Molecular mechanism for memory B cell dynamics and survival

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 15390164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: NATIONAL INSTITUTE OF INFECTIOUS DISEASES
• Principal Investigator: TAKEMORI Toshitada NATIONAL INSTITUTE OF INFECTIOUS DISEASES, DEPARTMENT OF IMMUNOLOGY, DIRECTOR, 免疫部, 部長 (60114295)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Yoshimasa NATIONAL INSTITUTE OF INFECTIOUS DISEASES, DEPARTMENT OF IMMUNOLOGY, SENIOR RESEARCHER, 免疫部, 主任研究官 (60311403) ; 藤猪 英樹 国立感染症研究所, 免疫部, 研究員 (50356250) ; HASHIMOTO Shuichi NATIONAL INSTITUTE OF INFECTIOUS DISEASES, DEPARTMENT OF IMMUNOLOGY, RESEARCHER, 免疫部, 研究員 (60342896)
• Project Period (FY): 2003 – 2005
• Keywords: memory B cells / Germinal center / somatic mutations / survival / apoptosis / Ras / affinity selection

Research Abstract

Chemotactic chemokines play important role in the selective recruitment of B cells to different histological tissues, establishing B cell homeostasis and immune response. Here we report that CC chemokine receptor D6 and CCR1 is differentially expressed on MZ and memory B cells in the spleen and B1 cells in the peritoneal cavity. In agreement with previous observations, D6 on B cell subsets physically interact with its ligands, internalize, and target them intracellular degradation, whereasa it did not cause activation of MZ B cells and B1 cells, such as proliferation, terminal differentiation. The results suggest that the genuine function of D6 on B cell subsets is to eliminate CC chemokines from microenvironment surrounding MZ B cell and B1 cells. In addition, during the course of search for memory B cells' specific genes, we have identified the gene 3940 who express at high levels in memory cells and marginal zone B cells. Over expression of 3940 in B cell lymphoma cell lines resulted in the prolongation of cell survival under the pro-apoptoic conditions, suggesting that 3940 may play some role in survival of memory B cells.

Research Products

• [Journal Article] A unique role of Ras in memory B cell response. (2005)
  • Author(s): Takahashi, Y., et al.
  • Journal Title: Immunity 23 Pages: 127-138
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Two waves of meniory B cell generation in the primary immune response. (2005)
  • Author(s): Inamine, A., et al.
  • Journal Title: Int. Immnol. 17 Pages: 581-589
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A unique role of Ras in memory B cell response. (2005)
  • Author(s): Takahashi, Y., Inamine A., Hashimoto, S.-I., Yoshioka, E., Kojima, N., Abe, R., Takemroi T.
  • Journal Title: Immunity 23 Pages: 127-138
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Two waves of memory B cell generation in the primary immune response. (2005)
  • Author(s): Inamine, A., Takahashi, Y., Tokuhisa, T., Miyae K., Takemori, T., Abe, R.
  • Journal Title: Int.Immunol. 17 Pages: 581-589
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An essential role for the RNA-pr b GANP for somatic hypermutation of immunoglobulin gene in genninal center B cells. (2004)
  • Author(s): Kuwahara, K. et al.
  • Journal Title: Proc. NatI. Acad. Sci. USA 101 Pages: 1010-1015
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Studies on the generation and maintenance of mucosal Cytotoxic T Lymphocytes against human immunodeficiency virus type-1 Gag in mice. (2003)
  • Author(s): Yoshizawa, I., et al.
  • Journal Title: Aids Res. Hum. Retro. 19 Pages: 469-479
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of recombination-activating gene in mature peripheral T cells in Peyer's patch. (2003)
  • Author(s): Kondo, E., et al.
  • Journal Title: Int. Immunol. 15 Pages: 393-402
  • Description: 「研究成果報告書概要(和文)」より