2006 Fiscal Year Final Research Report Summary
Study on the practical usefulness of myoglobin from the aspects of forensic diagnosis
| Project/Area Number |
15390214
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Okayama University |
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Principal Investigator |
MIYAISHI Satoru Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor, 大学院医歯薬学総合研究科, 助教授 (90239343)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIZU Hideo Kawasaki University of Medical Welfare, Faculty of Health and Welfare, Professor, 医療福祉学部, 教授 (70033157)
YAMAMOTO Yuji Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Lecturer, 大学院医歯薬学総合研究科, 講師 (30136379)
YOSHITOME Kei Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant, 大学院医歯薬学総合研究科, 助手 (40304307)
KITAO Takashi Ehime Prefectural University of Health Sciences, Faculty of Health Sciences, Lecturer, 健康科学部, 講師 (60218047)
TAKATA Tomoyo Ehime Prefectural University of Health Sciences, Faculty of Health Sciences, Lecturer, 健康科学部, 講師 (70310894)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Myogloin / Forensic diagnosis / Postmortem interval |
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| Research Abstract |
Postmortem change of blood myoglobin concentration was experimentally investigated using rabbits. We encountered peculiar specimens two times during the term of the project, which we could not determined the assayed value. Although we could neither find the reason nor establish the concrete criterion for the estimation of time since death from the myoglobin level in postmortem blood, it was concluded that this method could be useful for the determination the time within about 12 hours postmortem. In the experiment of determining postmortem interval by the quantitative analysis of DNA degradation in myoglobin gene using mouse, it became clear that the amount of amplified products had depended on the postmortem interval, the length of the fragment amplified and the temperature in which the dead body had been left. This analysis was thought to be useful for estimating the postmortem interval of some weeks after death. Such principle like this seemed to be applicable to forensic autopsy cases. Postmortem exudation of myoglobin from the striated muscle and its diffusion were also experimentally investigated using mouse. Deletion of myoglobin was observed in the striated muscle by immunostaining as very early stage of postmortem change and this substance became visible again somewhat later. Such exudation of myoglobin could occur during the routine formalin-fixation and it was suggested that the same postmortem change could be observed about some other proteins in striated muscle. This means that the criteria of microscopic diagnosis of some causes of death using formalin-fixed myocardium/skeletal muscle must be revised.
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Research Products
(6 results)
