2005 Fiscal Year Final Research Report Summary
New aspects of the mechanism of emergence from anesthesia -A research for the antagonistic action of orexin, a cerebral excitatory neurotransmitter, against anesthesia-
| Project/Area Number |
15390472
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Fukui (2004-2005)
福井医科大学 (2003) |
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Principal Investigator |
FUKUDA Satoru University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (30116751)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Hisato University of Fukui, Faculty of Medical Sciences, lecturer, 医学部, 助手 (90235987)
YASUDA Yoshikazu University of Fukui, Faculty of Medical Sciences, Assistant Professor, 医学部附属病院, 講師 (50252002)
HIGUCHI Takashi University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (70106326)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Orexin / Hypothalamus / Chorinergic arousal system / Basal forebrain / Isoflurane / Electroencephalogram / Acetylcholine / Glutamate |
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| Research Abstract |
Orexin has been reported to have the various actions of increases in feeding, maintenance of wakefulness, pain relief as well as activation of sympathetic nervous system. Among these actions of orexin, the maintenance of wakefulness induced by orexins might contribute to the emergence from anesthesia. In the brain, there are various arousal systems such as noradrenergic, cholinergic, histaminergic, serotonergic and dopaminergic systems. It has been reported that the cholinergic arousal system may contribute to the emergence from isoflurane, sevoflurane, propofol and neuroleptanestesia. Thus, we focused on the role of cholinergic system on the emergence from anesthesia in relation to orexinergic system. From our studies, we got the following results. 1) Intraventricular injection of orexin activated the electroencephalogram (EEG) under deep isoflurane anesthesia in the rat. 2) Microinjection of glutamate into the posterior hypothalamus induced EEG arousal through acting on the orexin ne
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urons. 3) The selective destruction of lateral hypothalamus with orexin-saporin decreased the minimum alveolar concentration of isoflurane. 4) Isoflurane dose-dependently increased the efflux of glutamate in the basal forebrain, not in the posterior hypothalamus and the cortex. Further, under isoflurane anesthesia, microinjection of AMPA, a glutamate receptor agonist, dose-dependently increased the efflux of acetylcholine (Ach) from the cortex, suggesting that the anesthetic level of isoflurane may be balanced between the inhibitory action of isoflurane and the excitatory action of glutamate induced by this agent. 5) Under isoflurane anesthesia, microinjection of orexins into the basal forebrain induced the increases in Ach efflux and activated EEG. In addition, the electrical stimulation of the pedunculopontine tegmentum, the origin of cholinergic ascending pathways, increased the cortical Ach efflux and the EEG arousal under isoflurane anesthesia. These actions were antagonized by SB334867, a selective orexin-1 receptor antagonist. From all the findings, we concluded that orexinergic system may contribute to the emergence from anesthesia through cholinergic arousal system. Less
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Research Products
(8 results)
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[Journal Article] オレキシン2004
Author(s)
鈴木久人, 安田善一, 江口広毅, 菫 海龍, 立山博一, 伊佐田哲朗, 竹内健二, 溝口恭子, 信川泰成, 冨田優子, 冨士原秀善, 福田 悟
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Journal Title
臨床麻酔 28・8
Pages: 1377-1383
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Orexins2004
Author(s)
Suzuki H, Yasuda Y, Eguchi H, Dong HL, Tateyama H, Isada T, Takeuchi K, Mizoguchi K, Nobukawa Y, Tomita Y, Fujihara H, Fukuda S
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Journal Title
Clinical Anesthesia 28-8
Pages: 1377-1383
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Orexin A elicits arousal electroencephalogram without sympathetic cardiovascular activation in isoflurane-anesthetized rats2003
Author(s)
Yasuda Y, Takeda A, Fukuda S, Suzuki H, Ishimoto M, Mori Y, Eguchi H, Saitoh R, Fujihara H, Honda K, Higuchi T.
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Journal Title
Anesth & Analg 97
Pages: 1663-1666
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Orexin A elicits arousal electroencephalogram without sympathetic cardiovascular activation in isoflurane-anesthetized rats.2003
Author(s)
Yasuda Y, Takeda A, Fukuda S, Suzuki H, Ishimoto M, Mori Y, Eguchi H, Saitoh R, Fujihara H, Honda K, Higuchi T
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Journal Title
Anesth Analg 97
Pages: 1663-1666
Description
「研究成果報告書概要(欧文)」より
