2005 Fiscal Year Final Research Report Summary
Abnormal proliferative mechanisms in prostate : Multiple molecular pathobiologic analyses in microenvironment
| Project/Area Number |
15390489
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Mie University |
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Principal Investigator |
SUGIMURA Yoshiki Mie University, Graduate School of Medicine, Nephro-Urologic Surgery and Andrology, Professor, 大学院医学系研究科, 教授 (90179151)
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| Co-Investigator(Kenkyū-buntansha) |
ARIMA Kiminobu Mie University, Graduate School of Medicine, Nephro-Urologic Surgery and Andrology, Associate Professor, 大学院医学系研究科, 助教授 (10175995)
ONISHI Takehisa Mie University, University Hospital, Nephro-Urologic Surgery and Andrology, Assistant, 医学部附属病院, 助手 (50293783)
SOGA Norihito Mie University, University Hospital, Nephro-Urologic Surgery and Andrology, Assistant, 医学部附属病院, 助手 (60332714)
ISHII Kenichiro Mie University, Graduate School of Medicine, Nephro-Urologic Surgery and Andrology, Assistant, 大学院医学系研究科, 助手 (90397513)
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| Project Period (FY) |
2003 – 2005
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| Keywords | human prostate cancer / androgen-sensitivity / mesenchymal cells / paracrine signals / epithelial-stromal interaction / tumorigenicity |
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| Research Abstract |
Tumor tissues compose of not only tumor cells but also other components such as stromal cells and blood vessels, which construct tumor microenvironment. Fetal urogenital sinus mesenchyme(UGM) has a characteristic which resembles prostatic cancer-associated fibroblast(CAF). In this study, we investigated the effect of fetal rat UGM(rUGM) which secrets a number of factors as paracrine signals between epithelium and stroma on tumorigenicity of human prostate cancer cells in vivo.
Both of androgen-sensitive LNCaP cells and androgen-insensitive LNCaP subline AIDL cells gave rise to the formation of well-defined globular tumors containing large blood-filled areas at both sub-cutaneous and sub-renal capsule grafting sites. There was no significant difference of gross appearance and histo-pathology between LNCaP and AIDL cells.
Although both LNCaP and AIDL xenografts without fetal rUGM contained large blood-filled areas, recombinants with fetal rUGM reduced those blood-filled areas and increased cancer cell growth. Stromal cells derived from fetal rUGM were observed surrounded cancer cells. Tumor vessel structure in stromal components was. seen as a more uniform appearance of stable vessels. Tumor volume as estimated cancer cell growth with fetal rUGM was calculated. The size of AIDL with fetal rUGM was approximately three times compared with that of LNCaP with fetal rUGM. AR and E-cadherin immunostaining in AIDL with fetal rUGM were decreased apparently.
Our data in experimental prostatic tumor microenvironment have showed that not only paracrine signals from mesenchymal cells but also androgen-sensitivity of cancer cells plays an important role in tumor microenvironment.
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Research Products
(12 results)
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[Journal Article] Steroid hormones stimulate human prostate cancer progression and metastasis2006
Author(s)
Ricke, WA., Ishii, K., Ricke, EA., Simko, J., Wang, YZ., et al.
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Journal Title
International Journal of Cancer 118(9)
Pages: 2123-2131
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Natural history of human prostate gland : Morphometric and histopathological analysis of Japanese men2005
Author(s)
Fujikawa, S., Matsuura, H., Kanai, M., Fumino, M., Ishii, K., et al.
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Journal Title
Prostate 65(4)
Pages: 355-364
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Forkhead box A1 regulates prostate ductal morphogenesis and promotes epithelial cell maturation.2005
Author(s)
Gao N, Ishii K, Mirosevich J, Kuwajima S, Oppenheimer SR, Roberts RL, Jiang M, Yu X, Shappell SB, Caprioli RM, Stoffel M, Hayward SW, Matusik RJ.
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Journal Title
Development 132(15)
Pages: 3431-3443
Description
「研究成果報告書概要(欧文)」より
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