Global regulation of genome transcription by modulation of RNA polymerase function

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15370079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Nippon Institute for Biological Science
• Principal Investigator: ISHIHAMA Akira Nippon Institute for Biological Science, Research Division, Principal Investigator and Lab Head, 研究部, 主任研究員 (80019869)
• Co-Investigator(Kenkyū-buntansha): HONDA Ayae Nippon Institute for Biological Science, Research Division, Senior Scientist, 研究部, 研究員 (80343747) ; IWATA Akira Nippon Institute for Biological Science, Research Division, Principal Investigator, 研究部, 主任研究員 (70193745)
• Project Period (FY): 2003 – 2004
• Keywords: RNA polymease / Transcript factor / Transcription regulation / Rromoter / Microarray / SELEX / Protein phosphorylation / Transcrption signal

Research Abstract

The aim of this research was undertaken to understand the molecular basis of transcription regulation of 4,000 genes on the Escherchia coli genome. Previously we found that the total number of RNA polymerase core enzyme in E coli is only 2,000 molecules per genome. The distribution of a limited number of RNA polymerase among 4,000 genes is controlled through modulation of the promoter selectivity of RNA polymerase. For this modulation, 7 species of the sigma subunit (promoter recognition subunit) and about 300 species of transcription factors are involved. In order to reveal the molecular mechanism of RNA polymerase modulation, we have performed several lines of studies as follows :
1)A new promoter assay plasmid vector TFP (two-fluorescent protein) was constructed, into which about 1,000 E. coli promters were inserted. Using this promter eollection, growth phase-coupled variation of the promoter strength has been determined by measuring the expression of GFP/RFP ratio.
2)To identify the genes under the control of each sigma or transcription factor, the microarray assay was carried out for E. coli mutants, each lacking one specific factor, using E. coli DNA chip.
3)To identify the DNA sequences recognized by each transcription factor, an improved SELEX method was developed and applied for in vitro screening promoters recognized by each purified transcription factor.
4)The mode of transcription regulation by each transcription factor has been analyzed in vitro using the purified RNA polymerase and transcription factors and the promoters identified by microarray and SELEX.
5)Antibodies against each transcription factor were prepared and used for determination of the intracellular concentration of each sigma and transcription factor under various growth conditions.
6)NIP (nucleoid immuno-precipitation) method was developed to identify the localization in vivo of each transcription factor on the E. coli genome.
7)Kinetics and specificity of regulator phosphorylation by sensor kinase was analyzed for all two-comonent sytem proteins from E. coli. Trans-phosphorylation was observed between cognate pairs but for a limited combinations, the phosphorylation of response regular was observed by non-cognate sensor kinase, indicating the cross-talk in the activation process of transcription factor.

Research Products

• [Journal Article] Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation. (2005)
  • Author(s): Hatashi, K., Watanabe, T., Tanaka, A., Furumoto, T., Sato-Tsuchiya, C., hanaoka, F., Ohkuma, Y.
  • Journal Title: Genes Cells 10 Pages: 207-224
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The fission yeast protein, Ker1p, is an ortholog of RNA polymease I subunit A14 in Saccharomyces cerevisiae, and is required for stable association of Rrm3 and RPA21 in Poll. (2005)
  • Author(s): Imazawa, Y., Hisatake, K., Mitsuzawa, H., Matsumoto, M., Tsukui, T., Nakagawa, K., Nakadai, T., Shimada, M., Ishihama, A., Nogi, Y.
  • Journal Title: J. Biol. Chem. 280 Pages: 11467-11474
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Glyceraldehyde-3-phosphate dehydrogenase associates with RNA polymerase II and interacts with its Rpb7 subunit. (2005)
  • Author(s): Mitsuzawa, H., Kimura, M., Kanda, E., Ishihama, A.
  • Journal Title: FEBS Letters 579 Pages: 48-52
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] uvtA and pric genes involved in DNA repair and replication are negatively regulated by ArcA in Esherichia coli K12 (2005)
  • Author(s): Ogasawara, H., Teramoto, J., Hirao, K., Yamamoto, K., Ishihama, A., utsumi, R.
