2005 Fiscal Year Final Research Report Summary
Molecular mechanism of L-glutamate and GABA-mediated regulation of secretion of glucagon and insulin in islets of Langerhans
| Project/Area Number |
15390026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
MORIYAMA Yoshinori OKAYAMA UNIVERSITY, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Professor, 大学院・医歯薬学総合研究科, 教授 (10150658)
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| Co-Investigator(Kenkyū-buntansha) |
OTUSKA Masato OKAYAMA UNIVERSITY, Advanced Science Research Center, assistant, 自然生命科学研究支援センター, 助手 (30243489)
YAMADA Hiroshi OKAYAMA UNIVERSITY, Graduate School of Medicine, Dentistry and Pharmaceutical Science, assistant, 大学院・医歯薬学総合研究科, 助手 (80325092)
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| Project Period (FY) |
2003 – 2005
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| Keywords | B cell / A cell / islet of Langerhans / vesicular glutamate transporter / vesicular GABA transporter / glutamate / GABA / 小胞型グルタミン酸トランスポーター |
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| Research Abstract |
Vesicular glutamate transporter (VGLUT) and vesicular GABA transporter (VGAT) play essential roles in the glutamatergic and GABAergic chemical transduction, respectively. VGLUT and VGAT are present not only in the neuronal synaptic vesicles but also in the glucagon-containing secretory granules of A cells of islets of Langerhans, and are responsible for vesicular storage of glutamate and GABA, respectively. This research aimed to reveal the molecular mechanism of the glutamate- and GABA-mediated regulation of secretion of glucagon and insulin in the islets. During three years, we have obtained the following important results.
(1)We have revealed topology of VGLUT2.
(2)We have established an in vitro assay system for VGLUT, and identified some essential amino acid residues for transport of glutamate as well as targeting.
(3)We have established an in vitro assay system for VGAT.
(4)We have observed decreased glutamatergic signaling in VGLUT1 KO mice.
(5)We have identified and characterized glutamate signaling pathway in intestinal L cells.
(6)We have identified and characterized a series of receptors for regulation of secretion of insulin and glucagon.
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Research Products
(38 results)
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[Journal Article] Vesicular storage and secretion of L-glutamate from glucagon-like peptide 1-secreting clonal intestinal L cells.2006
Author(s)
Uehara, S., Jung, S.K., Morimoto, R., Arioka.S., Miyaji, T., Juge, N., Hiasa, M., Shimizu, K., Ishimura, A., Otsuka, M., Yamamoto, A., Maechler, P., Moriyama Y.
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Journal Title
J. Neurochem. 96
Pages: 550-560
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Vesicular storage and secretion of L-glutamate from glucagon-like peptide 1-secreting clonal intestinal L cells.2006
Author(s)
Uehara, S., Jung, S.K., Morimoto, R., Arioka, S., Miyaji, T., Juge, N., Hiasa, M., Shimizu, K., Ishimura, A., Otsuka, Yamamoto, A., Maechler, P., Moriyama, Y.
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Journal Title
J.Neurochem. 96
Pages: 550-560
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of essential amino acid residues of the NorM Na^+/multidrug antiporter in Vibrio parahaemolyticus.2005
Author(s)
Otsuka, M., Yasuda, M., Morita, Y., Otsuka, C., Tsuchiya, T., Omote.H., Moriyama, Y.
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Journal Title
J. Bacteriol. 187
Pages: 1552-1558
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A human transpoter protein that mediates the final excretion step for toxic organic cations.2005
Author(s)
Otsuka, M., Matsumoto, T., Morimoto, R., Arioka, S., Omote, H., Moriyama, Y.
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Journal Title
Proc. Natl. Acad. Sci. USA 102
Pages: 17923-17928
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Proteolipid of vacuolar H^+-ATPase of Plasmodium falciparum : cDNA cloning, gene organization and complementation of a yeast null mutant.2005
Author(s)
Yatsushiro, S., Taniguchi, S., Mitamura, T., Omote, H., Moriyama, Y.
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Journal Title
Biochim. Biophy. Acta 1717
Pages: 89-96
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification of essential amino acid residues of the NorM Na^+/multidrug antiporter in Vibrio parahaemolyticus.2005
Author(s)
Otsuka, M., Yasuda, M., Morita, Y., Otsuka, C., Tsuchiya, T., Omote, H., Moriyama, Y.
