2005 Fiscal Year Final Research Report Summary
Development of an effective neural stem cell transplantation method against ischemic brain diseases
| Project/Area Number |
15390434
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
SATO Kohji Hamamatsu University School of Medicine, Professor, 医学部, 教授 (80235340)
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| Co-Investigator(Kenkyū-buntansha) |
OHNO Koji Hamamatsu University School of Medicine, Associated Professor, 医学部, 助教授 (90263277)
MIKAWA Sumiko Hamamatsu University School of Medicine, Assistant, 医学部, 助手 (70359743)
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| Project Period (FY) |
2003 – 2005
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| Keywords | neural stem cell / transplantation / bone morphogenic protein / neuron / astrocyte |
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| Research Abstract |
Neural stem cell transplantation occupies the interest of many researchers who want to cure neuronal and psychological diseases, such as ischemic brain diseases, Parkinson's diseases, and schizophrenia. However, an important issue is that most of transplanted neural stem cells differentiate into glial cells, and only little cells become neuronal cells. One of the reasons is that environmental cues determine the outcome of the differentiation. To overcome this issue, we tried to develop a new neural stem cell transplantation method using a new protein, Kir, which is an antagonist of bone morphogenetic proteins (BMPs). In vitro experiments, we have clearly shown that Kjr protein preferentially differentiates neural stem cells into neural cells. To investingate this effect in iv vivo models, we employed three different ischemic models, i.e. a gerbil 3-min ischemic model, a rat midde cerebral artery occulusion model, and a rat secondary thalami-degeneration model. We transplanted neural stem cells with Kjr protein into these model brains and evaluate the differentiation profiles of these cells using double immunostaing method. We found that Kjr-administered groups showed higher rates of differentiated neuronal cells, suggesting that Kjr is a promising tool for developing an effective neural stem cell transplatation technique.
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Research Products
(12 results)
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[Journal Article] Essential role of endogenous tissue plasminogen activator via matrix metalloproteinase 9 induction and expression on heparin-produced cerebral hemorrhage after cerebral ischemia in mice.2004
Author(s)
Zhao B.Q., Ikeda Y., Ihara H., Urano T., Fan W., Mikawa S., Suzuki Y., Kondo K., Sato K., Nagai N., Umemura K.
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Journal Title
Blood 103
Pages: 2610-2616
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Essential role of endogenous tissue plasminogen activator via matrix metalloproteinase 9 induction and expression on heparin-produced cerebral hemorrhage after cerebral ischemia in mice.2004
Author(s)
Zhao B.Q., Ikeda Y, Ihara H., Urano T., Fan W., Mikawa S., Suzuki Y., Kondo K., Sato K., Nagai N., Umemura K.
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Journal Title
Blood 103
Pages: 2610-2616
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 2. A novel astrocyte-derived secretory factor, neurogenesin-1, provides neurogenic environmental cues for neural stem cells in the adult hippocampus.2003
Author(s)
Ueki T., Tanaka M., Yamashita K., Mikawa S., Qiu Z.F., Maragakis N.J., Hevner R.F., Miura N., Sugimura H., Sato K.
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Journal Title
J. Neurosci. 23
Pages: 11732-11740
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A novel astrocyte-derived secretory factor, neurogenesin-1, provides neurogenic environmental cues for neural stem cells in the adult hippocampus.2003
Author(s)
Ueki T., Tanaka M., Yamashita K., Mikawa S., Qiu Z.F., Maragakis N.J., Hevner R.F., Miura N., Sugimura H., Sato K.
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Journal Title
J.Neurosci. 23
Pages: 11732-11740
Description
「研究成果報告書概要(欧文)」より
