| Project/Area Number |
14580755
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Osaka Bioscience Institute |
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Principal Investigator |
HAYAISHI Osamu Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Research Associate, 分子行動生物学部門, 研究員 (40025507)
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| Co-Investigator(Kenkyū-buntansha) |
SAKATA Mie Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Postdoctoral Associate, 分子行動生物学部門, 研究員 (10353525)
URADA Yoshihiro Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Head, 分子行動生物学部門, 研究部長 (10201360)
EGUCHI Naomi Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Vice Head, 分子行動生物学部門, 研究副部長 (10250086)
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| Project Period (FY) |
2002 – 2003
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| Keywords | inflammation / sytokine / IL-1β / TNF-α / Sleep / Prostaglandin / knockout mouse |
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| Research Abstract |
Infectious illness is associated with an increase in the amount of sleep and causes the production of proinflammatory cytokines and induction of cyclooxygenase-2 (COX-2), which is the key enzyme of the biosynthesis of prostaglandins (PGs). Both IL-1β and PGD_2 promote sleep in rats and mice. Pretreatment of COX-2 inhibitor reportedly blocked the IL-1β-promoted sleep in rats. Therefore, PGs are considered to mediate the somnogenic effect of IL-1β in rats. However, little is known about the molecular interaction between PGs and cytokines in the sleep-wake regulation in mice. Here, we employed the PGD_2 receptor-knockout mice and highly selective COX-2 inhibitor NS-398 to examine the involvement of PGD2 in the somnogenic effect of IL-1β in mice. IL-1β increased total amount of non-rapid eye movement (NREM) sleep in both wild-type (WT) and PGD_2 receptor-knockout mice and concomitantly reduced body temperature and locomotor activity for 6h after the intraperitoneal injection at 8 p.m. Pretreatment ofNS-398 inhibited neither spontaneous nor IL-1β-induced sleep in WT mice. These results indicate that PGD_2 is not involved in the somnogenic effect of IL-1β in mice.
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