2006 Fiscal Year Final Research Report Summary
Development of a diagnostic system for definitive hosts of taeniid cestodes and an epizootiological survey for echinococcosis in urban area
| Project/Area Number |
15380205
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
NONAKA Nariaki Hokkaido University, Graduate School of Veterinary Medicine, Lecturer, 大学院獣医学研究科, 講師 (50281853)
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| Co-Investigator(Kenkyū-buntansha) |
OKU Yuzaburo Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院獣医学研究科, 助教授 (60133716)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Echinococcosis / Diagnosis / Dog / Fox / PCR / Coproantigen / DNA / Deworming |
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| Research Abstract |
Echinococcus multilocularis is a zoonotic parasite endemic in Hokkaido. The definitive host of the parasite carnivore animal such as fox and dog, and the intermediate host is rodent. Humans get infection by accidentally ingestin; the parasite eggs. The prevalence in foxes in Hokkaido was about 40% in the last decade and that in pet dogs is 0.3-0.7% In response to a deteriorating situation, reporting system for canine echinococcosis has been enforced since 2004 in Japan. This study has aimed to improve a diagnostic system for the definitive hosts by incorporating specific DNA detection and t, investigate an urban life cycle of the parasite and risk for infection in Sapporo and its suburbs. In research for diagnosti, system, PCR with specific primers for E. multilocularis and PCR-RFLP for other taeniid cestodes were developed for the egg of taeniid cestodes that were found in fecal egg examination but morphologically indistinguishable among the family. Moreover DNA diagnosis in prepatent period in which eggs are not excreted in host feces was shown to be feasible by examining th, post-dewormed feces. In addition, high reliability of a developing coproantigen detection kit which can be incorporated i the diagnostic system was shown using the samples collected in Japan and other countries. During the evaluation, a cat excretin E. multilocularis eggs were found at first in Japan. In investigation on an urban life cycle, high prevalence was found i foxes inhabiting in Sapporo and Otaru. Foxes showed geographical preference for selecting their den sites. Therefore, potentia home range of infected foxes, thus a risk area for Echinococcus infection in the urban area could be estimated.
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[Journal Article] Echinococcosis risk among domestic definitive hosts, Japan2007
Author(s)
Kamiya, M., Lagapa, J.T., Ganzorig, S., Kobayashi, F., Nonaka, N., Oku, Y.
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Journal Title
Emerging Infectious Diseases 13(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Epizootiological survey of Trichinella spp. infection in carnivores, rodents and insectivores in Hokkaido, Japan2007
Author(s)
Kanai, Y., Inoue, T., Mano, T., Nonaka, N., Katakura, K., Oku, Y.
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Journal Title
Japanese Journal of Veterinary Research 54(4)
Pages: 175-182
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of animals excreting feces collected in field for epizootiological studies of echinococcosis2006
Author(s)
Nonaka, N., Inoue, T., Sano, T., Katakura, K., Fukui, D., Oku, Y.
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Journal Title
Japanese Journal of Veterinary Parasitology 4(2)
Pages: 38
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Survey of Coccidial Oocysts and Parasite Eggs in Feces of Free-Ranging Grus ianonensis2006
Author(s)
sarashina, T., Uzuka, Y Tananabe, S., OKu, Y Watanabe, Y., Kurosawa, N., Nishimura, M.
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Journal Title
Japanese Journal of Veterinary Sciences 68(8)
Pages: 873-875
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Parasitic helminths from feral raccoons (Procyon lotor) in Japan2006
Author(s)
Matoba, Y., Yamada, D., Asano, M., Oku, Y., Kitaura, K., Yagi, K., Tenora F., Asakawa, M.
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Journal Title
Helminthologia 43(3)
Pages: 139-146
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Current control strategies targeting sources of echinococcosis in Japan2006
Author(s)
Kamiya, M., Lagapa, J.T.G., Nonaka, N., Ganzorig, S., Oku, Y., Kamiya, H.
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Journal Title
Revue Scientifique et Technique de l'Office International des Epizooties 25(3)
Pages: 1055-1066
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Production and characterization of monoclonal antibodies against excretory/secretory products of adult Echinococcus granulosus, and their application to coproantigen detection2005
Author(s)
Casaravilla, C., Malgor, R., Rossi, A., Sakai, H., Nonaka, N., Kamiya, M., Carmona, C.
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Journal Title
Parasitology International 54(1)
Pages: 43-49
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Development of Taenia saginata asiatica metacestodes in SCID mice and its infectivity in human and alternative definitive hosts2005
Author(s)
Chang, S.L., Nonaka, N., Kamiya, M., Kanai, Y., Ooi, H.K., Chung, W.C., Oku, Y.
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Journal Title
Parasitology Research 96(2)
Pages: 95-101
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A servey of gastrointestinal parasites in dogs and cats in Kanto district2005
Author(s)
Konno, K., Hata, H., Nonaka, N., Oku, Y., Ito, T., Sakai, T., Kamiya, M.
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Journal Title
Japanese Journal of Veterinary Parasitology 3(2)
Pages: 47
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Survey on companion animals for Echinococcus multilocularis infection (1997-2002)2003
Author(s)
Kamiya, M., Nonaka, N., Oku, Y., Ando, Y., Tachibana, T., Tamai, S.
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Journal Title
Japanese Journal of Veterinary Parasitology 2(1)
Pages: 38
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Control measure against the definitive hosts of Echinococcus multilocularis in Hokkaido2003
Author(s)
Oku, Y., Iwaki, T., Nonaka, N., Kanai, Y., Mizuno, A., Kamiya, M.
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Journal Title
Japanese Journal of Veterinary Parasitology 2(1)
Pages: 39
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Control of Echinococcus multilocularis infection in the definitive hosts at the outskirts of urban area : a mass-deworming trial of wild foxes in Otaru, Hokkaido, Japan2003
Author(s)
Inoue, T., Ode, T., Kanai, Y., Iwaki, T., Mizuno, A., Nonaka, N., Oku, Y., Kamiya, M.
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Journal Title
Japanese Journal of Veterinary Parasitology 2(1)
Pages: 40
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Possibility of invasion of Echinococcus into Honshu with pet dogs from Hokkaido and overseas2003
Author(s)
Doi, R., Matsuda, H., Uchida, A., Kanda, E., Kamiya, H., Konno, K., Tamashiro, H., Nonaka, N., Oku, Y., Kamiya, M.
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Journal Title
Japanese Journal of Public Health 50(7)
Pages: 639-649
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Biology of Echinococcus2003
Author(s)
Oku, Y., Kamiya, M.
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Journal Title
Progress of Medical Parasitology in Japan, Volume 8, Chapter III. M. Otsuru, S. KAMEGAI and S. Hayashi eds. Tokyo, Meguro Parasitological Museum
Pages: 294-318
Description
「研究成果報告書概要(欧文)」より
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[Book] 人獣共通感染症2004
Author(s)
野中成晃
Total Pages
447
Publisher
医薬ジャーナル社
Description
「研究成果報告書概要(和文)」より
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[Book] 人獣共通感染症2004
Author(s)
奥祐三郎
Total Pages
447
Publisher
医薬ジャーナル社
Description
「研究成果報告書概要(和文)」より
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