2004 Fiscal Year Final Research Report Summary
Analyses of the transcriptional repression mechanism of TFL2,an HP1 homologue in Arabidopsis.
| Project/Area Number |
15370098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Research Institute for Biological Sciences, Okayama |
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Principal Investigator |
GOTO Koji Research Institute for Biological Sciences, Okayama, Dept of Molecular Genetics, Senior Researcher, 専門研究員(室長) (00251489)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Arabidopsis / Morphogenesis / Epigenetics / Flowering / Transcriptional regulation / Hetgerochromatin / Development / Microarray |
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| Research Abstract |
Arabidopsis terminal flower2(tfl2) mutants show photoperiod insensitive early-flowering phenotype, as well as several other pleiotropic phenotypes. We found that the early flowering of tfl2 is caused mainly by ectopic expression of the FT gene, a floral pathway integrator and that TFL2 competitively counteracts the activity of CO, an FT activator to repress ectopic FT expression. Molecular cloning of TFL2 showed that it encodes a Heterochromatin Protein 1(HP1) homolog. Gene expression analyses using DNA microarrays, however, did not show an increase in the expression of heterochromatin genes in tfl2 mutants but instead showed the upregulation of the several floral homeotic genes. This multiple genes repression by TFL2 causes the pleiotropic phenotype of the tfl2 mutant. We also examined the subcellular localization of TFL2 protein and found that it does not co-localized with the heterochromatic region of nuclei. Our results demonstrate that despite its homology to HP1,TFL2 is involved in the repression of specific euchromatin genes and not heterochromatin genes in Arabidopsis.
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Research Products
(6 results)
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[Journal Article] Functional Analysis of Isoforms of NADPH : Protochlorophyllide Oxidoreductase(POR),PORB and PORC, in Arabidopsis thaliana.2003
Author(s)
Tatsuru Masuda, Naoki Fusada, Naoki Oosawa, Kenfichi Takamatsu, Yoshiharu Y., Yamamoto, Masaaki Ohto, Kenzo Nakamuia, Koji Goto, Daisuke Shibata, Yumiko Shirano, Hiroaki Hayashi, Tomohiko Kato, Satoshi Tabata, Hiroshi Shimada, Hiroyuki Ohta, Ken-ichiro Takamiya.
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Journal Title
Plant & Cell Physiology. 44
Pages: 963-974
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Functional Analysis of Isoforms of NADPH : Protochlordphyllide Oxidoreductase (FOR), PORB and PORC, in Arabidopsis thaliana.2003
Author(s)
Tatsuru Masuda, Naoki Fusada, Naoki Oosawa, Ken-ichi Takamatsu, Yoshiharu Y.Yamamoto, Masaaki Ohio, Kenzo Nakamura, Koji Goto, Daisuke Shibata, Yumiko Shirano, Hiroaki Hayashi, Tomohiko Kato, Satoshi Tabata, Hiroshi Shimada, Hiroyuki Ohta, Ken-ichiro Takamiya.
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Journal Title
Plant Cell Physiol. 44
Pages: 963-974
Description
「研究成果報告書概要(欧文)」より
