2006 Fiscal Year Final Research Report Summary
Molecular and physiological basis of Rubisco turnover and its relation to nitrogen nutrition
| Project/Area Number |
15380046
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Tohoku University |
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Principal Investigator |
MAE Tadahiko Tohoku University, Graduate School of Agricultural Science, Professor, 大学院農学研究科, 教授 (60134029)
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| Co-Investigator(Kenkyū-buntansha) |
MAKINO Amane Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院農学研究科, 助教授 (70181617)
ISHIDA Hiroyuki Tohoku University, Graduate School of Agricultural Science Tohoku University, Assistant Professor, 大学院農学研究科, 助手 (60312625)
SUZUKI Yuji Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院農学研究科, 助手 (80374974)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Rubisco / turnover / synthesis・degradation / rbcS / rbcL / Rubisco-containing body (RCB) / reactive-oxygen species / fragmentation |
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| Research Abstract |
Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of Rubisco synthesized in rice leaves from emergence through senescence.
The levels of rbcS and rbcL mRNAs, the amount of Rubisco synthesized, and the N influx greater for the 4 mM treatment than for the 1 mM treatment throughout the experiment. The mount of Rubisco synthesized was well correlated with the levels of both mRNAs during leaf expansion, but not after the completion of leaf expansion in both N treatments. The ratio of the amount of Rubisco synthesized to the levels of both mRNAs dramatically declined after full expansion. On the other hand, the amount of Rubisco synthesized was well correlated with the N influx in both N treatments. These results indicate that the N influx, namely, N availability, rather than the levels of rbcS and rbcL mRNAs, is more closely related to the amount of Rubisco synthesized in the leaf blade of rice throughout the life span of a leaf.
Exclu
… More
sion of Rubisco from chloroplasts by specific bodies in naturally senescing leaves of wheat
Immunocytochemical electron-microscopic observation indicated that Rubisco and/or its degradation products are localized in small spherical bodies having a diameter of 0.4-1.2 μm in naturally senescing leaves of wheat. These Rubisco containing bodies (RCBS) were found in the cytoplasm and in the vacuoles,. RCBs contained another stromal protein, chloroplastic glutamine synthetase, but not thylaloid proteins. Ultrastructural analysis suggested that RCBs had double membranes, which seemed to be derived from the chloroplast envelop, and that RCBS were further surrounded by the other membrane structures in the cytoplasm. The appearance of RCBs was the most remarkable when the amount of Rubisco started to decrease at the early phase of leaf senescence. These results suggest that RCBS might be involved in the degradation process of Rubisco out side of chloroplasts during leaf senescence.
In vivo frgmentation of the large subunit of Rubisco by reactive oxygen species in an intact leaf of Cucumber under chilling conditions.
When lea discs of chilling-sensitive cucumber were illuminated at 4-℃, five major fragments of the LSU were observed. This fragmentation was completely inhibited by ROS scavengers and Tiron stimulated this fragmentation, whereas an iron-specific chelator, deforoxamine, suppressed it. Further, such fragments were identical to those generated from the purified Rubisco by an-OH-generating system in vitro on two-dimensional PAGE. These results indicate that the direct fragmentation of Rubisco by reactive oxygen species also occurs in an intact leaf. Less
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Research Products
(39 results)
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[Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rvcS gene.2007
Author(s)
Suzuki, Y., Ohkubo, M., Hatakeyama, H., Ohashi, K., Yoshizawa, R., Kojima, S., Hayakawa, T., Yamaya, T., Mae, T., Makino, A.
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Journal Title
Plant Cell Physiol. 48 (4)
Pages: 626-637
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A. A large-grain cultivar, Akita 63, exhibits high yields with high physiological N-use efficiency.2006
Author(s)
Mae, T., Inaba, A., Kaneta, Y., Masaki, S., Sasaki, M., et al.
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Journal Title
Field Crops Res. 97
Pages: 227-237
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A large-grain cultivar, Akita 63, exhibits high yields with high physiological N-use efficiency.2006
Author(s)
Mae, T., Inaba, A., Kaneta, Y., Masaki, S., Sasaki, M., Aizawa, M., Okawa, S., Hasegawa, S., Makino, A.
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Journal Title
Field Crops Res. 97
Pages: 227-237
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Rice is life : Scientific Perspectives for the 21st Century.2005
Author(s)
Mae, T., Inaba, A., Kaneta, Y., Masaki, S., Sasaki, M., Makino, A.
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Journal Title
Proceedings of the World Rice Research Conference, Tsukuba, Japan
Pages: 590
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Exclusion of ribulose-1,5-bisphosphate carboxylase/oxygenase from chloroplasts by specific bodies in naturally senescing leaves of wheat.2003
Author(s)
Chiba, A., Ishida, H., Nishizawa, N., Makino, A., Mae, T.
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Journal Title
Plant Cell Physiol. 44・9
Pages: 914-921
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Xanthophyll cycle pigments and water-water cycle in transgenic rice with decreased amounts of ribulose-1,5-bisphosphate carboxylae and the wild-type rice grown under different N levels.2003
Author(s)
Ushio, A., Makino, A., Yokota, S., Hirotsu, N., Mae, T.
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Journal Title
Soil Sci. Plant Nutr. 49・1
Pages: 77-83
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Exclusion of ribulose-1,5-bisphosphate carboxylase/oxygenase from chloroplasts by specific bodies in naturally senescing leaves of wheat.2003
Author(s)
Chiba, A., Ishida, H., Nishizawa, N., Makino, A., Mae, T.
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Journal Title
Plant Cell Physiol. 44 (9)
Pages: 914-921
Description
「研究成果報告書概要(欧文)」より
-
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[Journal Article] Xanthophyll cycle pigments and water-water cycle in transgenic rice with decreased amounts of ribulose-1,5-bisphosphate carboxylae and the wild-type rice grown under different N levels.2003
Author(s)
Ushio, A., Makino, A., Yokota, S., Hirotsu, N., Mae, T.
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Journal Title
Soil Sci.Plant Nutr. 49 (1)
Pages: 77-83
Description
「研究成果報告書概要(欧文)」より
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[Book] Rice is life : Scientific Perspectives for the 21st Century2005
Author(s)
Mae, T., Inaba, A., Kaneta, Y., Masaki, S., Sasaki, M., Makino, A.
Total Pages
590
Publisher
Proceedings of the World Rice Research Conference, Tsukuba, Japan
Description
「研究成果報告書概要(和文)」より
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[Book] 生物化学2005
Author(s)
牧野 周, 前 忠彦
Total Pages
279
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
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[Book] Biochemistry2005
Author(s)
Makino, A., Mae, T.
Total Pages
279
Publisher
Asakura Syoten
Description
「研究成果報告書概要(欧文)」より
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