2004 Fiscal Year Final Research Report Summary
Design and preparation of polymer microcarriers for growth of mouse L cell
| Project/Area Number |
15550186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Kumamoto University |
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Principal Investigator |
SAKATA Masayo Kumamoto University, Applied Chemistry, Assistant Professor, 工学部, 助手 (60187391)
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| Project Period (FY) |
2003 – 2004
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| Keywords | cell growth / microcarrier / mouse L cell / polycation / pK_a / amino group / charged amino-group content |
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| Research Abstract |
The electrostatic effect of microcarrier's surface on fibroblastic cell growth was studied. Various aminated cellulose beads were prepared as cationic polymer microcarriers. The microcarriers were prepared by immobilization of 1,3Diamino-2-hydroxypropane, polyallylamine, or poly(ε-lysine), respectively, onto cellulose spherical beads. The amino-group content of the microcarriers was adjusted by changing the immobilization condition(amination time and temperature). The anion-exchange capacities of the microcarriers were estimated not only as total amino- group content but also functional(charged) amino- group content at pH 7.5(cell culture condition). When the growth of mouse L cell on the various microcarriers were determined by common cell culture methods, it strongly depended on the microcarrier's charged amino-group amount at pH 7.5, but not necessarily on their total amino-group content. The charged amino-group content was also drastically controlled by apparent pK_a of the ligand of the microcarriers. As a result, when the cellulose beads with charged amino-group content of 0.02-0.06 meq/g, under cell culture conditions, were used as microcarriers, they showed the excellent growth of mouse L cells.
The present results suggest that the optimum environment for the cell growth is controlled by microcarrier's functional amino-group content but not necessarily by their introduced amino-group content. Their functional amino-group contents were estimated as the charged amino-group content at pH 7.5, which is cell culture pH condition, and it is strongly influenced by their pK_<a,app> values originating from the original amines.
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