2005 Fiscal Year Final Research Report Summary
Elucidation of the Interaction between Nutrition and the Gene Expression Control System Based on "Omics" Analyses
| Project/Area Number |
15380090
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KATO Hisanori The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (40211164)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIZAWA Fumiaki Utsunomiya University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10269243)
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| Project Period (FY) |
2003 – 2005
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| Keywords | transcriptomics / nutrigenomics / proteomics / amino acids / dietary protein / food safety / transcription factor / database |
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| Research Abstract |
To extend the transcriptomic analysis of the effect of dietary protein, the effect of protein nutrition on the gene expression profile of adipose tissue was examined. The up-regulation of the PPARγ gene was thought to be involved in the elevated sensitivity to insulin under protein malnutrition. Additionally, function analysis of some animal proteins was carried out by using a DNA microarray. Then the effect of amino acid deprivation on transcriptome and proteome of a hepatoma cell line was analyzed. Many genes were found to directly respond to amino acids; they included the genes for some transcription factors and translation factors. We then explored the application of omics approach to the toxic effect of the excess of an amino acid. The result indicated that, in the case of cystine excess, the liver and heart are major targets of its toxic effect. The regulatory mechanisms of transcription and translation by amino acids were then examined in detail. Transcription factors such as Foxos and ATFs turned out to play important roles. The mechanism whereby amino acids regulate the signaling pathway of insulin was studied in light of a mediating factor, IRS-1. We could propose a novel regulatory mechanism of cell signaling in which amino acids serves as regulators of some phosphatases.
Moreover, to explore a new direction of omics analyses in nutrigenomics area, we attempted to use transcriptomics for the evaluation of the safety of food. The safety of hypoallergenic wheat flour was confirmed with this technique, showing the effectiveness of this approach. It seemed to be essential to build a nutrigenomics database for efficient development of nutrigenomics area. A unique database was created and publicized. Raw data of DNA microarray analyses and publication information on nutrigenomics experiments are being accumulated.
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Research Products
(31 results)
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[Journal Article] Safety evaluation of a hypoallergenic wheat flour using DNA microarray.2006
Author(s)
Narasaka, S., Endo, Y., Fu, ZW., Moriyama, M., Arai, S., Abe, K., Kato, H.
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Journal Title
Biosci. Biotechnol. Biochem. 70・6(印刷中)
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Biosci. Biotechnol. Biochem.2005
Author(s)
Hirao, T., Imai, S., Sawada, H., Shiomi, N., Hachimura, S., Kato, H.
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Journal Title
PCR method for detecting trace amounts of buckwheat (Fagopyrum spp.) in food. 69・4
Pages: 724-731
Description
「研究成果報告書概要(和文)」より
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[Journal Article] J. Agric. Food Chem.2005
Author(s)
Tachibana, N., Matsumoto, I., Fukui, K., Arai, S., Kato, H., Abe, K.
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Journal Title
Intake of soy protein isolate alters hepatic gene expression in rats. 53・10
Pages: 4253-4257
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Transcriptomics and metabolomics of diertary leucine excess.2005
Author(s)
Matsuzaki, K., Kato, H., Sakai, R., Toue, S., Amao, M., Kimura, T.
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Journal Title
J. Nutr. 135・6
Pages: 1571-1575
Description
「研究成果報告書概要(和文)」より
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[Journal Article] PCR method for detecting trace amounts of buckwheat (Fagopyrum spp.) in food.2005
Author(s)
Hirao, T., Imai, S., Sawada, H., Shiomi, N., Hachimura, S., Kato, H.
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Journal Title
Biosci.Biotechnol.Biochem. 69-4
Pages: 724-731
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intake of soy protein isolate alters hepatic gene expression in rats.2005
Author(s)
Tachibana, N., Matsumoto, I., Fukui, K., Arai, S., Kato, H., Abe, K.
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Journal Title
J.Agric.Food Chem. 53-10
Pages: 4253-4257
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transcriptomics and metabolomics of dietary leucine excess.2005
Author(s)
Matsuzaki, K., Kato, H., Sakai, R., Toue, S., Amao, M., Kimura, T.
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Journal Title
J.Nutr. 135-6
Pages: 1571-1575
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] J. Mol. Endocrinol.2004
Author(s)
Toyoshima, Y., Ohne, Y., Takahashi, S.-I., Noguchi, T., Kato, H.
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Journal Title
Dietary protein deprivation decreases the serine phosphorylation of insulin receptor substrate-1 in rat skeletal muscle. 32・2
Pages: 519-531
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Briofactors2004
Author(s)
Kato, H., Narasaka, S., Endo, Y., Abe, K., Arai, S.
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Journal Title
DNA microarray analyses of the effects of dietary proteins. 21・1
Pages: 11-13
Description
「研究成果報告書概要(和文)」より
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[Journal Article] J. Nutr.2003
Author(s)
Kato, H., Kimura, T.
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Journal Title
Evaluation of the effects of the dietary intake of proteins and amino acids by DNA microarray technology. 133・6
Pages: 2073S-2077S
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Safety evaluation of a hypoallergenic wheat flour using DNA microarray.
Author(s)
Narasaka, S., Endo, Y., Fu, ZW., Moriyama, M.Arai, S., Abe, K., Kato, H.
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Journal Title
Biosci.Biotechnol.Biochem. 70-6 (in press)
Description
「研究成果報告書概要(欧文)」より
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