2004 Fiscal Year Final Research Report Summary
Biological and genetic analysis of Vibrio vulnificus in the slime of tidal flats, seawater, and fishes colleted along Ariake Sea, Japan
| Project/Area Number |
14572194
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kurume University |
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Principal Investigator |
SAGAWA Kimitaka Kurume University, School of Medicine, Department of Laboratory Medicine, Professor, 医学部, 教授 (20140650)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Vibrio vulnificus / Ariake Sea / hemolytic activity / hemolysis / Chikugo River / thiourea / pulsed-field gel electrophoresis / Colia nasus |
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| Research Abstract |
To investigate the seasonal change of Vibrio vulnificus in Ariake Sea, Japan, we attempted to isolate V.vulnificus from the slime of tidal flats, seawater, and fishes obtained from three harbors along Ariake Sea. The samples were collected twice a month from January to December,2001. Also, we determined the biological and genetic characteristics of the individual isolates.
V.vulnificus were isolated throughout the year, but the isolation ratios were higher during the warmer season from June to October. The isolates in the warmer season were able to grow in culture media containing 0.5% NaCl, whereas those isolated in the remaining seasons could not. Moreover, the isolates in the warmer season showed greater hemolytic activity than those isolated in the remaining seasons. Those results suggest that V.vulnificus isolated in the warmer seasons are more vigorous in nature than those isolated in the remaining seasons.
Genetic analysis by pulsed-field gel electrophoresis using 50microM thiourea was successfully achieved. However, no identical band pattern was detected among strains studied.
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