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Journal Title
J.Bacteriol. 187
Pages: 1552-1558
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A human transpoter protein that mediates the final excretion step for toxic organic cations.2005
Author(s)
Otsuka, M., Matsumoto, T., Morimoto, R., Arioka, S., Omote, H., Moriyama, Y.
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Journal Title
Proc.Natl.Acad.Sci.USA 102
Pages: 17923-17928
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Proteolipid of vacuolar H^+-ATPase of Plasmodium falciparum : cDNA cloning, gene organization and complementation of a yeast null mutant.2005
Author(s)
Yatsushiro, S., Taniguchi, S., itamura, T., Omote, H., Moriyama, Y.
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Journal Title
Biochim.Biophys.Acta. 1717
Pages: 89-96
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Overexpression of constitutively activated glutamate dehydrogenase induces insulin secretion through enhanced glutamate oxidation.2004
Author(s)
Anno, T., Uehara, S., Katagiri, H., Ohta, Y., Ueda, K., Mizuguchi, H., Moriyama, Y., Oka, Y., Tanizawa Y.
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Journal Title
Am. J. Physiol 286
Pages: E280-E285
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Metabotropic glutamate receptor type 4 is involved in auto-inhibitory cascade for glucagon secretion by α cells of islet of Langerhans.2004
Author(s)
Uehara, S., Muroyama, A., Echigo, N., Morimoto, R., Otsuka, M., Yatsushiro, S., Moriyama, Y.
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Journal Title
Diabetes 53
Pages: 998-1006
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A novel variant of ionotropic glutamate receptor regulates somatostatin secretion from δ-cells of islets of Langerhans.2004
Author(s)
Muroyama, A., Uehara.S., Yatsushiro, S., Echigo, N., Morimoto, R., Morita, M., Hayashi, M., Yamamoto, A., Koh, D.S., Moriyama, Y.
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Journal Title
Diabetes 53
Pages: 1743-1753
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature.2004
Author(s)
Yoshida, Y., Kinuta, M., Abe, T., T.Liang, S., Araki, K., Cremona, O., Paolo, G.D., Moriyama, Y., Yasuda, T., Camilli, P.D., Takei, k.
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Journal Title
EMBO. J. 23
Pages: 3843-3941
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Overexpression of constitutively activated glutamate dehydrogenase induces insulin secretion through enhanced glutamate oxidation.2004
Author(s)
Anno, T., Uehara, S.Katagiri, H., Ohta, Y., Ueda, K., Mizuguchi, H., Moriyama, Y., Oka, Y., Tanizawa, Y.
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Journal Title
Am.J.Physiol. 286
Pages: E280-E285
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Metabotropic glutamate receptor type 4 is involved in auto-inhibitory cascade for glucagon secretion by α cells of islet of Langerhans.2004
Author(s)
Uehara, S., Muroyama, A., Echigo, N., Morimoto, R., Otsuka, M., Yatsushiro, S., Moriyma, Y.
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Journal Title
Diabetes 53
Pages: 998-1006
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel variant of ionotropic glutamate receptor regulates somatostatin secretion from δ-cells of islets of Langerhans.2004
Author(s)
Muroyama, A., Uehara, S., Yatsushiro, S., Echigo, N., Morimoto, R., Morita, M., Hayashi, M., Yamamoto, A., Koh, D.S., Moriyama, Y.
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Journal Title
Diabetes 53
Pages: 1743-1753
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature.2004
Author(s)
Yoshida, Y., Kinuta, M., Abe, T., Liang, S., Araki, K., Cremona, O., Paolo, G.D., Moriyama, Y., Yasuda, T., Camilli, P.D., Takei, K.
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Journal Title
EMBO.J. 23
Pages: 3483-3491
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Vesicular inhibitory amino acid transporter is present in glucagon-containing secretory granules in α TC6 cells, mouse clonal α cells, and α cells of islets of Langerhans.2003
Author(s)
Hayashi, M., Otsuka, M., Morimoto, R., Muroyama, A., Uehara, S., Yamamoto, A., Moriyama, Y.
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Journal Title
Diabetes 52
Pages: 2066-2074
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Generation of an erythrocyte protein secretory system by Plasmodium falciparum Malaria parasites.2003
Author(s)
Tarashi, T.F., O'Donnell, M., Martinez, S., Schneider, T., Trelka, D., Tilley, L., Moriyama, Y.
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Journal Title
Blood 102
Pages: 3420-3426
Description
「研究成果報告書概要(欧文)」より
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