2004 Fiscal Year Final Research Report Summary
Study on a New Open Tubular Capillary Liquid Chromatography(OTCLC) System Based on Micro-Phase Separation of Mixed Solvents
| Project/Area Number |
15350046
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Saga University |
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Principal Investigator |
TABATA Masaaki Saga University, Faculty of Science and Technology, Professor, 理工学部, 教授 (40039285)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMOTO June Saga University, Instrumental Analytical Center, Associate Professor, 総合分析実験センター, 助教授 (80253582)
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| Project Period (FY) |
2003 – 2004
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| Keywords | open tubular capillary liquid chromatography / microheterogeneity / mixed solvents / salting-out / acetonitrile / ナフトール化合物の分離 / T字スプリッター / ナノパーティクル |
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| Research Abstract |
The proposed open tubular capillary liquid chromatography(OTCLC) system utilizes the recent discoveries of "microheterogeneity" in some organic-water mixed solvents and salting-out phase separation phenomena observed in these mixed solvents. For example, acetonitrile is miscible with water at any ratio ; heterogeneities, however, have been observed at a molecular level in its water mixtures. When an acetonitrile-water mixture of suitable salt concentration is pumped into such a capillary micro-phase separation is likely happening in the vicinity of the capillary wall, in a water-enriched aqueous phase attached to the capillary inner wall. In other words, a liquid membrane that has considerably different properties from the mobile phase is dynamically formed on the inner wall of the capillary.
When a mixture of analytes is injected into the capillary, participation of the analytes occurs between the mobile phase and the liquid membrane, providing the separation power of the proposed chromatograpy system.
Chromatographs clearly demonstrated the ability of separating neutral compounds from charged compounds by the OTCLC system. Moreover, it was also possible to separate compounds of similar molecular structure but different charges. The elution orders can readily be explained by the retention mechanism. p-Nitroaniline is effectively extracted to the upper (acetonitrile-enriched) phase while 2,7-NDS remains in the lower (water-enriched) phase, confirming the elution order observed. The high concentration of acetonitrile content in the mobile phase slightly increased the elution time of 1-naphthol but significantly increased the elution time of 2,6-naphtalene disulfonic acid. More interestingly, at the absence of NaCl, no separation in both cases (AN/aq 9/1 and AN/aq 8/2) were observed, demonstrating the necessity of additional electrolyte for the micro-phase separation in the capillary tubing.
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[Journal Article] Large-angle X-ray scattering, small-angle neutron Scattering, and NMR relaxation studies on mixing states of 1,4-dioxane-water, 1,3-dioxane-water, and tetrahydrofuran-water mixtures2003
Author(s)
T.Takamuku, A.Nakamizo, M.Tabata, K.Yoshida, T.Yamaguchi
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Journal Title
J.Molecular Liquds 103-1-4
Pages: 143-159
Description
「研究成果報告書概要(欧文)」より
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