2003 Fiscal Year Final Research Report Summary
Development of grafting bone tissue from periosteum using in vivo tissue engineering
| Project/Area Number |
14571721
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Teikyo University |
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Principal Investigator |
YONEHARA Yoshiyuki Teikyo University, School of Medicine, Professor, 医学部, 講師 (00251299)
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| Co-Investigator(Kenkyū-buntansha) |
TACHI Masahiro Teikyo University, School of Medicine, Assistant professor, 医学部, 講師 (50312004)
HIRABAYASHI Shinichi Teikyo University, School of Medicine, Professor, 医学部, 教授 (60173259)
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| Project Period (FY) |
2002 – 2003
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| Keywords | perlosteum / bone / bone formation / In vivo / tissue engineering / artificial material / bone reconstruction / collagen |
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| Research Abstract |
Autogenous bone graft still remains the standard for bone reconstructive techniques. However, because of concerns associated with donor site morbidity and because there is only a limited supply of. autogenous bone available, there has been intense interest in the development of bone graft substitutes. The estrogenic capacity of periosteum can also be used to induce bone. Vascularized periosteum has been shown to contribute to osteogenesis in bone development with variable success. The purpose of our study was to observe a newly formed bone fortune by using a vascularized periosteal flap and to evaluate osteogenic capacity of vascularized periosteum with atelo-collagen sponge. Both hind limbs of rats were used to construct vascularized periosteal flaps. A vascularized periosteal flap was dissected off the surface of the tibia and fibula. The flap was tubed on itseW with, the periosteal layer inside. In the atelo-collagen sponge graft case, the tube of right side leg containing atelo-collagen sponge. Bone formation Was evaluated radiographically and histlogically. Good bone formations were radiographically observed in the vascularized periosteal flaps 5 days after operation. Length of newly formed bone gradually increased still 8 weeks after operation. After B weeks operation, newly formed bone length gradually decreased. Width of newly formed bone gradually increased still 4 weeks after operation. After 4 weeks operation, newly formed bone width gradually decreased. On histological evaluation, woven bone formation was observed in the vascularized periosteal flap 5 days after operation. After 2 weeks post operation, lamellar bone formation was observed. New bone formation increased both radiographically and histlogically from 5 days td 4 weeks in the periosteal flaps containing atelo-collage sponge. After 4 weeks operation, there was no significant more bone formation radiographically and histlogicafly in the group with atelo-collagen sponge graft.
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Research Products
(4 results)
