Establishment of immunotherapy for high-grade soft tissue sarcomas using novel antigens.

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 15390462
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: SAPPORO MEDICAL UNIVERSITY
• Principal Investigator: KAWAGUCHI Satoshi SAPPORO MEDICAL UNIVERSITY, MEDICAL SCHOOL, ASSISTANT PROFESSOR, 医学部, 講師 (80315499)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Toshihiko SAPPORO MEDICAL UNIVERSITY, MEDICAL SCHOOL, PROFESSOR, 医学部, 教授 (70244366) ; WADA Takuro SAPPORO MEDICAL UNIVERSITY, MEDICAL SCHOOL, ASSOCIATE PROFESSOR, 医学部, 助教授 (00244369) ; SATO Noriyuki SAPPORO MEDICAL UNIVERSITY, MEDICAL SCHOOL, PROFESSOR, 医学部, 教授 (50158937)
• Project Period (FY): 2003 – 2005
• Keywords: Immunotheraphy / novel antigen / soft tissue sarcome / antigenic peptide / 腫瘍抗原 / CTL / 骨肉腫

Research Abstract

1.Clinical trials of SYT-SSX fusion gene-derived peptide vaccine
We completed the initial immunotherapeutic trial of SYT-SSX-derived antigenic peptide in six patients with disseminated synovial sarcoma, who are positive for HLA-A24. Currently the subsequent vaccination trial is underway using the agretope-modified SYT-SSX peptide.
2.Identification of osteosarcoma novel antigen
We established an osteosarcoma cell line, and cytotoxic T lymphocyte (CTL) clones from the same patient. Using this osteosarcoma-CTL pair, subsequently we cloned an CTL-defined osteosarcoma antigen, Papillomavirus binding factor (PBF). We are currently preparing for a immunotherapeutic trial using PBF-derived peptides as vaccines.
3.Establishment of autologous tumor-CTL pairs
We also established autologous tumor-CTL pairs from patients with malignant fibrous histiocytoma of bone and that of soft tissue, respectively. Using these pairs, we are identifying tumor antigens by cDNA expression cloning

Research Products

• [Journal Article] HLA-restricted specific tumor cytolysis by autologous T lymphocytes infiltrating metastatic bone malignant fibrous histiocytoma of lymph node. (2006)
  • Author(s): Tsukahara T, Kawaguchi S, Ida K, Kimura S, Tamura Y, et al.
  • Journal Title: J Orthop Res 24 Pages: 94-101
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phase I vaccination trial of SYT-SSX junction peptide in patients with disseminated synovial sarcoma. (2005)
  • Author(s): Kawaguchi S, Wada T, Ida K, Sato Y, Nagoya S, Tsukahara T, et al.
  • Journal Title: J Transl Med 3 Pages: 1
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A request for therapeutic antigens in bone and soft tissue sacroma. (2005)
  • Author(s): Kawaguchi S, Wada T, Tsukahara T, Ida K, Torigoe T, Sato N, Ymashita T
  • Journal Title: J Transl Med 3 Pages: 31
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aberrant laminin beta3 isoforms downstream of EWS-ETS fusion genes in Ewing family tumors. (2005)
  • Author(s): Irifune H, Nishimori H, Watanabe G, Yoshida K, Ikeda T, Matsui C, morohashi M, Kawaguchi S, et al.
  • Journal Title: Cancer Cell Ther 4 Pages: 449-455
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evpression of epidermal growth factor receptor,ERBB2 and KIT in adult soft tissue sacromas. (2005)
  • Author(s): Sato O, Wada T, Kawai A, Yamaguchi U, Makimoto A, Kokai Y, et al.
  • Journal Title: Cancer 103 Pages: 1881-1890
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 骨・軟部肉腫に対する腫瘍抗原特異的免疫療法 (2005)
  • Author(s): 川上 哲, 和田卓郎, 名越 智, 塚原智英, 井田和功, 山下敏彦
  • Journal Title: 整災外 48 Pages: 1186-1187
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A quest for therapeutic antigens in bone and soft tissue sarcoma. (2005)
  • Author(s): Kawaguchi S, Wada T, Tsukahara T, Ida K, Torigoe T, Sato N, Yamashita T
  • Journal Title: J Transl Med 3 Pages: 31
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aberrant laminin beta3 isoforms downstream of EWS-ETS fusion genes in Ewing family tumors (2005)
  • Author(s): Irifune H, Nishimori H, Watanabe G, Yoshida K, Ikeda T, Matsui C, Morohashi M, Kawaguchi S, et al.
  • Journal Title: Cancer Cell Ther 4 Pages: 449-455
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of epidermal growth factor receptor, ERBB2 and KIT in adult soft tissue sarcomas. (2005)
  • Author(s): Sato O, Wada T, Kawai A, Yamaguchi U, Makimoto A, Kokai Y, Yamashita T, Chuman H, Beppu Y, Tani Y, Hasagawa T.
  • Journal Title: Cancer 103 Pages: 1881-1890
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] New MOOK 整形外科 (2005)
  • Author(s): 川上 哲, 和田卓郎
  • Total Pages: 260
  • Publisher: 金原出版
  • Description: 「研究成果報告書概要(和文)」より