2002 Fiscal Year Annual Research Report

Preconditioningによる肝阻血再灌流障害抑制の検討

Research Project

Project/Area Number	14770614
Research Institution	The University of Tokyo
Principal Investigator	大久保貴生東京大学, 医学部附属病院, 助手 (10334393)
Keywords	阻血再潅流障害 / 肝移植 / Preconditioning / NGFI-B / RT-PCR / 肝障害抑制
Research Abstract	ヒトおよびラットでNGFI-Bサブファミリーが肝阻血再潅流障害において発現し、かつ障害の程度を反映する遺伝子である事を発見した(Ohkubo T, et al. Early induction of nerve groth factor-induced genes after liver resection-reperfusion injury. Journal of Hepatology 2002)。これらNGFI-Bサブファミリーに属する遺伝子は転写調節因子として働き、アポトーシスに重要な関連を持つとされている。近年肝阻血再潅流障害の抑制法の一つとしてpreconditioning(短時間肝阻血再潅流)が注目されている。しかし、その基礎的背景は今だ明らかでない。preconditioning下における肝阻血再潅流障害抑制効果をNGFI-Bサブファミリー遺伝子をとおして明らかにするのが本研究の目的である。ラット肝阻血再潅流モデルを用いて、その詳細な肝障害機序の解析を行っている。オリゴヌクレオチドプライマーを使いたreverse transcription-polymerase chain reaction(RT-PCR)法にてpreconditioningの有無によるNGFI-Bサブファミリー遺伝子の発現を検討し、internal standard RNAを用いた定量RT-PCR法にて定量している。今後、臨床例でもpreconditioningによる肝阻血再潅流障害抑制効果を検証する予定である。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Ohkubo T, et al.: "Successful pancreatic duct-to jejunum anastomosis in a patient with a double pancreatic duct"Hepatogastroenterology. 49(48). 1724-1725 (2002)
[Publications] Ohkubo T, et al.: "Early induction of nervegrowth factor-induced gene after liver resection-reperfusion injury"J Hepatol. 36・2. 210-217 (2002)
[Publications] Kobayashi T, Ohkubo T, et al.: "Effect of amrinone on hepatic ischemia-reperfusion injury in rats"J Hepatol. 37・1. 31-38 (2002)
[Publications] Sugawara Y, Ohkubo T, et al.: "Vein reconstruction in modified right liver graft for living doner liver transplantation"Ann Surg. 237・2. 180-185 (2003)
[Publications] Imamura H, Ohkubo T, et al.: "Risk factors contributing to early and late phase intrahepatic recurrence of hepatocellular carcinoma after hepatecty"J Hepatol. 38・2. 200-207 (2003)
[Publications] Sugawara Y, Ohkubo T, et al.: "Living-donor liver transplantation in an HIV-positol patient with hemaphilia"Transplantation. 15:74-11. 1655-1656 (2002)
[Publications] Cescon M, Ohkubo T, et al.: "Thrombectomy of portal vein thonbosis in living doner liver transplantation"Abdom Imaging. 28・1. 60-61 (2003)
[Publications] Kaneko J, Ohkubo T, et al.: "Successful conservative therpy for portal vein thonbosis in living doner liver transplantation"Abdom Imaging. 28・1. 58-59 (2003)
[Publications] Kaneko J, Ohkubo T, et al.: "Normal splenic volume in adults by conputed tomopraphy"Hepatogastroenterology. 49・48. 1726-1727 (2002)
[Publications] Sugawara Y, Ohkubo T, et al.: "Living donor liver transplantation inadults"Surgery. 133・2. 348-352 (2002)
[Publications] Leelaudomlipi S, Ohkubo T, et al.: "Volumetric analysis of liver segments in 155 living donors"Liver Tramspl. 8・7. 612-614 (2002)
[Publications] Sugawara Y, Ohkubo T, et al.: "Successful treatment of hepatic C virus after liver transplantation from an identeal twin"Transplantation. 15:73-11. 1850-1851 (2002)
[Publications] Sugawara Y, Ohkubo T, et al.: "Duct to Duct biliary recenstruction in living-related liver transplantation"Transplantation. 27:73-8. 1348-1350 (2002)
[Publications] Cescon M, Ohkubo T, et al.: "Right liver graft without middle hepatic vein reconstruction from a living donor"Transplantation. 15:73-7. 102-106 (2002)
[Publications] 大久保貴生: "肝移植の現状"成人病と生活習慣病. 33・1. 47-57 (2003)
[Publications] 大久保貴生: "移植後感染症"Annual Review 消化器2003. 99 (2003)