  • Journal Title: J. Bacteriol. (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Systematic search for the Cra-binding promoters using genomic SELEX. (2005)
  • Author(s): Shimada, T., Fujita, N., Meada M., Ishihama, A.
  • Journal Title: Genes Cells (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transcriptional response of Escherichia coli to external copper. (2005)
  • Author(s): Yamamoto, K., Ishihama, A.
  • Journal Title: Mol. Microbiol. 55 Pages: 215-227
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transcriptional response of Escherichia coli to external zinc. (2005)
  • Author(s): Yamamoto, K., Ishihama, A.
  • Journal Title: J. Bacteriol. (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cross-talks in signal transduction network among all two-component systems in Escherichia coli. (2005)
  • Author(s): Yamamoto, K., Hirano, K., Ohshima, T., Aiba, H., Utsumi, R., Ishihama, A.
  • Journal Title: J. Biol. Chem. 280 Pages: 1448-1456
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Signal transduction cascade EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli. (2005)
  • Author(s): Eguchi, Y., Okada, T., Minagawa, S., Oshima, T., Mori, H., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: J. Bacteriol. 186 Pages: 3006-3014
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High level activity of (2',3'9-oligoadenylate synthetase in dog serum.. (2005)
  • Author(s): Iwata, A., Yamamoto, A., Fujino, M., Sato, I., Hosokawa-Kanai, T., Tsuchiya, K., Ishihamra, A., Sokawa, Y.
  • Journal Title: J. Vet. Med. Sci. 66 Pages: 721-724
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation. (2005)
  • Author(s): Hayashi, K., Watanabe, T., Tanaka, A., Furumoto, T., Sato-Tsuchiya, C., Kimura, M., Yokoi, M., Ishihama, A., Hanaoka, F., Ohkuma, Y.
  • Journal Title: Genes Cells 10 Pages: 207-224
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The fission yeast protein, Kerlp, is an ortholog of RNA polymerase I subunit A14 in Saccharomyces cerevisiae, and is required for stable association of Rrm3 and RPA21 in Pol I. (2005)
  • Author(s): Imazawa, Y., Hisatake, K., Mitsuzawa, H., Matsumoto, M., Tsukui, T., Nakagawa, K., Nakadai, T., Shimada, M., Ishihama, A., Nogi, Y.
  • Journal Title: J.Biol.Chem. 280(12) Pages: 11467-11474
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptional response of Escherichia coli to external copper. (2005)
  • Author(s): Yamamoto, K., Ishihama, A.
  • Journal Title: Mol.Microbiol. 55(1) Pages: 215-227
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cross-talks in signal transduction network among all two-component systems in Escherichia coli. (2005)
  • Author(s): Yamamoto, K., Hirano, K., Ohshima, T., Aiba, H., Utsumi, R., Ishihama, A.
  • Journal Title: J.Biol.Chem. 280(2) Pages: 1448-1456
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional structural units of the Escherichia coli nucleoid revealed by atomic force microscopy. (2004)
  • Author(s): Kim, J., Yoshimura, S.H., Hizume, K., Ohniwa, R.L., Ishihama, A., Takeyasu, K.
  • Journal Title: Nucleic Acids Res. 32 Pages: 1982-1992
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Tfg3, the third subunit of general transcription factor TFIIF, functions at high temperature in the fission yeast Schizosaccharomyces pombe. (2004)
  • Author(s): Kimura, M., Ishihama, A.
  • Journal Title: Nucleic Acids Res. 32 Pages: 6706-6715
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A requlatory trade-off as a source of strain variation in the species Escherichia coli. (2004)
  • Author(s): King, T., Ishihama, A., Kori, A., Ferrencl, T.
  • Journal Title: J. Bacteriol. 186 Pages: 5614-5620
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Structure-Function Relationships of Influenza Virus RNA Polymerase : Primer-binding Site on PB1 Subunit. (2004)
  • Author(s): Koipashchikov, D., Honda, A., Ishihama, A
  • Journal Title: Biochemistry 43 Pages: 5882-5887
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcrips. (2004)
  • Author(s): Mitsuzawa, H., Kanda, E., Ishihama, A.
