連遺伝子であるダイオキシン受容体(AHR)、ダイオキシン受容体核内転位因子1(ARNT1)、ダイオキシン受容体核内転位因子2(ARNT2)、ダイオキシン受容体抑制因子(AHRR)および、アンドロゲン受容体の遺伝子多型の検出を行った。また、われわれは遺伝子データベースを用いた解析により、一定の遺伝子に関連して変動する遺伝子群を検索する方法を開発してきたが、この方法を、ダイオキシンレスポンシブエレメントに応用し、ダイオキシンレスポンシブエレメントが関連している遺伝子群のスクリーニングを行い、ダイオキシン関連候補遺伝子を検出するとともにその候補遺伝子の遺伝子多型の解析も同時に進めた。また、腹腔鏡手術を中心とした手術所見および病理所見により子宮内膜症および子宮筋腫と確実に診断がなされた患者から、同意を得た上で検体を集積してきた。子宮内膜症患者、および子宮筋腫患者の末梢血よりDNAを抽出し、AHR、AHRR、ARNT1、ARNT2および新たに検出したダイオキシン関連遺伝子の遺伝子多型解析について解析を行った。今回検討した症例においては、子宮内膜症の発症、および子宮筋腫の発症に特異的な単一遺伝子の遺伝子多型部位はAHR、AHRR、ARNT1、ARNT2においては検出されなかった。

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Maruyama T, Yamamoto Y, Shimizu A, Masuda H, Sakai N, Sakurai R, Asada H.Yoshimura Y: "Pyrazolo pyrimidine-type inhibitors of src family tyrosine kinases promote ovarian steroid-induced differentiation of human endometrial stromal cells In Vitro."Biol Reprod. 70(1). 214-221 (2004)
[Publications] Sakai N, Maruyama T, Sakurai R, Masuda H, Yamamoto Y, Shimizu A, Kishi I, Asada H, Yamagoe S, Yoshimura Y: "Involvement of histone acetylation in ovarian steroid-induced decidualization of human endometrial stromal cells."J Biol Chem. 278(19). 16675-16682 (2003)
[Publications] Shimasaki N, Watanabe K, Hara M, Kosaki K: "EYA1 Mutation in a Newborn Female Presenting with Cardiofacial Syndrome."Pediatr Cardiol.. Dec18. (2003)
[Publications] Jones KL, Kosaki K, Hall BD: "X-linked cubitus valgus with mental retardation and typical face."Am J Med Genet.. 123A(1). 33-36 (2003)
[Publications] Ogata T, Sato S, Hasegawa Y Kosaki K: "Lymphstasis in a boy with Noonan syndrome : implication for the development of skeletal features."Endocr J.. 50(3). 319-324 (2003)
[Publications] Sasaki G, Ogata T, Ishii T, Kosaki K, Sato S, Homma K, Takahashi T, Hasegawa T, Matsuo N: "Micropenis and the 5alpha-reductase-2 (SRD5A2) gene : mutation and V89L polymorphism analysis in 81 Japanese patients."J Clin Endocrinol Metab.. 88(7). 3431-3436 (2003)
[Publications] Akahoshi K, Sakazume S, Kosaki K Ohashi H, Fukushima Y: "EEC syndrome type 3 with a heterozygous germline mutation in the P63 gene and B cell lymphoma."Am J Med Genet.. 120A(3). 370-373 (2003)
[Publications] Matsumoto T, Miyakoshi K, Kasai K, Ishimoto H, Tanaka M, Ikeda K, Yoshimura Y: "Fetal goitrous hypothyroidism followed by neonatal transient hypoerthyroidism."Fetal Diagn Ther.. 18. 459-462 (2003)
[Publications] Yoshimura Y: "Oocyte maturation in humans : the potential relevance to reproductive medicine."J Mamm Ova Res.. 20. 86-92 (2003)
[Publications] Kashiwagi S, Miyakoshi K, Ishimoto H, Tanaka M, Yoshimura Y: "Checkmark fetal heart rate pattern associated with severe fetal hypoxia: A case report."Fetal Diagn.Ther.. 18. 237-239 (2003)
[Publications] Iwahashi K, Kuji N, Fujiwara T, Tanaka H, Takahashi J, Inagaki N, Komatsu S, Yamamoto A, Yoshimura Y, Akagawa K: "Expression of the exocytotic protein syntaxin in mouse oocytes."Reproduction.. 126. 73-81 (2003)
[Publications] Miyakoshi K, Tanaka M, Ueno K, Uehara K, Ishimoto H, Yoshimura Y: "Cutoff value of 1 h, 50 g glucose challenge test for screening of gestational diabetes mellitus in a Japanese population."Diabetes Res Clin Pract.. 60. 63-67 (2003)
[Publications] Tanaka Y, Kato S, Tanaka M, Kuji N, Yoshimura Y: "Structure and expression of the human oocyte-specific histone H1 gene elucidated by direct RT-nested PCR of a single oocyte."Biochem Biophys Res Commun.. 304. 351-357 (2003)

