2004 Fiscal Year Final Research Report Summary
Functional analyses of gastric mucosa using mice deficient in both histamine H_2 receptor and CCK_B/gastrin receptor
| Project/Area Number |
15390227
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ISHIKAWA Takashi The University of Tokyo, Health Service Center, Lecturer, 保健センター, 講師 (50202958)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Yasushi The University of Tokyo, Faculty of Medicine, Medical Stuff, 医学部附属病院, 医員
TATEISHI Keisuke The University of Tokyo, Faculty of Medicine, Medical Stuff, 医学部附属病院, 医員
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| Project Period (FY) |
2003 – 2004
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| Keywords | histamine H2 receptor / gastrin receptor / gastric mucosa / gastric acid secretion |
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| Research Abstract |
To decipher the roles of gastrin and histamine H_2 receptors in gastric mucosa, mice deficient in both were generated and analyzed. Double-null mice exhibited atrophy of gastric mucosae, marked hypergastrinemia and higher gastric pH than gastrin receptor-null mice, which was unresponsive even to carbachol. Comparison of gastric mucosae from 10-week-old wild type, H_2 receptor-null, gastrin receptor-null and double-null mice revealed unique roles of these receptors in gastric mucosa. While small parietal cells and increases in the number and mucin contents of mucous neck cells were secondary to impaired acid production, H_2 receptor was responsible for chief cell maturation. In double-null and gastrin receptor-null mice, despite gastric mucosal atrophy, surface mucous cells were significantly increased, in contrast to gastrin-null mice. Other gastrin-gene product than gastrin-17 may be responsible for the increase independently of the H_2 receptor. Thus, different GPCRs affect differentiation into different cells derived from common stem cells in gastric mucosa.
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Research Products
(8 results)
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[Journal Article] Unique roles of G protein-coupled histamine H2 and gastrin receptors in growth and differentiation of gastric mucosa.2004
Author(s)
Fukushima Y, Matsui T, Saitoh T, Ichinose M, Tateishi K, Shindo T, Fujishiro M, Sakoda H, Shojima N, Kushiyama A, Anai M, Ono H, Oka M, Shimizu S, Nagai R, Kurihara H, Ishikawa T, Asano T, Omata M.
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Journal Title
Eur J Pharmacol 502
Pages: 243-252
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Extremely early onset of ranitidine action on human histamine H2 receptors expressed in HEK293 cells.2003
Author(s)
Fukushima Y, Ishikawa T, Saitoh T, Tateishi K, Ogihara T, Fujishiro M, Shojima N, Honda M, Kushiyama A, Anai M, Sakoda H, Ono H, Onishi Y, Otsuka H, Katagiri H, Nagai R, Omata M, Asano T.
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Journal Title
Digestion 68
Pages: 145-152
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Structural and functional characterization of gastric mucosa and central nervous system in histamine H(2) receptor-null mice.2003
Author(s)
Fukushima Y, Shindo T, Anai M, Saitoh T, Wang Y, Fujishiro M, Ohashi Y, Ogihara T, Inukai K, Ono H, Sakoda H, Kurihara Y, Honda M, Shojima N, Fukushima H, Haraikawa-Onishi Y, Katagiri H, Shimizu Y, Ichinos M, Ishikawa T, Omata M, Nagai R, Kurihara H, Asano T.
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Journal Title
Eur J Pharmacol 468
Pages: 47-58
Description
「研究成果報告書概要(欧文)」より
