2004 Fiscal Year Final Research Report Summary
Formation of Hydroxyapatite-Mediated Three-Dimensional Structures by Mouse Fibroblasts in Response to Physical Stimulation.
| Project/Area Number |
15500396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kibi International University |
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Principal Investigator |
HIRAGAMI Fukumi Kibi International University, Department of Physical Therapy, Professor, 保健科学部, 教授 (60278976)
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| Co-Investigator(Kenkyū-buntansha) |
KANO Yoshio Kibi International University, Department of Occupational Therapy, Professor, 保健科学部, 教授 (70116200)
AKIYAMA Junichi Kibi International University, Department of Physical Therapy, Associate Professor, 保健科学部, 助教授 (00309600)
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| Project Period (FY) |
2003 – 2004
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| Keywords | C3H10T1 / 2 cells / Heat treatment / 3D-like proliferation patterns / Thermotherapy / Hyperthermia |
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| Research Abstract |
The aim of this study was to determine the minimal conditions in which heat treatment is effective and the optimal heat conditions for enhancing three-dimensional (3D)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5 or 43℃ for 2, 10, 15, 20, 45, 60 and 180 min. Then minimal and optimal conditions of temperature and duration of heat treatment for induction of 3D-like proliferation of cells were determined. The minimal conditions of heat treatment to induce 3D-like proliferation were 43℃ for 2 min and the optimal conditions were 43℃ for 10 min. The mean rates of formation of 3D-like proliferation patterns by cells heat-treated at 43℃ for 2 and 10 min were significantly higher (1.7-fold and 3.7-fold higher, respectively) than that by untreated cells (p<0.05). We also observed significantly greater effects of heat treatment on 3D-like proliferation at 40℃ for 180 min and at 41.5℃ for 15 min. We found that apoptosis had occurred in almost 80% of cells at one week after heat treatment at 43℃ for 10 min. These findings suggest that heat shock is associated with 3D-like cell proliferation under optimal conditions. The results of this study should be useful for further studies aimed at elucidation of the physiologic mechanisms underlying thermotherapy and hyperthermia.
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Research Products
(24 results)
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[Journal Article] p38 MAP Kinase Regulating Neuronal Cell Function2005
Author(s)
Kano, Y., Hiragami, F., Kawamura.K., Numata, K., Murai, H., Koike, Y., Shimada, K.
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Journal Title
Journal of KIBI International University School of Health Science 10
Pages: 1-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Low-Frequency Vibratory Sound Induces Neurite Outgrowth in PC12M3 Cells in which Nerve Growth Factor-Induced Neurite Outgrowth is Impaired2004
Author(s)
Koike, Y., Iwamoto, S., Kimata, Y., Nohno, T., Hiragami, F., Kawamura, K., Mumata, K., Mural, H., Okisima, K., Iwata, M., Kano, Y.
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Journal Title
Tissue Culture Research Communication 23
Pages: 81-90
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Heat Shock Induces Neurite Outgrowth in PC12m3 cells via the p38 Mitogen-Activated Protein Kinase Pathway2004
Author(s)
Kano, Y., Nakagiri, S., Nohno, T., Hiragami, F., Kawamura, K., Kadota, M., Numata, K., Koike, Y., Furuta, T.
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Journal Title
Brain research 1026
Pages: 302-306
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Human Placenta Feeder Layers Support Undifferentiated Growth of Primate Embryonic Stem Cells2004
Author(s)
Miyamoto, K., Hayashi, K., Suzuki, T., Ichihara, S., Yamada, T., Kano, Y., Yamabe, T., Ito, Y.
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Journal Title
Stem Cells 22
Pages: 433-440
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Heat Shock-Induced Formation of Three-Dimensional-Like Proliferation Mediated of Hydroxyapatite in Mouse Fibroblasts2004
Author(s)
Hiragami, F., Inoue, S., Akiyama, J., Hidaka, M., Koike, Y., Numata, K., Kano, Y.
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Journal Title
Journal of The Japanese Society of Physio Therapeutics 11
Pages: 13-19
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neurotransmitter Induce Neurite Outgrowth in PC 12 Mutant Cells with Impaired NGF-promoted Neuritogenesis2004
Author(s)
Kano, Y., Yamaguchi, R., Tanabe, K., Numata, K., Okishima, K., Iwata, M., Koike, Y., Shimada, K.
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Journal Title
Journal of KIBI International University School of Health Science 9
Pages: 157-163
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Establishment of Neurotransmitter-Hypersensitive PC12 Variant Cells Deficient in Nerve Growth Factor-Induced Neurite Outgrowth2003
Author(s)
Yamaguchi, R., Hiragami, F., Kawamura, K., Shimada, K., Gomita, Y., Kano, Y
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Journal Title
Tissue Culture Research Communication 22
Pages: 101-108
Description
「研究成果報告書概要(欧文)」より
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