2007 Fiscal Year Final Research Report Summary
Functional Genomics Approach to Exploiting Molecular Diversity
| Project/Area Number |
15101007
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
EBIZUKA Yutaka The University of Tokyo, Graduate School of Pharmaceutical Sciences, Professor (90107384)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBUYA Masaaki The University of Tokyo, Graduate School of Pharmaceutical Sciences, Associate Professor (50170923)
KUSHIRO Tetsuo The University of Tokyo, Graduate School of Pharmaceutical Sciences, Assistant Professor (80373299)
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|---|
| Project Period (FY) |
2003 – 2007
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| Keywords | polyketides / triperpenes / triterpene synthases / polyketide synthases / molecular diversity / biosynthesis / functional genomics |
|---|
| Research Abstract |
(1) By utilizing genomic information, twenty four new triterpene synthase genes were cloned from higher plants, ferns and filamentous fungi. Their enzyme functions were identified by heterologous expression in yeast.
(2) As the products of these triterpene synthases, three new triterpenes with novel skeletons were identified.
(3) Lanosterol synthase genes were discovered for the first time from the plant kingdom and the presence of biosynthetic pathway of phytosterols via lanosterol was demonstrated.
(4) By reacting triterpene synthases with non-physiological substrates and synthetic substrate analogues, five new polyprenoids were obtained
(5) Each three novel oxidases and sugartransferases were cloned and their functions identified as the triterpene modifying enzymes.
(6) Eleven new iterative polyketide synthase (i-PKS) genes were cloned from filamentous fungi. Their enzyme functions were identified by expression in a heterologous fungal strain.
(7) Six new polyketide compounds with unique structures were identified as the products of these i-PKSs.
(8) Four Type-III i-PKS genes were identified in the genome of Asp. oryzae and one of them was demonstrated to encode an orsellinic acid synthase.
(9) As the higher structure for Type-I i-PKSs, a novel homo-tetramer model was proposed.
(10) As the novel polyketide modifying enzymes, a chain-shortening enzyme, a dehydratase, an oxidase with cycloaddition activity and two prenyltrasferase were cloned and their enzyme functions identified.
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Research Products
(291 results)
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[Journal Article] Remarkably Different Structures and Reactions of Ketoreductases for the Opposite Stereochemical Control in the Biosynthesis of BIQ Antibiotics2004
Author(s)
Takaaki Taguchi, Kanako Kunieda, Mayuko Takeda-Shitaka, Daisuke Takaya, Noriaki Kawano, Meriel R. Kimberley, Kevin I. Booker-Milburn, G. Richard Stephenson, Hideaki Umeyama, Yutaka Ebizuka, Koji Ichinose
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Journal Title
Bioorg. Med. Chem 12
Pages: 5917-5927
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Triterpene Biosynthetic Gene Cluster from Fungi2007
Author(s)
Tetsuo Kushiro, Hisashi Mitsuguchi, Yasuyo Seshime, Isao Fujii, Masaaki Shibuya, Yutaka Ebizuka
Organizer
The 4th Takeda Science Foundation Symposium on PharmaSciences "On the Frontiers of Chemical biology
Place of Presentation
Tokyo, Japan
Year and Date
2007-12-03
Description
「研究成果報告書概要(欧文)」より
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[Presentation] New Biosynthetic Pathway of Phytosterol via Lanosterol2007
Author(s)
Kiyoshi Ohyama, Masashi Suzuki, Xiang Ting, Hikaru Seki, Jun Kikuchi, Tetsuo Kushiro, Masaaki Shibuya, Yutaka Ebizuka, Kazuki Saito, Toshiya Muranaka
Organizer
49th Sympsium on the Chemistry of Natural Products
Place of Presentation
Sapporo, Japan
Year and Date
2007-09-19
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Biosynthetic Pathway of Phytosterols via Lanosterol2006
Author(s)
Kiyoshi Ohyama, Masashi Suzuki, Ting Xiang, Hikaru Seki, Jun Kikuchi, Kazuki Saito, Toshiya Muranaka, Yuji Katsube, Tetsuo Kushiro, Masaaki Shibuya, Yutaka Ebizuka
Organizer
ICOB-5 & ISCNP-25 IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto, Japan
Year and Date
2006-07-25
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Lanosterol Synthase from Arabidopsis thaliana2005
Author(s)
Ting Xiang, Masaaki Shibuya, Yutaka Ebizuka, Masashi Suzuki, Toshiya Muranaka
Organizer
2005International Chemical Congress of Pacific Basin Society, Biosynthesis of Natural Products Symposium
Place of Presentation
Honolulu, USA
Year and Date
2005-12-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Functional Analysis of Fungal Polyketide Synthases2004
Author(s)
Isao Fujii
Organizer
The Fall Conference of the Korean Society of Applied Biological Chemistry on the New Waves in Microbiology, -Biodegradation, Biotransformation, Biocatalysis
Place of Presentation
Daecheon, Korea
Year and Date
2004-10-22
Description
「研究成果報告書概要(欧文)」より
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