2006 Fiscal Year Final Research Report Summary
Cytogenetic and molecular pathologic profiles of bone and soft tissue tumors, and their MMPs expression
| Project/Area Number |
15390119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Fukuoka University |
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Principal Investigator |
IWASAKI Hiroshi Fukuoka University, School of Medicine, Professor, 医学部, 教授 (90101170)
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| Co-Investigator(Kenkyū-buntansha) |
NABESHIMA Kazuki Fukuoka University, School of Medicine, Professor, 医学部, 教授 (40189189)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Bone and soft tissue tumors / molecular genetics / chromosomal abnormalities / matrix metalloproteinases (MMPs) / sarcoma- / emmprin / malignant peripheral nerve sheath / solid tumors tumors |
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| Research Abstract |
1) A new human cell line, FU-DDLS-1 established from a dedifferentiated liposarcoma exhibited immunopositive reaction for mdm2 and p53 proteins. Cytogenetically, FU-DDLS-1 displayed a hypertetraploid karyotype with giant marker chromosomes composed partly of chromosome 12 materials. CGH analysis demonstrated that a gain of 12q12-q21 detected in FU-DDLS-1 were essentially the same as those in the original sarcoma. The FU-DDLS-1 cell line may be a particularly useful model for studying the molecular pathogenesis of human dedifferentiated liposarcoma.
2) cDNA microarray analysis of malignant peripheral nerve sheath tumors (MPNST) : Survivin and tenascin C expressions were upregulated in MPNST, validated by reverse transcription polymerase chain reaction. Immunohistochemistry confirmed upregulation of survivin in MPNST at the protein level in six of eight cases compared with benign tumours. Tenascin C was also expressed at the invasive front and tumorous stroma in all MPNST cases. Survivin
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and tenascin C may be associated with the malignant potential of MPNST and could be considered as potential therapeutic targets.
3) Expression of emmprin and matrix metalloproteinases (MMPs) in peripheral nerve sheath tumors (PNSTs) : We found that emmprin and MT1-MMP may be malignant potential-related proteins in PNSTs, and that MMP-1 and 9 may help differentiation between schwannoma and neurofibroma, especially in their plexiform types. The expression patterns of MMP-1 and gelatinase B (MMP-9) could divide PNSTs into two groups : schwannoma versus neurofibroma/malignant PNST (MPNST). Higher expression levels (>3+) of MMP-9 were observed in 50% of schwannomas versus none in neurofibromas and MPNSTs, while those of MMP-1 were found in 35.7% of neurofibromas and 66.7% of MPNSTs versus none in schwannomas. RECK was the main inhibitor expressed in these 3 tumors, with no significant differences.
4) Epithelioid sarcoma (ES) cell lines showed that they express emmprin, and co-culture of these ES cells with dermal fibroblasts resulted in upregulation of gelatinase A (MMP-2) in fibroblasts. This stimulation was inhibited by an activity-blocking peptide against emmprin and by antiemmprin antibody. Immunohistochemical analysis of 5 ES patient cases demonstrated diffuse emmprin expression in ES cells and MMP-2 expression in both ES cells and peritumoral fibroblasts. Soluble full-length emmprin released from ES cells was shown to stimulate MMP-2 production by fibroblasts. In conclusion, emmprin is expressed in ES in both membrane and soluble forms and stimulates MMP-2 production via interactions with fibroblasts, which could play a role in ES cell stromal invasion and vascular involvement. Less
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Research Products
(81 results)
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[Journal Article] Establishment of three malignant peripheral nerve sheath tumor cell lines, FU-SFT8611, 8710 and 9817 : conventional and molecular cytogenetic characterization.2006
Author(s)
Aoki M, Nabeshima K, Nishio J, Ishiguro M, Fujita C, Koga K, Hamasaki M, Kaneko Y, Iwasaki H
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Journal Title
Int J Oncol 29
Pages: 1421-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prognostic significance of dysadherin expression in epithelioid sarcoma and its diagnostic utility in distinguishing epithelioid sarcoma from malignant rhabdoid tumor.2006
Author(s)
Izumi T, Oda Y, Hasegawa T, Nakanishi Y, Iwasaki H, Sonobe H, Goto H, Kusakabe H, Takahira T, Kobayashi C, Kawaguchi K, Saito T, Yamamoto H, Tamiya S, Iwamoto Y, Tsuneyoshi M
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Journal Title
