| Research Abstract |
During the project term, we clarified the essential domains for the retrotranspositions of LINE, using mainly telomere specific LINE SART1, as shown below (1 to 3, TRAS1 for 4, R1 for 5).
1. We found novel domains NLS and telomere accession signals in ORF1 proteins in SART1 (Matsumoto, T., et al.Mol.Cell Biol. 24,105-122. (2004)).
2. We found eukaryotic translational coupling for the regulation of ORF1 and ORF2 translation in SART1 (Kojima, KK.et al.Mol.Cell.Biol.25, 7675-7686. (2005)).
3. We clarified the 3'UTR structure which is recognized by reversetranscription unit of SART1 (Osanai, M., et al.Mol.Cell Biol.24, 7902-7913. (2004)).
4. Collaborating with Dr.Maita in Kyusyu Univ., we firstly reported the crystal structure of endonuclease domain of TRAS1 (Maita, N., et al.J.Biol.Chem.279, 41067-41076. (2004)).
5.We established the retrotransposion assay system for 28SrDNA specific LINE, R1, which enable us to use several target specific LINE, telomere specific LINEs, SART1 and TRAS1,and rDNA specific R1,for future analysis (Anzai T., et al.Nucleic Acids Res.33, 1993-2002. (2005)).
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