2002 Fiscal Year Annual Research Report

Preconditioningによる肝阻血再灌流障害抑制の検討

Principal Investigator

大久保 貴生 東京大学, 医学部附属病院, 助手 (10334393)

Research Products

[Publications] Ohkubo T, et al.: "Successful pancreatic duct-to jejunum anastomosis in a patient with a double pancreatic duct"Hepatogastroenterology. 49(48). 1724-1725 (2002)

[Publications] Ohkubo T, et al.: "Early induction of nervegrowth factor-induced gene after liver resection-reperfusion injury"J Hepatol. 36・2. 210-217 (2002)

[Publications] Kobayashi T, Ohkubo T, et al.: "Effect of amrinone on hepatic ischemia-reperfusion injury in rats"J Hepatol. 37・1. 31-38 (2002)

[Publications] Sugawara Y, Ohkubo T, et al.: "Vein reconstruction in modified right liver graft for living doner liver transplantation"Ann Surg. 237・2. 180-185 (2003)

[Publications] Imamura H, Ohkubo T, et al.: "Risk factors contributing to early and late phase intrahepatic recurrence of hepatocellular carcinoma after hepatecty"J Hepatol. 38・2. 200-207 (2003)

[Publications] Sugawara Y, Ohkubo T, et al.: "Living-donor liver transplantation in an HIV-positol patient with hemaphilia"Transplantation. 15:74-11. 1655-1656 (2002)

[Publications] Cescon M, Ohkubo T, et al.: "Thrombectomy of portal vein thonbosis in living doner liver transplantation"Abdom Imaging. 28・1. 60-61 (2003)

[Publications] Kaneko J, Ohkubo T, et al.: "Successful conservative therpy for portal vein thonbosis in living doner liver transplantation"Abdom Imaging. 28・1. 58-59 (2003)

[Publications] Kaneko J, Ohkubo T, et al.: "Normal splenic volume in adults by conputed tomopraphy"Hepatogastroenterology. 49・48. 1726-1727 (2002)

[Publications] Sugawara Y, Ohkubo T, et al.: "Living donor liver transplantation inadults"Surgery. 133・2. 348-352 (2002)

[Publications] Leelaudomlipi S, Ohkubo T, et al.: "Volumetric analysis of liver segments in 155 living donors"Liver Tramspl. 8・7. 612-614 (2002)

[Publications] Sugawara Y, Ohkubo T, et al.: "Successful treatment of hepatic C virus after liver transplantation from an identeal twin"Transplantation. 15:73-11. 1850-1851 (2002)

[Publications] Sugawara Y, Ohkubo T, et al.: "Duct to Duct biliary recenstruction in living-related liver transplantation"Transplantation. 27:73-8. 1348-1350 (2002)

[Publications] Cescon M, Ohkubo T, et al.: "Right liver graft without middle hepatic vein reconstruction from a living donor"Transplantation. 15:73-7. 102-106 (2002)

[Publications] 大久保貴生: "肝移植の現状"成人病と生活習慣病. 33・1. 47-57 (2003)

[Publications] 大久保貴生: "移植後感染症"Annual Review 消化器2003. 99 (2003)

大久保貴生東京大学, 医学部附属病院, 助手 (10334393)