2006 Fiscal Year Final Research Report Summary
Development of Highly Sensitive, Accurate, and Quantitative Analytical System for Proteome
Project/Area Number |
15201043
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied genomics
|
Research Institution | Osaka University |
Principal Investigator |
OKAMURA Taka-aki Osaka University, Graduate School of Science, Associate Professor, 大学院理学研究科, 講師 (90252569)
|
Co-Investigator(Kenkyū-buntansha) |
UEYAMA Norikazu Osaka University, Graduate School of Science, Researcher, 大学院理学研究科, 特任研究員 (80093376)
NAKAZAWA Takashi Nara Women's University, Department of Chemistry, Associate Professor, 理学部, 助教授 (30175492)
TSUNASAWA Susumu Shimadzu Corporation, Life Science Laboratory, Senior R & D Manager, 分析計測事業部ライフサイエンス研究所, 部長(研究職) (30029962)
KUYAMA Hiroki Osaka University, Institure for Protein Research, Associate Professor, 蛋白質研究所, 寄附研究部門助教授 (60437332)
|
Project Period (FY) |
2003 – 2006
|
Keywords | Proteome / Metal Complex / Protein / Mass Analysis / Amino Acid Sequencing / N-terminal Peptide / C-terminal Peptide / Labeling Reagent |
Research Abstract |
As a post-genome project, proteome analysis is one of the most important problems. Generally, proteome analyses identify precursor proteins on genes by determining internal sequences of the target protein referred to genome data base. However, these methods can not determine accurately mature proteins working in important biological systems. In this project, the following results were obtained. 1. Establishment of a new method for isolation and characterization of N-terminal peptide 2. Development of N-terminal labeling reagent having ruthenium compound and analytical method of protein complexes using this reagent 3. Selective C-terminal modification of proteins and peptides and new analytical methods for proteome 4. Rapid and efficient MALDI-TOF MS peak detection of 2-nitrobenzenesulfenyl-labeled peptides using the combination of HPLC and an automatic spotting apparatus The first is selective N-terminal labeling by a reagent having biotin and disulfide moieties following isolation using avidin-biotin technique. The disulfide bond was oxidatively cleaved to release N-terminal peptide, which was analyzed by mass spectroscopy to give amino acid sequences. The second is selective labeling of proteins by ruthenium complex. After enzymatic digestion, the labeled N-terminal fragment was exclusively detected without requiring any separation. Using this reagent to a mixture of proteins afforded simultaneous detection of all the N-termini of constituent proteins. The next one is selective C-terminal labeling via oxazolone. intermediate, which enabled successful determination of C-terminal amino acid sequences. These original works in this project resulted in many reports, domestic and overseas patents, and commercialized products.
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Research Products
(254 results)
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[Patent(Industrial Property Rights)] タンパク質又はペプチドのC末端を修飾法、C末端固定化法、及び解析法2006
Inventor(s)
中澤 隆, 岡むつみ, 西田希実子, 山口実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 綱澤進
Industrial Property Rights Holder
中澤 隆, 岡むつみ, 西田希実子, 山口実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 綱澤進
Industrial Property Number
PCT/JP2006/321583
Filing Date
2006-10-23
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] タンパク質又はペプチドのC末端を修飾法、C末端固定化法、及び解析法2005
Inventor(s)
中澤 隆, 岡むつみ, 西田希実子, 山口実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 綱澤進
Industrial Property Rights Holder
中澤 隆, 岡むつみ, 西田希実子, 山口実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 綱澤進
Industrial Property Number
特願2005-307831
Filing Date
2005-10-21
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] Method for mass spectrometric determining amino acid sequence of a peptide based on coupling N-terminus of the peptide with a protective amino acid derivativ2004
Inventor(s)
山口 実, 安藤英治, 廉岡茂巳, 上山憲一, 岡村高明, 中澤 隆
Industrial Property Rights Holder
山口 実, 安藤英治, 廉岡茂巳, 上山憲一, 岡村高明, 中澤 隆
Industrial Property Number
US Pat. Appl. Publ. No. 2004180381
Filing Date
2004-03-10
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] Method for derivatizing protein or peptide to sulfonic acid derivative2004
Inventor(s)
乗岡茂巳, 山口 実, 九山浩樹, 小浜孝士, 中澤 隆, 安藤英治, 上山憲一, 岡村高明
Industrial Property Rights Holder
乗岡茂巳, 山口 実, 九山浩樹, 小浜孝士, 中澤 隆, 安藤英治, 上山憲一, 岡村高明
Industrial Property Number
US Pat. Appl. Publ. No. 2005084927
Filing Date
2004-10-13
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] Method for derivatizing protein or peptide to sulfonic acid derivative2004
Inventor(s)
乗岡茂巳, 山口 実, 九山浩樹, 小浜孝士, 中澤 隆, 安藤英治, 上山憲一, 岡村高明
Industrial Property Rights Holder
乗岡茂巳, 山口 実, 九山浩樹, 小浜孝士, 中澤 隆, 安藤英治, 上山憲一, 岡村高明
Industrial Property Number
EP Pat. Appl. Publ. No. 1561755
Filing Date
2004-10-13
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] Novel metal complex, and its use in protein amino acid sequence determination method2003
Inventor(s)
上山憲一, 岡村高明, 乗岡茂巳, 中澤隆, 九山浩樹, 安藤英治
Industrial Property Rights Holder
上山憲一, 岡村高明, 乗岡茂巳, 中澤隆, 九山浩樹, 安藤英治
Industrial Property Number
US Pat. Appl. Publ. No. 20040029181
Filing Date
2003-07-08
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] Method for selectively collecting N-terminal peptide fragment of protein2003
Inventor(s)
乗岡茂巳, 上山憲一, 岡村高明, 中澤 隆, 山口 実, 安藤英治
Industrial Property Rights Holder
乗岡茂巳, 上山憲一, 岡村高明, 中澤 隆, 山口 実, 安藤英治
Industrial Property Number
US 7, 041, 472 B2
Filing Date
2003-12-19
Acquisition Date
2006-05-09
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] タンパク質又はペプチドをスルホン酸誘導体化する方法2003
Inventor(s)
乗岡茂巳, 山口 実, 九山浩樹, 小浜孝士, 中澤 隆, 安藤英治, 上山憲一, 岡村高明
Industrial Property Rights Holder
乗岡茂巳, 山口 実, 九山浩樹, 小浜孝士, 中澤 隆, 安藤英治, 上山憲一, 岡村高明
Industrial Property Number
特許公開2005-139174
Filing Date
2003-10-16
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] タンパク質又はペプチドのC末端を修飾する方法2003
Inventor(s)
中澤 隆, 山口 実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 乗岡茂巳
Industrial Property Rights Holder
中澤 隆, 山口 実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 乗岡茂巳
Industrial Property Number
特許公開2005-139163
Filing Date
2003-10-17
Description
「研究成果報告書概要(和文)」より
-
[Patent(Industrial Property Rights)] Method for modifying protein or peptide C terminal2003
Inventor(s)
中澤 隆, 山口 実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 乗岡茂巳
Industrial Property Rights Holder
中澤 隆, 山口 実, 九山浩樹, 安藤英治, 上山憲一, 岡村高明, 乗岡茂巳
Industrial Property Number
US Pat. Appl. Publ. No. 2005085622
Filing Date
2003-12-19
Description
「研究成果報告書概要(和文)」より