2003 Fiscal Year Annual Research Report

子宮内膜症の進展および子宮筋腫の発症とダイオキシン受容体関連蛋白多型との相関

Principal Investigator

浅田 弘法 慶應義塾大学, 医学部, 助手 (60231883)

Research Products

[Publications] Maruyama T, Yamamoto Y, Shimizu A, Masuda H, Sakai N, Sakurai R, Asada H.Yoshimura Y: "Pyrazolo pyrimidine-type inhibitors of src family tyrosine kinases promote ovarian steroid-induced differentiation of human endometrial stromal cells In Vitro."Biol Reprod. 70(1). 214-221 (2004)

[Publications] Sakai N, Maruyama T, Sakurai R, Masuda H, Yamamoto Y, Shimizu A, Kishi I, Asada H, Yamagoe S, Yoshimura Y: "Involvement of histone acetylation in ovarian steroid-induced decidualization of human endometrial stromal cells."J Biol Chem. 278(19). 16675-16682 (2003)

[Publications] Shimasaki N, Watanabe K, Hara M, Kosaki K: "EYA1 Mutation in a Newborn Female Presenting with Cardiofacial Syndrome."Pediatr Cardiol.. Dec18. (2003)

[Publications] Jones KL, Kosaki K, Hall BD: "X-linked cubitus valgus with mental retardation and typical face."Am J Med Genet.. 123A(1). 33-36 (2003)

[Publications] Ogata T, Sato S, Hasegawa Y Kosaki K: "Lymphstasis in a boy with Noonan syndrome : implication for the development of skeletal features."Endocr J.. 50(3). 319-324 (2003)

[Publications] Sasaki G, Ogata T, Ishii T, Kosaki K, Sato S, Homma K, Takahashi T, Hasegawa T, Matsuo N: "Micropenis and the 5alpha-reductase-2 (SRD5A2) gene : mutation and V89L polymorphism analysis in 81 Japanese patients."J Clin Endocrinol Metab.. 88(7). 3431-3436 (2003)

[Publications] Akahoshi K, Sakazume S, Kosaki K Ohashi H, Fukushima Y: "EEC syndrome type 3 with a heterozygous germline mutation in the P63 gene and B cell lymphoma."Am J Med Genet.. 120A(3). 370-373 (2003)

[Publications] Matsumoto T, Miyakoshi K, Kasai K, Ishimoto H, Tanaka M, Ikeda K, Yoshimura Y: "Fetal goitrous hypothyroidism followed by neonatal transient hypoerthyroidism."Fetal Diagn Ther.. 18. 459-462 (2003)

[Publications] Yoshimura Y: "Oocyte maturation in humans : the potential relevance to reproductive medicine."J Mamm Ova Res.. 20. 86-92 (2003)

[Publications] Kashiwagi S, Miyakoshi K, Ishimoto H, Tanaka M, Yoshimura Y: "Checkmark fetal heart rate pattern associated with severe fetal hypoxia: A case report."Fetal Diagn.Ther.. 18. 237-239 (2003)

[Publications] Iwahashi K, Kuji N, Fujiwara T, Tanaka H, Takahashi J, Inagaki N, Komatsu S, Yamamoto A, Yoshimura Y, Akagawa K: "Expression of the exocytotic protein syntaxin in mouse oocytes."Reproduction.. 126. 73-81 (2003)

[Publications] Miyakoshi K, Tanaka M, Ueno K, Uehara K, Ishimoto H, Yoshimura Y: "Cutoff value of 1 h, 50 g glucose challenge test for screening of gestational diabetes mellitus in a Japanese population."Diabetes Res Clin Pract.. 60. 63-67 (2003)

[Publications] Tanaka Y, Kato S, Tanaka M, Kuji N, Yoshimura Y: "Structure and expression of the human oocyte-specific histone H1 gene elucidated by direct RT-nested PCR of a single oocyte."Biochem Biophys Res Commun.. 304. 351-357 (2003)

浅田弘法慶應義塾大学, 医学部, 助手 (60231883)