2004 Fiscal Year Final Research Report Summary
A new approach for the prevention of osteoporosis protective roles of plant-derived isoplenoids.
| Project/Area Number |
15500560
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Nakamura Gakuen University |
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Principal Investigator |
TSUDA Hinoko Nakamura Gakuen University, Dept.of Nutritional Sciences, Professor, 栄養科学部, 教授 (30180003)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Keiko Nakamura Gakuen University, Dept.of Nutritional Sciences, Professor, 栄養科学部, 教授 (30069786)
TAKAYANAGI Ryoichi Kyushu University, Graduate School of Medicine, Dept.of Geriatric Medicine, Professor, 大学院・医学研究院・老年医学, 教授 (30154917)
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| Project Period (FY) |
2003 – 2004
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| Keywords | dietary habits / osteoporosis / plant-derived isoplenoid / osteoblast |
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| Research Abstract |
(1)Basic study
Plant-derived monoterpenes, perillyl alcohol, perillic acid and geraniol, were found to enhnance alkaline phosphatase(ALP) activity and inhibit proliferation of the human osteoblast cell line. In addition, the reverse transcription-polymerase chain reaction(RT-PCR) assay revealed that these monoterpenes increased the expression of mRNA for the osteoblat-differentiation markers including ALP in the osteoblast cell line. The role of inhibition of 3-hydroxy-3-methylglutar-yl coenzyme A reductase in promoting bone foemation by these monoterpenes is now under investigation.
To explore whether plant-derived monoterpenes prevent glucocorticoid-induced osteoporosis, one of the most prevalent secondary osteoporosis, we first analysed the molecular mechanism of suppression of bone formation by glucocorticoid. Dexamethasone markedly increased the expression of mRNA for dickkopf-1, an antagonist of Wnt signaling, in primary cultured human osteoblasts. The nuclear translocation of β-ca
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tenin induced by Wnt3a was inhibited by dexamethasone. Thus, it is suggested that glucocorticoid suppresses the canonical Wnt signal, a crucial regulator for bone formation, in cultured human osteoblast.
To investigate the biological effects of plant-derived monoterpenes, we established an animal model of impaired bone formation by feeding the calcium- acid vitamin D-deficient diet to adolescent rats for 40 days. We confirmed both the contents of calcium and phosphorus and the bone mineral density(BMD) were significantly decreased in the femora of the deficient diet-fed rats. The effect of the oral adrministration of plant-derived monoterpenes on the impaired bone formation of the rats is now under investigation.
(2)Clinical study
To determine the lifestyle-related factors associated with bone strength of postmenopausal women, we measured BMD at right radius and urine type I collagen cross-linked N-telopeptide(NTX), a bone resorption marker, and assessed physical status and lifestyle during childhood and present time of pre-and post-menopausal women. The stepwise regression analyses revealed age and timing of menarche as significant predictive factors for decreased BMD and menopause as a significant predictive factor for increased NTX. The habit of skipping breakfast was found to be significant predictive factors for both decreased BMD and increased NTX, resulting in decreased intakes of vegetables and fruits, soybeans, milk products and noodles. Thus, it is suggested that the habitual low vegetables and fruits intake may be a risk factor for impaired bone strength of postmenopausal women. Less
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Research Products
(17 results)
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[Journal Article] The Subcommittee to Study Diagnostic Criteria for Glucocorticoid-Induced Osteoporosis. Guidelines on the management and treatment of glucocorticoid-induced osteoporosis of the Japanese Society for Bone and Mineral Research (2004).2005
Author(s)
Nawata H, Soen S, Takayanagi R, Tanaka I, Takaoka K, Fukunaga M, Matsumoto T, Suzuki Y, Tanaka H, Fujiwara S, Miki T, Sagawa A, Nishizawa Y, Seino Y
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Journal Title
J Bone Miner Metab. 23(2)
Pages: 105-109
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Protein kinase A potentiates adrenal 4 binding protein/steroidogenic factor 1 transactivation by reintegrating the subcellular dynamic interactions of the nuclear receptor with its cofactors, general control nonderepressed-5/transformation/transcription domain-associated protein, and suppressor, dosage-sensitive sex reversal-1 : a laser confocal imaging study in living KGN cells.2004
Author(s)
Fan W, Yanase T, Wu Y, Kawate H, Saitoh M, Oba K, Nomura M, Okabe T, Goto K, Yanagisawa J, Kato S, Takayanagi R, Nawata H.
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Journal Title
Mol Endocrinol. 18(1)
Pages: 127-141
Description
「研究成果報告書概要(欧文)」より
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[Book] 新版・家庭学事典2004
Author(s)
吉岡優子(分担執筆)
Total Pages
453-454
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
