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2006 Fiscal Year Final Research Report Summary

Study on attenuation of Escherichia coli 0157 with a combination of essential oil of spices.

Research Project

Project/Area Number 15300254
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionThe University of Tokushima (2004-2006)
Nara Women's University (2003)

Principal Investigator

YOKOIGAWA Kumio  The University of Tokushima, Faculty of Integrated Arts and Sciences, Professor, 総合科学部, 教授 (60230637)

Project Period (FY) 2003 – 2006
KeywordsEscherichia coli O157 / Verocytotoxin / Spice
Research Abstract

Escherichia coli 0157 has a highly infectious ability and produces verocytotoxin that causes hemorrhagic colitis and hemolytic uremic syndrome. Since therapy for 0157 infections with antibiotics is reported to increase expression of the toxin genes, establishing an effective treatment is a priority. The purpose of this study is the establishment of attenuation of E. coli 0157 with a combination of essential oils of spices.
When we examined the effects of various spices on production of verocytotoxin by E. coli 0157, allspice showed a high suppressive effect on the verocytotoxin production. The effective component was isolated and then identified to be eugenol. Eugenol showed a suppressive effect on production of verocytotoxin 1 and 2 by the pathogen. When we examined the effects of various spices on acid- tolerance of E. coli 0157, ginger showed a high ability to reduce the acid-tolerance of the pathogen. A combination of β-pinene, limonene, eugenol, and ginger extract was highly effective on decreasing the population of the pathogen, suppressing the verocytotoxin-productivity, and reducing the acid- tolerance.

  • Research Products

    (10 results)

All 2006 2005 2004 2003

All Journal Article (10 results)

  • [Journal Article] Simple improvement in freeze-tolerance of bakers' yeast with poly-γ-glutamate.2006

    • Author(s)
      Kumio Yokoigawa
    • Journal Title

      J. Biosci. Bioeng. 102・3

      Pages: 215-219

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Simple improvement in freeze- tolerance of bakers' yeast with poly-y-glutamate.2006

    • Author(s)
      Kumio Yokoigawa
    • Journal Title

      J.Biosci.Bioeng. 102 (3)

      Pages: 215-219

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of the gene products expressed in Escherichia coli.2005

    • Author(s)
      Jiansong Ju
    • Journal Title

      J. Biosci. Bioeng. 100 (4)

      Pages: 409-417

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of the gene products expressed in Escherichia coli.2005

    • Author(s)
      Jiansong Ju
    • Journal Title

      J.Biosci.Bioeng. 100 (4)

      Pages: 409-417

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intracellular glycerol influences resistance to freeze stress in Saccharomyces cerevisiae : analysis of a quadruple mutant in glycerol-dehydrogenase genes and glycerol-enriched cells.2004

    • Author(s)
      Shingo Izawa
    • Journal Title

      Appl. Microbiol. Biotechnol. 66

      Pages: 108-114

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intracellular glycerol influences resistance to freeze stress in Saccharomyce cerevisiae : analysis of a quadruple mutant in glycerol-dehydrogenase genes and glycerol- enriched cells.2004

    • Author(s)
      Shingo Izawa
    • Journal Title

      Appl.Microbiol.Biotechnol. 66

      Pages: 108-114

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Acid Tolerance and gad mRNA Levels of Escherichia coli O157 : H7 Grown in Foods.2003

    • Author(s)
      Kumio Yokoigawa
    • Journal Title

      Int. J. Food Microbiol. 82

      Pages: 203-211

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Subunit Interaction of Monomeric Alanine Racemases from Four Shigella Species in Catalytic Reaction.2003

    • Author(s)
      Kumio Yokoigawa
    • Journal Title

      FEMS Microbiol. Lett. 221

      Pages: 263-267

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Acid Tolerance and gad mRNA Levels of Escherichia coli O157 : H7 Grown in Foods.2003

    • Author(s)
      Kumio YOKOIGAWA
    • Journal Title

      Int.J.Food Microbiol. 82

      Pages: 203-211

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Subunit Interaction of Monomeric Alanine Racemases from Four Shigella Species in Catalytic Reaction.2003

    • Author(s)
      Kumio Yokoigawa
    • Journal Title

      FEMS Microbiol.Lett. 221

      Pages: 263-267

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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