Research of the bone formation promotion effect with used together PRP from self-blood and CCEF.

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 14571855
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: OCHI Morio School of Dentistry, Health Sciences University of Hokkaido, Department of Fixed Prosthodontics, Professor, 歯学部, 教授 (50169322)
• Co-Investigator(Kenkyū-buntansha): MATUBARA Hideki School of Dentistry, Health Sciences University of Hokkaido, Department of Fixed Prosthodontics, Assistant, 歯学部, 助手 (90364257) ; KUNIYASU Hiroya School of Dentistry, Health Sciences University of Hokkaido, Department of Fixed Prosthodontics, Assistant, 歯学部, 助手 (80364256) ; HIROSE Yukito School of Dentistry, Health Sciences University of Hokkaido, Department of Fixed Prosthodontics, Lecturer, 歯学部, 講師 (40244868)
• Project Period (FY): 2002 – 2003
• Keywords: Platelet-rich plasma / Osteogenesis-promot ing effect

Research Abstract

The aim of this study was to carry out a comparative assessment of the osteogenesis-promoting effects of platelet-rich plasma (PRP) in combination with different kinds of carrier material. We created an enclosed space by fixing a cap onto the rabbit parietal bone, in order to study osteogenesis using the guided bone regeneration (GBR) method. The space was filled with PRP along with a bone-compensating carrier to induce osteogenesis, and the amount of osteoneogenesis within the cap was measured.
First, we assessed the various methods for collecting PRP. We then attached a pair of polypropylene caps to the surface of the parietal bone of each rabbit. One cap was filled with one of the following carriers: Collagen, WTCP, thrombin. β-TCP complex, or sodiumalginate (control group); the other cap was filled with therespective carrier soaked with PRP (experimental group). After 8 weeks, undecalcified samples were sectioned and used for histological observations and measurements of the amount of osteoneogenesis. In addition, longitudinal observations of osteogenesis guided by the β-TCP PRP complex were carried out.
The most suitable method for collecting PRP was found to be a double spin method (2400 rpm for 10 mm followed by 3600 rpm for 15 mm), yielding a platelet concentration factor of 13.5. With respect to any of the carriers tested, the amount of osteoneogenesis in the cap was increased in the experimental PRP(+) group compared to the control PRP(-) group. The amount of osteogenesis was greater in the B-TCP PRP complex pair than in any of the other carrier pairs. In the longitudinal study of osteogenesis using the β-TCP PRP complex, PRP was found to increase osteogenesis relatively early in the study period.
Since this experimental study confirms the osteogenesis-promoting effects of the β-TCP ・ PRP complex without the combined use of thrombin, this suggests that the 13-TCP ・ PRP complex alone will promote osteogenesis when used in clinical treatments. In addition this method, which uses no animal-derived thrombin, could have additional benefits in a clinical setting, preventing infection by unknown viruses or the side effects of abnormal blood coagulation.
In 4 or 8 weeks, there was much increase of bone the PRP/β -TCP complex and CCEF stimulus modelthan PRP/β -TCP complex. Precise-ization of the bone and new bone formation promotion effect of the CCEF stimulating method was accepted.