  • Journal Title: Nucleic Acids Res. 31 Pages: 4696-4701
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] RNA polymerase II transcription apparatus in Schizosaccharomyces pombe. (2004)
  • Author(s): Mitsuzawa, H., Ishihama, A.
  • Journal Title: Cutt. Genet 44 Pages: 287-294
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Negative regulation of the DNA repair gene (ung) epression by CpxR/cxA two-component system in Escherichia coli and induction of mutations by increased expression of CpxR. (2004)
  • Author(s): Ogasawara, H., Teramoto, J., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: J. Bacteriol. 186 Pages: 8317-8325
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Quantitative analysis of Escherichia coli promoter activity : Stationary-phase gene-associated promoters. (2004)
  • Author(s): Shimada, T., Nakinoshima, H., Ogawa, N., Maeda, M., Ishihama, A.
  • Journal Title: J. Bacteriol. 186 Pages: 7112-7122
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genome architecture studied by nano-scale imaging : Analyses among bacterial phyla and their implication to the eukaryotic genome folding. (2004)
  • Author(s): Takeyasu, K., Kim, J., Ohniwa, R., L., Kobori, T., Morikawa, K., Ohta, T., Ishihama, A., Yoshimura, S.H.
  • Journal Title: Chromosome and Genome Research 107 Pages: 38-48
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Modes of action of the TyrR protein, which result in repression and activation of the tyrP promoter of Escherichia coli. (2004)
  • Author(s): Yang, J., Hwang, S., Irawaty, W., Ishihama, A., Pittard, J.
  • Journal Title: Mol. Microbiol. 52 Pages: 243-256
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A unifying for the role of polyamines in bacterial cell growth : the polyamine modulon. (2004)
  • Author(s): Yoshida, M., Kashiwagi, K., Shigemasa, A., Shigemasa, A., Taniguchi, S., Yamamoto, K., Makinoshima, H., Ishihama, A., Igarashi, K.
  • Journal Title: J. Biol. Chem. 279 Pages: 46008-46013
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of region 4 of the sigma 70 subunit of RNA polymerase in transcription activation of the lux operon during quorum sensing. (2004)
  • Author(s): Johnson, D.C., Ishihama, A., Stevens, A.M.
  • Journal Title: FEMS Microbiol. Lett. 228 Pages: 193-201
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli. (2004)
  • Author(s): Eguchi, Y., Okada, T., Minagawa, S., Oshima, T., Mori, H., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: J.Bacteriol. 186(10) Pages: 3006-3014
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure-function relationships of inlfuenza virus RNA polymerase. (2004)
  • Author(s): Honda, A., Ishihama, A.
  • Journal Title: International Congress Series : Options for Control of Influenza Virus, V. (Y. Kawaoka, ed.) Vol.1263C Pages: 21-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High level activity of (2',3'9-oligoadenylate synthetase in dog serum. (2004)
  • Author(s): Iwata, A., Yamamoto, A., Fujino, M., Sato, I., Hosokawa-Kanai, T., Tuchiya, K., Ishihamra, A., Sokawa, Y.
  • Journal Title: J.Vet.Med.Sci. 66(6) Pages: 721-724
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy. (2004)
  • Author(s): Kim, J., Yoshimura, S.H., Hizume, K., Ohniwa, R.L., Ishihama, A., Takeyasu, K.
  • Journal Title: Nucleic Acids Res. 32(6) Pages: 1982-1992
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tfg3, the third subunit of general transcription factor TFIIF, functions at high temperature in the fission yeast Schizosaccharomyces pombe. (2004)
  • Author(s): Kimura, M., Ishihama, A.
  • Journal Title: Nucleic Acids Res. 32(22) Pages: 6706-6715
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] RNA polymerase and accessory factors. (2004)
  • Author(s): Kimura, M., Mitsuzawa, H., Ishihama, A.
