2003 Fiscal Year Annual Research Report

脂肪細胞の肥大化をモニターする分子機構の解析

Research Project

Project/Area Number	15081205
Research Institution	Kyoto University
Principal Investigator	河田照雄京都大学, 農学研究科, 助教授 (10177701)
Co-Investigator(Kenkyū-buntansha)	都築巧京都大学, 助手 (50283651) 井上和生京都大学, 助手 (80213148)
Keywords	肥満 / 脂肪細胞 / 生活習慣病 / イオンチャネル / センサー / アディポサイトカイン
Research Abstract	脂肪細胞は、第一義的な機能である「脂肪を貯める」ために、その直径が数ミクロンから200ミクロン位まで変化し他に類をみないほどの容積変化に富む細胞である。このことが生体のエネルギー供給の保証機構ともなっている。しかしながら、近年脂肪細胞の過度の発達、即ち肥大化がアディポサイトカインと呼ばれる各種の生活習慣病の直接的な誘発因子を脂肪細胞自身から分泌させることが判明してきた。従って、脂肪細胞の発達(肥大化)とその制御機構の解明の重要性が指摘される。本研究においては、既に担当者らが予備的に見いだした脂肪細胞でのCa2+による応答性ならびに容積感受性イオンチャネル遺伝子情報を指標として、脂肪細胞の発達、肥大化を制御する膜内在性の機能分子(モニター分子)の検索を行った。その結果、脂肪細胞にある種の容積感受性イオンチャネルが発現していることを見いだした。現在、分子生物学的およびタンパク化学的手法を用いて、その分子の詳細な構造や性状の解析を行っている。また、容積増大と関連する脂肪酸による細胞レベルでのカルシウムイオンの応答性を共焦点レーザ顕微鏡などを用いて測定し、容積感受性イオンチャネルとの関連を検討した。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Kamei Y, et al.: "Identification of PPARγ coactivator 1β/ERR ligand 1 as an estrogen-related receptor protein ligand, whose expression induces a high-energy expenditure and antagonizes obesity in mice."Proc.Natl.Acad.Sci.U.S.A.. 100. 12378-12383 (2003)
[Publications] Takahashi N, et al.: "Abietic acid activates peroxisome proliferator-activated receptor-γ(PPARγ) in RAW264.7 macrophages and 3T3-L1 adipocytes to regulate gene expression involved in inflammation and lipid metabolism."FEBS Lett.. 550. 190-194 (2003)
[Publications] Kim C.-S, et al.: "Macrophage inflammatory protein-related protein-2, a novel CC chemokine, can regulate preadipocyte migration and adipocyte differentiation."FEBS Lett.. 548. 125-130 (2003)
[Publications] Kamei Y, et al.: "A forkhead transcription factor FKHR up-regulates lipoprotein lipase expression in skeletal muscle"FEBS Lett.. 536. 232-236 (2003)
[Publications] Kim C-S, et al.: "Capsaicin exhibits anti-inflammatory property by inhibiting IkB-α degradation in LPS-stimulated peritoneal macrophages."Cellular Signalling. 15. 299-306 (2003)
[Publications] Kageyama H, et al.: "Fasting increases gene expressions of uncoupling proteins and peroxisome proliferator-activated receptor-γ in brown adipose tissue of ventromedial hypothalamus-lesioned rats."Life Sciences. 72. 3035-3046 (2003)
[Publications] Mae T, et al.: "A licorice ethanolic extract with PPAR-γ ligand-binding activity affects diabetes in KK-Ay mice, abdominal obesity in diet-Induced obese C57BL mice and Hypertension in spontaneously hypertensive rats."J.Nutrition. 133. 3369-3377 (2003)
[Publications] Masuda Y, et al.: "Up-regulation of uncoupling proteins (UCPs) by oral administration of capsiate, a non-pungent capsaicin analog."J.Appl.Physiol.. 95. 2408-2415 (2003)
[Publications] Kuroda M, et al.: "Phenolics with PPAR-γ, ligand-binding activity obtained, from Licorice (Glycyrrhiza uralensis roots) and ameliorative effects of glycilin on genetically diabetic KK-A^y mice."Bioorg.Med.Chem.Lett.. 13. 4267-4272 (2003)
[Publications] Yu R, et al.: "Involvement of leukotactin-1, a novel CC chemokine, in human atherosclerosis."Atherosclerosi. (In press). (2004)
[Publications] Tsuda T, et al.: "Anthocyanin enhances adipocytokine secretion and adipocyte specific gene expression in Isolated Rat adipocytes."Biochem.Biophys.Res.Commun. 316. 149-157 (2004)

2003 Fiscal Year Annual Research Report

脂肪細胞の肥大化をモニターする分子機構の解析

Principal Investigator

河田 照雄 京都大学, 農学研究科, 助教授 (10177701)

Research Products

[Publications] Kamei Y, et al.: "Identification of PPARγ coactivator 1β/ERR ligand 1 as an estrogen-related receptor protein ligand, whose expression induces a high-energy expenditure and antagonizes obesity in mice."Proc.Natl.Acad.Sci.U.S.A.. 100. 12378-12383 (2003)

[Publications] Takahashi N, et al.: "Abietic acid activates peroxisome proliferator-activated receptor-γ(PPARγ) in RAW264.7 macrophages and 3T3-L1 adipocytes to regulate gene expression involved in inflammation and lipid metabolism."FEBS Lett.. 550. 190-194 (2003)

[Publications] Kim C.-S, et al.: "Macrophage inflammatory protein-related protein-2, a novel CC chemokine, can regulate preadipocyte migration and adipocyte differentiation."FEBS Lett.. 548. 125-130 (2003)

[Publications] Kamei Y, et al.: "A forkhead transcription factor FKHR up-regulates lipoprotein lipase expression in skeletal muscle"FEBS Lett.. 536. 232-236 (2003)

[Publications] Kim C-S, et al.: "Capsaicin exhibits anti-inflammatory property by inhibiting IkB-α degradation in LPS-stimulated peritoneal macrophages."Cellular Signalling. 15. 299-306 (2003)

[Publications] Kageyama H, et al.: "Fasting increases gene expressions of uncoupling proteins and peroxisome proliferator-activated receptor-γ in brown adipose tissue of ventromedial hypothalamus-lesioned rats."Life Sciences. 72. 3035-3046 (2003)

[Publications] Mae T, et al.: "A licorice ethanolic extract with PPAR-γ ligand-binding activity affects diabetes in KK-Ay mice, abdominal obesity in diet-Induced obese C57BL mice and Hypertension in spontaneously hypertensive rats."J.Nutrition. 133. 3369-3377 (2003)

[Publications] Masuda Y, et al.: "Up-regulation of uncoupling proteins (UCPs) by oral administration of capsiate, a non-pungent capsaicin analog."J.Appl.Physiol.. 95. 2408-2415 (2003)

[Publications] Kuroda M, et al.: "Phenolics with PPAR-γ, ligand-binding activity obtained, from Licorice (Glycyrrhiza uralensis roots) and ameliorative effects of glycilin on genetically diabetic KK-A^y mice."Bioorg.Med.Chem.Lett.. 13. 4267-4272 (2003)

[Publications] Yu R, et al.: "Involvement of leukotactin-1, a novel CC chemokine, in human atherosclerosis."Atherosclerosi. (In press). (2004)

[Publications] Tsuda T, et al.: "Anthocyanin enhances adipocytokine secretion and adipocyte specific gene expression in Isolated Rat adipocytes."Biochem.Biophys.Res.Commun. 316. 149-157 (2004)

河田照雄京都大学, 農学研究科, 助教授 (10177701)