Mod Pathol 19
Pages: 820-831
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Establishment and characterization of a novel myxofibrosarcoma cell line2005
Author(s)
Kawashima H, Ogose A, Gu W, Nishio J, Kudo N, Kondo N, Hotta T, Umezu H, Tohyama T, Nishijima H, Iwasaki H, Endo N
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Journal Title
Cancer Genet Cytogenet 161
Pages: 28-35
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Intraductal tubular adenoma of the pancreas, pyloric gland type : a clinicopathologic and immunohistochemical study of 6 cases2005
Author(s)
Nakayama Y, Inoue H, Hamada Y, Takeshita M, Iwasaki H, Maeshiro K, Iwanaga S, Tani H, Ryu S, Yasunami Y, Ikeda S
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Journal Title
Am J Surg Pathol 29
Pages: 607-616
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Establishment of a new human epithelioid sarcoma cell line, FU-EPS-1 : molecular cytogenetic characterization by use of spectral karyotyping and comparative genomic hybridization.2005
Author(s)
Nishio J, Iwasaki H, Nabeshima K, Ishiguro M, Naumann S, Isayama T, Naito M, Kaneko Y, Kikuchi M, Bridge JA
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Journal Title
Int J Oncol 27
Pages: 361-369
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of a ring chromosome with spectral karyotyping in a pleural synovial sarcoma.2005
Author(s)
Nishio J, Iwasaki H, Althof PA, Naumann S, Ishiguro M, Haraoka S, Iwashita A, Iwasaki A, Kaku Y, Kaneko Y, Kikuchi M, Bridge JA
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Journal Title
Cancer Genet Cytogenet 160
Pages: 174-178
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Establishment and characterization of a novel myxofibrosarcoma cell line.2005
Author(s)
Kawashima H, Ogose A, Gu W, Nishio J, Kudo N, Kondo N, Hotta T, Umezu H, Tohyama T, Nishijima H, Iwasaki H, Endo N
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Journal Title
Cancer Genet Cytogenet 161
Pages: 28-35
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intraductal tubular adenoma of the pancreas, pyloric gland type : a clinicopathologic and immunohistochemical study of 6 cases.2005
Author(s)
Nakayama Y, Inoue H, Hamada Y, Takeshita M, Iwasaki H, Maeshiro K, Iwanaga S, Tani H, Ryu S, Yasunami Y, Ikeda S
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Journal Title
Am J Surg Pathol 29
Pages: 607-616
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Localization of the VEGF and angiopoietin genes in uterine carcinosarcoma.2004
Author(s)
Emoto M, Charnock-Jones DS, Licence DR, Ishiguro M, Kawai M, Yanaihara A, Saito T, Hachisuga T, Iwasaki H, Kawarabayashi T, Smith SK
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Journal Title
Gynecol Oncol 95
Pages: 474-482
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A tetraspanin-family protein, T-cell acute lymphoblastic leukemia-associated antigen 1, is induced by the Ewing's sarcoma-Wilms' tumor 1 fusion protein of desmoplastic small round-cell tumor2003
Author(s)
Ito E, Honma R, Imai J, Azuma S, Kanno T, Mori S, Yoshie O, Nishio J, Iwasaki H, Yoshida K, Gohda J, Inoue J, Watanabe S, Semba K
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Journal Title
Am J Pathol 163(6)
Pages: 2165-2172
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Establishment of a novel human dedifferentiated liposarcoma cell line, FU-DDLS-1 : conventional and molecular cytogenetic characterization2003
Author(s)
Nishio J, Iwasaki H, Ishiguro M, Ohjimi Y, Fujita C, Ikegami H, Ariyoshi A, Naito M, Kaneko Y, Kikuchi M
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Journal Title
Int J Oncol 22(3)
Pages: 535-542
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A tetraspanin-family protein, T-cell acute lymphoblastic leukemia-associated antigen 1, is induced by the Ewing's sarcoma-Wilms' tumor 1 fusion protein of desmoplastic small round-cell tumor.2003
Author(s)
Ito E, Honma R, Imai J, Azuma S, Kanno T, Mori S, Yoshie O, Nishio J, Iwasaki H, Yoshida K, Gohda J, Inoue J, Watanabe S, Semba K
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Journal Title
Am J Pathol 163(6)
Pages: 2165-2172
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Establishment of a novel human dedifferentiated liposarcoma cell line, FU-DDLS-1 : conventional and molecular cytogenetic characterization.2003
Author(s)
Nishio J, Iwasaki H, Ishiguro M, Ohjimi Y, Fujita C, Ikegami H, Ariyoshi A, Naito M, Kaneko Y, Kikuchi M.
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Journal Title
Int J Oncol 22
Pages: 535-42
Description
「研究成果報告書概要(欧文)」より
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