  • Journal Title: The Molecular Biology of Schizosaccharomyces pombe.(Egel, R., Ed.)(Springer Pro Edit GmbH, Heidelberg.) Pages: 329-342
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A regulatory trade-off as a source of strain variation in the species Escherichia coli. (2004)
  • Author(s): King, T., Ishihama, A., Kori, A., Ferrenci, T.
  • Journal Title: J.Bacteriol. 186(17) Pages: 5614-5620
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure-Function Relationships of Influenza Virus RNA Polymerase : Primer-binding Site on PB1 Subunit. (2004)
  • Author(s): Kolpashchikov, D., Honda, A., Ishihama, A.
  • Journal Title: Biochemistry 43(19) Pages: 5882-5887
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] RNA polymerase II transcription apparatus in Schizosaccharomyces pombe. (2004)
  • Author(s): Mitsuzawa, H., Ishihama, A.
  • Journal Title: Curr.Genet. 44(6) Pages: 287-294
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Negative regulation of the DNA repair gene (ung) epression by CpxR/CpxA two-component system in Escherichia coli and induction of mutations by increased expression of CpxR. (2004)
  • Author(s): Ogasawara, H., Teramoto, J., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: J.Bacteriol. 186(24) Pages: 8317-8325
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Quantitative analysis of Escherichia coli promoter activity : Stationary-phase gene-associated promoters. (2004)
  • Author(s): Shimada, T., Makinoshima H., Ogawa, N., Maeda, M., Ishihama, A.
  • Journal Title: J.Bacteriol. 186(21) Pages: 7112-7122
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genome architecture studied by nano-scale imaging : Analyses among bacterial phyla and their implications to the eukaryotic genome folding. (2004)
  • Author(s): Takeyasu, K., Kim, J., Ohniwa, R.L., Kobori, T., Inose, Y., Morikawa, K., Ohta, T., Ishihama, A., Yoshimura, S.H.
  • Journal Title: Chromosome and Genome Research 107(1-2) Pages: 38-48
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Modes of action of the TyrR protein, which result in repression and activation of the tyrP promoter of Escherichia coli. (2004)
  • Author(s): Yang, J., Hwang, S., Camakaris, H., Irawaty, W., Ishihama, A., Pittard, J.
  • Journal Title: Mol.Microbiol. 52(1) Pages: 243-256
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A unifying model for the role of polyamines in bacterial cell growth : the polyamine modulon. (2004)
  • Author(s): Yoshida, M., Kashiwagi, K., Shigemasa, A., Shigemasa, A., Taniguchi, S., Yamamoto, K., Makinoshima, H., Ishihama, A., Igarashi, K.
  • Journal Title: J.Biol.Chem. 279(44) Pages: 46008-46013
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of a nonnatural b-C-nucleotide unit in DNA as template for DNA and RNA syntheses and as substrates for nucleolytic digestion. (2003)
  • Author(s): Aketani, S., tanaka, K., Yamamoto, K., Ishihama, A., Cao, H., Tenganji, A., Shionoya, M.
  • Journal Title: Eur. J. Pharm. Sci. 20 Pages: 43-51
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mapping sigma 54-RNA polymerase interactions at the-24 consensus promoter element. (2003)
  • Author(s): Burrows, P.C., Ishihama, A., Buck, M., Wigneshweraraj, S.R.
  • Journal Title: J. Biol. Chem. 278 Pages: 29728-29743
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transcription regulation of the drug efflux by EvgAS, a two-component system in Escherichia coli. (2003)
  • Author(s): Eguchi, Y., Oshima, T., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: Microbiology 149 Pages: 2819-2828
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In vitro properties of RpoS (ο^s) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted. (2003)
  • Author(s): Goweishankar, J., Yamamoto, K., Subbarayan, P.R., Ishihama, A.
