2003 Fiscal Year Final Research Report Summary
Expression proteomics of angiogenesis-modulated factors in human vitreous humors derived from diabetic retinopathy
| Project/Area Number |
14572190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Osaka Medical College |
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Principal Investigator |
NAKANISHI Toyofumi Osaka Medical College, Faculty of Medicine, Assistant Professor, 医学部, 講師 (10247843)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Tsunehiko Osaka Medical College, Dept.of Ophthalmology, Professor, 医学部, 教授 (70222891)
SHIMIZU Akira Osaka Medical College, Dept.of Clin.Patholog., Professor, 医学部, 教授 (00028581)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Expression Proteomics / Diabetic Retinopathy / Angiogenesis-modulated factor / Vitreous humor / Ion-trap MS / PEDF |
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| Research Abstract |
A catalogue of proteins in the human vitreous humor may contribute to elucidating the pathogenesis of various diseases in ophthalmology. To improve the recovery of proteins in vitreous, we applied one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-PAGE). Proteins were extracted from unstained gel strips and digested in gel with trypsin and the peptides were analyzed by capillary-column reversed-phase high-performance liquid chromatography coupled with electrospray ionization-ion trap-mass spectrometry. From a patient with diabetic retinopathy, 84 different proteins were identified. Most of the proteins which we identified in vitreous previously using 2D-PAGE were also identified in the present study. In total, we identified 121 different proteins including five proteins seen at the genomic level only. Four angiogenic factors, insulin-like growth factor, vascular endothelial growth factor, fibroblast growth factor, and placental endothelial cell growth factor, and three anti-angiogenic factors, pigment epithelium-derived factor, endostatin, and thrombospondin, were found, and this may contribute to elucidating the pathological changes in the concentration and the modified structures of these proteins, in diseases of the retina, especially, diabetic retinopathy.
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Research Products
(4 results)
