2007 Fiscal Year Final Research Report Summary
An exhaustive exploration of the genes involved in molluscan shell formation : a primer for a genome projec
| Project/Area Number |
15104009
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Stratigraphy/Paleontology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ENDO Kazuyoshi University of Tsukuba, Graduate School of Life and Environmental Sciences, Associate Professor (80251411)
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| Co-Investigator(Kenkyū-buntansha) |
KURATANI Shigeru Center for Developmental Biology, Evolationary Morphology, Research leader (00178089)
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| Project Period (FY) |
2003 – 2007
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| Keywords | evolution / development / animal / genome / gene / biomineralization / morohoeenesis / molluscs |
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| Research Abstract |
In this study, we aimed at elucidating the evolutionary processes and mechanisms of the shell by characterizing the (1) master genes shell formation, (2) proteins controlling shell (biomineralization), and (3) control precipitation genes for the shell left-right asymmetry, and obtained the following results.
(1) master genes for shell formation Gene expression patterns of the transcription factor engrailed and the growth factor dpp in Lymnaea stagnalis indicated that engrailed is expressed around the shell gland just like in other molluscs, and dpp is expressed only in the right-hand side of the invaginated shell gland, suggesting that both genes are involved in shell formation and dpp could be further responsible for the shell left-right asymmetry.
(2) biomineralization genes Phylogenetic analysis of the shell matrix protein Dermatopontin among eight species of pulmonate snails showed that the gene duplications that lead to the evolution of the shell matrix proteins occurred at least twice independently in pulmonates. Comparisons with the fossil record indicated that these duplications should have occurred after Mesozoic Era. In the pearl oyster Pinctada fucata, the primary structure of the unusually acidic shell matrix protein Aspein has been deduced. Analyses of expression patterns and in vitro functions demonstrated that Aspein is highly likely the factor controlling the selective precipitation of calcite shells in P. fucata.
(3) control genes for the shell left right asymmetry We performed an exhaustive analysis using the HiCEP method for the mRNAs that show asymmetric expression patterns between the right-hand side and the left-hand side of the mantle epithelium in Lymnaea stagnalis, and found that a total of 11588 genes are expressed in the mantle and that dozens of genes show a left-right asymmetric gene expression pattern. Analysis is still on progress as to the structure and function for the 20 genes that indicated the highest extent of asymmetry.
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Research Products
(99 results)
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[Journal Article] Evidence for novel iron sulfide mineralization mediated by a hot-vent gastropod in protective iron armor2006
Author(s)
Suzuki, S., Kopp, R. E., Kogure, T., Suga, A., Takai, K., Tsuchida, S., Ozaki, N., Endo, K, Hashimoto, J., Kato, Y, Mizota, C., Hirata, T., Chiba, H., Nealson, K. H., Horikoshi K. and Kirschvink, J. L.
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Journal Title
Earth & Planetary Science Letter 242
Pages: 39-50
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular Evolution and Functionally Important Structures of Molluscan Dermatopontin : Implications for the Origins of Molluscan Shell Matrix Proteins2006
Author(s)
Sarashina, I., Yamaguchi, H., Haga, T., Iijima, M., Chiba, S. and Endo, K
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Journal Title
Journal of Molecular Evolution 62
Pages: 307-318
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Lymnaea stagnalis as a Model Organism for Studies of Calcium Carbonate Biomineralization, In Kobayashi, I.(ed)2004
Author(s)
Endo, K., Sarashina, I., and Asami,T.
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Journal Title
Biomineralization : formation, diversity, evolution and application, Tokai Univ (press)
Pages: 172-175
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Molecular phylogeny of acantharian and polycystine Radiolaria. INTERRAD XI2006
Author(s)
Kunitomo, Y., Sarashina, I., Iijima, M., Endo, K. and Sashida, K.
Organizer
RADIOLARIANS IN STRATIGRAPHY & PALEOCEANOGRAPHY 11th Meeting of the International Association of Radiolarian Paleontologists & CIRCU M-PACIFIC TRIASSIC STRATIGRAPHY & CORRELATION A symposium hosted by IGCP 467 and STS Wellington
Place of Presentation
New Zealand
Year and Date
20060319-24
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Yamamoto, A., Kato, Y. and Endo, K., Microbial structures from Pliocene sedimentary iiron deposits in Kawatana and Sonogi Mines, Nagasaki, southern Japan2003
Author(s)
Iijima, M., Akiba, N., Sarashina, I., Kuratani, S. and Endo, K.
Organizer
16th International Symposium on Environmental Biogeochemistry
Place of Presentation
Oirase(Aomori), Japan
Year and Date
20030901-06
Description
「研究成果報告書概要(欧文)」より
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