2005 Fiscal Year Final Research Report Summary
Systematic analysis of expression, function and origin of Dof transcription factors
| Project/Area Number |
15370072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo (2004-2005)
Okayama University (2003) |
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Principal Investigator |
YANAGISAWA Shuichi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20222359)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Transcription factor / Dof protein / Transcriptional regulation / Plant / Arabidopsis / Dof transcription factor |
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| Research Abstract |
Dof proteins are a group of transcription factors found only in plants. Although various physiological roles of Dof transcription factors have been suggested, roles of the majority of Dof transcription factors remain unknown. To fully understand the family of Dof transcription factors, we systematically analyzed the whole of Dof genes in Arabidopsis (a model plant). We investigated expression of all 36 Dof genes in Arabidopsis by RT-PCR. We found that most of AtDof genes are really expressed, whereas we hardly detected expression of six AtDof genes. The RT-PCR analysis with RNAs from various organs suggested organ-specific expression of several AtDof genes, although most of AtDof genes appeared to be ubiquitously and constitutively expressed. Unique expression patterns of three genes (AtDof2.3, AtDof2.4 and AtDof5.8 ) were further characterized with β-glucuronidase reporter gene in transgenic Arabidopsis plants. The analysis revealed that AtDof5.8 is expressed in young leaves, flower b
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uds and embryos. The AtDof2.3 promoter activity was preferentially observed in roots, especially in central cylinder of the upper part of roots and columella root caps. The AtDof2.4 promoter activity localized to provascular cells in seedlings and embryos. The expression pattern of AtDof2.4 was similar to that of the ATHB-8 gene, which is a member of Class III homeodomain-leucine zipper genes involved in vascular development and determination of leaf polarity. To identify novel functions of Dof transcription factors, we also performed phenotypic analysis with Arabidopsis mutants with the T-DNA insertion or the Ac/Ds transposon. We analyzed 17 out of 36 AtDof genes and found that an insertion into either of two closely related genes (AtDof3.1 and AtDof5.8) caused a similar phenotypic alteration. The disruption of AtDof3. I or AtDof5.8 led to decreased sensitivity to sugars. In addition, we found that ectopic expression of AtDof2.4 caused curling of leaves, implying involvement of AtDof2.4 in determination of leaf polarity. Less
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Research Products
(10 results)