  • Journal Title: J. Bacteriol. 185 Pages: 2673-2679
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Recruitment of s^<54>-RNA polymerase to the Pu promoter of Pseudomonas putida through integration host factor-mediated positioning switch of subunit carboxy-terminal domain on an UP-like element. (2003)
  • Author(s): Macchi, R., montesissa, L., Murakami, K., Ishihama, A., de Lorenzo, V., Bertoni, G.
  • Journal Title: J. Biol. Chem. 278 Pages: 27695-27702
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Growth phase-coupled alterations in cell structure and function of Escherichia coli. (2003)
  • Author(s): Makinoshima, H., Aizawa, S., Hayashi, H., Miki, T., Nishimura, A., Ishihama, A.
  • Journal Title: J. Bacteriol. 185 Pages: 1338-1345
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and molecular characterization of Mg (II)-regulon of Escherichia coli. (2003)
  • Author(s): Minagawa, S., Ogasawara, H., Yamamoto, K., Eguchi, T., Ishima, T., Ishihama, A., Utsumi, R.
  • Journal Title: J. Bacteriol. 185 Pages: 3696-3702
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] RNA polymerase li transcription apparatus in Schizosaccharomyces pombe. (2003)
  • Author(s): Mitsuzawa, H., Ishihama, H.
  • Journal Title: Cutt. Genet 44 Pages: 287-294
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. (2003)
  • Author(s): Mitsuzawa, H., Kanda, E., Ishihama, A.
  • Journal Title: Nucleic Acids Res. 31 Pages: 4696-4701
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The fission yeast RPA51 is a functional homolog of the budding ueast A49 subunit of RNA polymerase I and required for maximizing transcription of ribosomal RNA. (2003)
  • Author(s): Nakagawa, K., hisatake, K., Imazawa, Y., Ishiguro, A., matsumoto, M., Pape, L., Ishihama, A., Nogi, Y.
  • Journal Title: Genes Genet. Syst. 78 Pages: 199-209
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and characterization of antibacterial agents that inhibit the essential histidine kinase, YycG, from Baccilus subtilis and Staphylococcus aureus. (2003)
  • Author(s): Watanabe, T., Hashimoto, Y., Yamamoto, K., Hirao, K., Ishihama, A., Fukamizo, T., Hino, M., Utsumi, R.
  • Journal Title: J. Antibiol. 56 Pages: 1045-1052
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Two different modes of transcription repression of the Escherichia coli acetate operon by lclR. (2003)
  • Author(s): Yamamoto, K., Ishihama, A.
  • Journal Title: Mol. Microbiol 47 Pages: 183-194
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of a nonnatural b-C-nucleotide unit in DNA as template for DNA and RNA syntheses and as substrates for nucleolytic digestion. (2003)
  • Author(s): Aketani, S., Tanaka, K., Yamamoto, K., Ishihama, A., Cao, H., Tengenji, A., Shionoya, M.
  • Journal Title: Eur.J.Pharm.Sci. 20 Pages: 43-51
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mapping sigma 54-RNA polymerase interactions at the -24 consensus promoter element. (2003)
  • Author(s): Burrows, P.C., Severinov, K., Ishihama, A., Buck, M., Wigneshweraraj, S.R.
  • Journal Title: J.Biol.Chem. 278(32) Pages: 29728-29743
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcription regulation of the drug efflux genes by EvgAS, a two-component system in Escherichia coli. (2003)
  • Author(s): Eguchi, Y., Oshima, T., Mori, H., Aono, R., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: Microbiology 149(10) Pages: 2819-2828
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vitro properties of RpoS (S^S) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted. (2003)
  • Author(s): Gowrishankar, J., Yamamoto, K., Subbarayan, P.R., Ishihama, A.
  • Journal Title: J.Bacterol. 185(8) Pages: 2673-2679
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of region 4 of the sigma 70 subunit of RNA polymerase in transcription activation of the lux operon during quorum sensing. (2003)
  • Author(s): Johnson, D.C., Ishihama, A., Stevens, A.M.
