2005 Fiscal Year Final Research Report Summary
Creation of extra base pairs for the development of novel functional nucleic acids
| Project/Area Number |
15350097
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIRANO Ichiro The University of Tokyo, Research Center for Advanced Science and Technology, Specially Appointed Professor, 先端科学技術研究センター, 科学技術振興特任教員(特任教授) (50173216)
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| Co-Investigator(Kenkyū-buntansha) |
MITSUI Tsuneo The University of Tokyo, Research Center for Advanced Science and Technology, Specially Appointed Associate, 先端科学技術研究センター, 科学技術振興特任教員(特任助手) (60345155)
IKEDA Shuji The University of Tokyo, Research Center for Advanced Science and Technology, Specially Appointed Associate, 先端科学技術研究センター, 科学技術振興特任教員(特任助手) (80336320)
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| Project Period (FY) |
2003 – 2005
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| Keywords | unnatural base pairs / artificial nucleic acids / artificial proteins / replication / transcription / translation |
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| Research Abstract |
The expansion of the genetic alphabet by the addition of an artificial, extra base pair allows for the site-specific, enzymatic incorporation of functional nucleotide analogs into RNA by transcription. Toward the novel biotechnology of the artificial base pair system, we have studied the creation of the extra base pairs that function in replication, transcription, and translation.
We already developed an extra base pair (s-y) that functions in transcription and translation, enabling the site-specific incorporations of nucleotide analogs into RNAs and of amino acid analogs into proteins. However, the selectivity of the s-y pair in replication is not as high as that in transcription, and the s-y pair cannot be practically used in replication, such as PCR amplification and in vivo experiments.
Here, we present several extra base pairs that exhibit unique selectivity in polymerase reactions. Among them, the Ds-Pa pair functions with extremely high complementary selectivity in replication and transcription. DNA fragments containing the Ds-Pa pair can be amplified by PCR, and both substrates of Ds and Pa can be site-specifically incorporated into RNA by T7 RNA polymerase. Thus, this extra base pair system expands the genetic alphabet, providing a novel DNA/RNA-based biotechnology for creating nucleic acids bearing extra components at desired positions.
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[Book] バイオニクス2006
Author(s)
村上 裕, 菅 裕明, 平尾 一郎
Total Pages
92
Publisher
セントラルドグマを作り変える
Description
「研究成果報告書概要(和文)」より
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[Book] RNAと創薬2006
Author(s)
平尾 一郎
Total Pages
228
Publisher
アプタマーによるシグナル伝達の阻害と制癌戦略
Description
「研究成果報告書概要(和文)」より
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[Book] ゲノムケミストリー2003
Author(s)
平尾 一郎
Total Pages
240
Publisher
ポリメラーゼ認識能をもつ人工塩基対
Description
「研究成果報告書概要(和文)」より
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