2005 Fiscal Year Final Research Report Summary
The role of neuron-glia interaction in neuropathic pain
| Project/Area Number |
15209051
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | KYUSHU UNIVERCITY (2004-2005)
National Institute of Health Sciences (2003) |
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Principal Investigator |
INOUE Kazuhide Kyushu University, Faculty of Pharmaceutical Sciences, Professor, 大学院薬学研究院, 教授 (80124379)
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| Co-Investigator(Kenkyū-buntansha) |
KOIZUMI Schuichi National Institute of Health Sciences, Division of Pharmacology, 薬理部, 室長 (10280752)
TSUDA Makoto Kyushu University, Faculty of Pharmaceutical Sciences, Associate Professor, 大学院薬学研究院, 助教授 (40373394)
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| Project Period (FY) |
2003 – 2005
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| Keywords | ATP / P2X4 / microglia / BDNF / anion gradient / GABA |
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| Research Abstract |
In neuropathic pain animal models, spinal microglia become hypertrophic in their short and thick processes within the first 24 hrs after peripheral nerve injury. The time-course of microglial activation in the dorsal horn correlated with that of the development of tactile allodynia. Also we found that the microglia over-expressed P2X4 receptors and that P2X_4 stimulation of microglia is not only necessary for tactile allodynia, but is also sufficient to cause the allodynia (Nature 424,778-783,2003). Next we found that P2X_4 receptor activation is necessary to sustain the depolarizing shift in E_<anion> in rats with nerve injury, and that P2X4 stimulation evoked the release of BDNF from microglia. BDNF itself caused the depolarizing shift in E_<anion> in rat spinal neurons. Blocking the action of P2X4,BDNF caused the reduction of allodynia. These findings show that both the decrease in paw withdrawal threshold and the shift in E_<anion> in LI neurons caused by ATP-stimulated microglia through P2X_4 require BDNF-TrkB signaling and that the source of BDNF is the microglia themselves (Nature,438,1017-1021).
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[Journal Article] HX531, a retinoid X receptor antagonist, inhibited the 9-cis retinoic acid-induced binding with steroid receptor coactivator-1 using surface plasmon resonance.2005
Author(s)
T.Kanayasu-Toyoda, T.Fujino, T.Oshizawa, T.Suzuki, T.Nishimaki-Mogami, Y.Sato, J.Sawada, K.Inoue, K.Shudo, T.Yamaguchi
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Journal Title
J Steroid Biochem.Mol.Biol. 94
Pages: 303-309
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Riccardin C : a natural product that functions as a liver X receptor (LXR) agonist and an LXR antagonist2005
Author(s)
N.Tamehiro, Y.Sato, T.Suzuki, T.Hashimoto, Y.Asakawa, S.Yokoyama, T.Kawanishi, Y.Ohno, K.Inoue, T.Nagao, T.Nishimaki-Mogami
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Journal Title
FEBS Letters 579
Pages: 5299-5304
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity.2003
Author(s)
T.Moriyama, T.Iida, K.Kobayashi, T.Higashi, T.Fukuoka, H.Tsumura, C.Leon, N.Suzuki, K.Inoue, C.Gachet, K.Noguchi, M.Tominaga
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Journal Title
J. Neurosci. 23
Pages: 6058-6062
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity.2003
Author(s)
T.Moriyama, T.Iida, K.Kobayashi, T.Higashi, T.Fukuoka, H.Tsumura, C.Leon, N.Suzuki, K.Inoue, C.Gachet, K.Noguchi, M.Tominaga
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Journal Title
J.Neurosci. 23
Pages: 6058-6062
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Test of significant differences with a priori probability in microarray experiments.2003
Author(s)
K.Toda, S.Ishida, K.Nakata, R.Matsuda, Y.Shigemoto-Mogami, K.Fujishita, S.Ozawa, J.Sawada, K.Inoue, K.Shudo, Y.Hayashi
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Journal Title
Analytical Sciences 19
Pages: 1529-1535
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Potentiation of NMDA receptor-mediated synaptic responses by microglia.2003
Author(s)
Moriguchi S, Mizoguchi Y, Tomimatsu Y, Hayashi Y, Kadowaki T, Kagamiishi Y, Katsube N, Yamamoto K, Inoue K, Watanabe S, Nabekura J, Nakanishi H.
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Journal Title
Brain Res Mol Brain Res. 119
Pages: 160-169
Description
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