  • Journal Title: FEMS Microbiol.Lett. 228(2) Pages: 193-201
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Recruitment of s^<54>-RNA polymerase to the Pu promoter of Pseudomonas putida through integration host factor-mediated positioning switch of subunit carboxy-terminal domain on an UP-like element. (2003)
  • Author(s): Macchi, R., Montesissa, L., Murakami, K., Ishihama, A., de Lorenzo, V., Bertoni, G.
  • Journal Title: J.Biol.Chem. 278(30) Pages: 27695-27702
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth phase-coupled alterations in cell structure and function of Escberichia coli. (2003)
  • Author(s): Makinoshima, H., Aizawa, S., Hayashi, H., Miki, T., Nishimura, A., Ishihama, A.
  • Journal Title: J.Bacteriol. 185(4) Pages: 1338-1345
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Principles and methods of affinity cleavages in studying transcription. (2003)
  • Author(s): Meares, C.F., Datwyler, S.A., Schmidt, B.D., Owens, J., Ishihama, A.
  • Journal Title: Meth.Enzymol. 371 Pages: 82-106
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification and molecular characterization of Mg(II)-regulon of Escherichia coli. (2003)
  • Author(s): Minagawa, S., Ogasawara, H., Kato, A., Yamamoto, K., Eguchi, T., Oshima, T., Mori, H., Ishihama, A., Utsumi, R.
  • Journal Title: J.Bacteriol. 185(13) Pages: 3696-3702
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. (2003)
  • Author(s): Mitsuzawa, H., Kanda, E., Ishihama, A.
  • Journal Title: Nucleic Acids Res. 31(16) Pages: 4696-4701
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The fission yeast RPA51 is a functional homolog of the budding yeast A49 subunit of RNA polymerase I and required for maximizing transcription of ribosomal RNA. (2003)
  • Author(s): Nakagawa, K., Hisatake, K., Imazawa, Y., Ishiguro, A., Matsumoto, M., Pape, L., Ishihama, A., Nogi, Y.
  • Journal Title: Genes Genet.Syst. 78 Pages: 199-209
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification and characterization of antibacterial agents that inhibit the essential histidine kinase, YycG, from Baccilus subtilis and Staphylococcus aureus. (2003)
  • Author(s): Watanabe, T., Hashimoto, Y., Yamamoto, K., Hirao, K., Ishiahma, A., Fukamizo, T., Hino, M., Utsumi, R.
  • Journal Title: J.Antrbiot. 56(12) Pages: 1045-1052
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Two different modes of transcription repression of the Escherichia coli acetate operon by Ic1R. (2003)
  • Author(s): Yamamoto, K., Ishihama, A.
  • Journal Title: Mol.Microbiol. 47(1) Pages: 183-194
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] uvrA and pric genes involved in DNA repair and replication are negatively regulated by ArcA in Escherichia coli K12.
  • Author(s): Ogasawara, H., Teramoto, J., Hirao, K., Yamamoto, K., Ishihama, A., Utsumi, R.
  • Journal Title: J.Bacteriol. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Systematic search for the Cra-binding promoters using genomic SELEX.
  • Author(s): Shimada, T., Fujita, N., Maeda, M., Ishihama, A.
  • Journal Title: Genes Cells (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptional response of Escherichia coli to external zinc.
  • Author(s): Yamamoto, K., Ishihama, A
  • Journal Title: J.Bacteriol. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Options for Control of Influenza Virus, V. (2004)
  • Author(s): Honda, A., Ishihama, A.
  • Total Pages: 320
  • Publisher: Elsevier Publ.
  • Description: 「研究成果報告書概要(和文)」より
• [Book] The Molecular Biology of Schizosaccharomyces pombe (2004)
  • Author(s): Kimura, M., Mitsuzawa, H., Ishihama, A.
  • Total Pages: 450
  • Publisher: Pro Edit GmbH, Heidelberg
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Meth. Enzymol. Vol. 371 (2003)
  • Author(s): Meares, C.F., Datwyler, S.A., Schmidt, B.D., Owens, J., Ishihama, A.
  • Total Pages: 350
  • Publisher: Academic Press
  • Description: 「研究成果報告書概要(和文)」より