2003 Fiscal Year Final Research Report Summary
Development of β-oxidation measurement for evaluating energy metabolism in the heart and its application to heart diseases.
| Project/Area Number |
14572153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
TAKAHASHI Toshihiro NIIGATA UNIVERSITY, Radioisotope Center, Associate Professor, アイソトープ総合センター, 助教授 (70143039)
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| Co-Investigator(Kenkyū-buntansha) |
IZUMIKAWA Takuji NIIGATA UNIVERSITY, Radioisotope Center, Assistant, アイソトープ総合センター, 助手 (60282985)
NAITOH Makoto NIIGATA UNIVERSITY, Graduate School of Medical anddental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (30045786)
WATANABE Ken-ichi NIIGATA UNIVERSITY, Department of clinical Pharmacology, Niigata College of Pharmacy, Professor, 臨床薬理学教室, 教授 (70175090)
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| Project Period (FY) |
2002 – 2003
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| Keywords | heart muscle / fatty acid / β-oxidation / two-dimensional TLC / storage type metabolite / β-oxidation type metabolite / chronic heart failure |
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| Research Abstract |
In this study, we propose the new analytical method of metabolites in the heart using labeled fatty acid derivatives and the myocardial function study by the quantitative assessment of the separated metabolic fractions. The used radiopharmaceutical is 9MPA(15-(p-[1-125]iodophenyl)-9-methylpenta decanoic acid), which is designed to inhibit the β-oxidative metabolic process with containing methyl-branch at the odd-numbered position. In result, we have demonstrated the separation system of myocardial metabolites by two-dimensional TLC method, which showed the well-separation of the main two type myocardial metabolites --storage type metabolites(triglycerides) and β-oxidation type metabolites --on the same TLC plates and then have shown the evaluating method of the myocardial function by the radioactivity measurement of each separated fraction. Further, we have applied the above method to the CHF(chronic heart failure) heart, which is a rat model of dilated cardiomyopathy induced by autoimmune myocarditis. It has been shown that in the CHF heart, the storage metabolic route was mainly damaged.
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Research Products
(10 results)
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[Publications] Takahashi T., Sega T., Watanabe K., Wahed M.I.I., Narasimman G., Saito Y., Abe Y., Hirabayashi K., Shirai K., Ma M., Juan W., Tachikawa H.: "Analysis of myocardial fatty acid metabolites"NIIGATA KAKUIGAKU KONWAKAI(Abstract). Vol.31. 27-31 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takahashi T., Watanabe K., Tachikawa H., Sega T., Wahed M.I.I., Narasimman G., Saito Y., Abe Y., Hirabayashi K., Shirai K., Ma M., Juan W., Tazawa S., Ashino H., Inoue M.: "Analysis of myocardial fatty acid metabolites in rats with heart failure"The Pharmaceutical Society of Japan, Annual Meeting(Abstract). Vol.3, No.123. 66 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takahashi T., Sega T., Watanabe K., Wahed M.I.I., Ihoue M., Saito Y., Tazawa S., Ashino H., Inoue M.: "Analysis of myocardial fatty acid metabolites by TLC method. -Evaluation of myocardial energy-metabolic function in rats with heart failure before/after medical treatment-"The Pharmaceutical Society of Japan, Annual Meeting(Abstract). Vol.3, No.122. (2002)
Description
「研究成果報告書概要(欧文)」より
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