2005 Fiscal Year Final Research Report Summary
The molecular mechanism of mitochondrial fission and fusion in higher plants.
| Project/Area Number |
15208001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TSUTSUMI Nobuhiro The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor (00202185)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZONO Mikio The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor (70282697)
KADOWAKI Koichi The University of Tokyo, Team Leader (60355724)
SAKAMOTO Wataru Okayama University, Institute of Plant Science and Resources, Professor (20222002)
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| Project Period (FY) |
2003 – 2005
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| Keywords | DRP3 / mitochondrial fusion / mitochondrial fission / Kaede |
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| Research Abstract |
The Arabidopsis genome has two similar dynamin-like proteins, DRP3a and DRP3b (76.7% identity). DRP3a is reported to be localized in chloroplasts [Kang et al. (1998) Plant Mol. Biol. 38 : 437], while DRP3b functions in mitochondrial division [Arimura and Tsutsumi (2002) Proc. Natl. Acad. Sci. USA 99 : 5727]. Using GFP fusion proteins, we observed both DRP3a and DRP3b in portions of mitochondria but not in chloroplasts. Furthermore, cells transformed with DRP3a and DRP3b with a defective GTPase domain had normal chloroplasts but elongated mitochondria. These results imply that both DRP3b and DRP3a are involved in the division of plant mitochondria.
The balance between mitochondrial fusion and fission influences the reticular shape of mitochondria in yeasts. Little is known about whether mitochondria fusion occurs in plants. Plant mitochondria are usually more numerous and more grain-shaped than animal mitochondria. blast searches of the nuclear and mitochondrial genome sequences of Arabidopsis thaliana did not find any obvious homologue of mitochondrial fusion genes found in animals and yeasts. To determine whether mitochondrial fusion actually occurs in plants, we labeled mitochondria in onion epidermal cells with a mitochondria-targeted, photoconvertible fluorescent protein Kaede and then altered the fluorescence of some of the mitochondria within a cell from green to red. Frequent and transient fusion of red and green mitochondria was demonstrated by the appearance of yellow mitochondria that subsequently redivided. We also show that mitochondrial fission occasionally occurs without an equal distribution of the nucleoid (DNA-protein complex in mitochondria), resulting in the coexistence of mitochondria containing various amounts of DNA within a single cell. The heterogeneity of DNA contents in mitochondria may be overcome by the frequent and transient fusion of mitochondria.
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Research Products
(46 results)
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[Journal Article] OsNAC6, a member of the NAC gene family, is induced by various stresses in rice2008
Author(s)
Ohnishi, T., Sugahara, S., Yamada, T., Kikuchi, K., Yoshiba, Y., Hirano, HY., Tsutsumi, N.
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Journal Title
GENES & GENETIC SYSTEMS 80
Pages: 135-139
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following re-aeration2006
Author(s)
Meguro, N., Tsuji, H., Suzuki, Y., Tsutsumi, N., Hirai, A., Nakazono, M.
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Journal Title
BREEDING SCIENCE 56
Pages: 365-370
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Conservation and diversification of meristem maintenance mechanism in Oryza sativa : Function of the FLORAL ORGAN NUMBER2 gene2006
Author(s)
Suzaki, T., Toriba, T., Fujimoto, M., Tsutsumi, N., Kitano, H., Hirano, HY.
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Journal Title
PLANT AND CELL PHYSIOLOGY 47
Pages: 1591-1602
Description
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[Journal Article] The mitochondrial fission regulator DRP3B does not regulate cell death in plants2006
Author(s)
Yoshinaga, K., Fujimoto, M., Arimura, S., Tsutsumi, N., Uchimiya, H., Kawai-Yamada, M.
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Journal Title
ANNALS OF BOTANY 97
Pages: 1145-1149
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Promoter shuffling at a nuclear gene for mitochondrial RPL27.Involvement of interchromosome and subsequent intrachromosome recombinations2006
Author(s)
Ueda, M., Arimura, S., Yamamoto, MP., Takaiwa, F., Tsutsumi, N., Kadowaki, K.
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Journal Title
PLANT PHYSIOLOGY 141
Pages: 702-710
Description
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[Journal Article] Anaconda, a new class of transposon belonging to the Mu superfamily, has diversified by acquiring host genes during rice evolution2005
Author(s)
Ohtsu, K., Hirano, HY., Tsutsumi, N., Hirai, A., Nakazono, M.
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Journal Title
MOLECULAR GENETICS AND GENOMICS 274
Pages: 606-615
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mammalian Bax initiates plant cell death through organelle destruction2005
Author(s)
Yoshinaga, K., Arimura, S., Hirata, A., Niwa, Y., Yun, DJ., Tsutsumi, N., Uchimiya, H., Kawai-Yamada, M.
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Journal Title
PLANT CELL REPORTS 24
Pages: 408-417
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mitochondrial behaviour in the early stages of ROS stress leading to cell death in Arabidopsis thaliana2005
Author(s)
Yoshinaga, K., Arimura, SI., Niwa, Y., Tsutsumi, N., Uchimiya, H., Kawai-Yamada, M.
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Journal Title
ANNALS OF BOTANY 96
Pages: 337-342
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tillering behavior of the rice fine culm 1 mutant2005
Author(s)
Goto, Y., Tanabe, M., Ishibashi, T., Tsutsumi, N., Yoshimura, A., Nemoto, K.
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Journal Title
PLANT PRODUCTION SCIENCE 8
Pages: 68-70
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Arabidopsis dynamin-like protein 2a(ADL2a), like ADL2b, is involved in plant mitochondrial division2004
Author(s)
Arimura, S., Aida, GP., Fujimoto, M., Nakazono, M., Tsutsumi, N.
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Journal Title
PLANT AND CELL PHYSIOLOGY 45
Pages: 236-242
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The rice pyruvate decarboxylase 3 gene, which lacks introns, is transcribed in mature pollen2004
Author(s)
Li, YH., Ohtsu, K., Nemoto, K., Tsutsumi, N., Hirai, A., Nakazono, M.
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Journal Title
JOURNAL OF EXPERIMENTAL BOTANY 55
Pages: 145-146
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Verma, DPS., A unified nomenclature for Arabidopsis dynamin-related large GTPases based on homology and possible functions2003
Author(s)
Hong, Z., Bednarek, SY., Blumwald, E., Hwang, I., Jurgens, G., Menzel, D., Osteryoung, KW., Raikhel, NV., Shinozaki, K., Tsutsumi, N., Verma, DPS.
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Journal Title
PLANT MOLECULAR BIOLOGY 53
Pages: 261-265
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re-aeration in rice2003
Author(s)
Tsuji, H., Meguro, N., Suzuki, Y., Tsutsumi, N., Hirai, A., Nakazono, M.
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Journal Title
FEBS LETTERS 546
Pages: 369-373
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase2003
Author(s)
Lee, J., Das, A., Yamaguchi, M., Hashimoto, J., Tsutsumi, N., Uchimiya, H., Umeda, M.
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Journal Title
PLANT JOURNAL 34
Pages: 417-425
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Organ-specific expressions and chromosomal locations of two mitochondrial aldehyde dehydrogenase genes from rice(Oryza sativa L.), ALDH2a and ALDH2b2003
Author(s)
Tsuji, H., Tsutsumi, N., Sasaki, T., Hirai, A., Nakazono, M.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
