2004 Fiscal Year Final Research Report Summary
A study on novel regulation mechanism to repress precocious meiotic entry in Drosophila male
| Project/Area Number |
15570006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
INOUE Yoshihiro Kyoto Institute of Technology, Drosophila Genetic Res.Center, Lecturer, ショウジョウバエ遺伝資源センター, 講師 (90201938)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Masamitsu Kyoto Institute of Technology, Dept.of Applied Biology, Professor, 繊維学部, 教授 (00182460)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Drosophila / male / meiosis / Mitosis / Cyclin / Polo |
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| Research Abstract |
The G2 phase just prior to first meiotic division in male is called a growth phase that is an essential period to ensure gene expression required for meiotic divisions and later spermiogenesis in Drosophila. A timing to initiate the first meiotic division is therefore strictly regulated by activity of CDK1. A hypomorphic mutation at the mitotic gene, meteor (mtr) in Drosophila leads to a production of smaller cells in premeiotic cysts consisting of primary spermatocytes. We found that a chromosome condensation has precociously proceeded in the "wee" cells. Another distinctive features in M phase such as nuclear membrane-break-down and formation of spindle-like structure have also already occurred in these "wee" cells within premeiotic cysts. The precocious entry of meiotic divisions did not allow these cells to grow sufficiently. These cytological observations suggest that the mtr gene is involved in a negative regulation to repress initiation of first meiotic division until a completi
… More
on of the growth phase in Drosophila males. Surprisingly, these precocious meiotic entry in the mtr males occurred before a sufficient expression of both Cyclin A and Cyclin B. Immunostaining experiments to detect active form of CDK1 indicate that the active MPF has not yet accumulated in cytoplasm of the "wee" cells with precociously condensed chromatin in the mtr males. Consistently, this precocious meiotic entry in mtr was observed even in mutant background of twine gene encoding a Cdc25 orthologue exclusively active before meiotic division. In addition to the precocious meiotic entry, a failure of centrosome separation, spindle abnormality and non-disjunction was also observed during meiotic divisions in the mtr males. The mtr gene corresponds to dlarp that encode a Drosophila ortholog of the LA-related protein sharing a homologous domain with human Lupas Antigen. Our immunostaining experiments localized the Meteor protein to a peripheral region of nucleus at premeiotic stages and to the centrosomes at M phase. We speculate that the Meteor has essential roles in both meiotic entry and meiotic divisions in Drosophila males. Less
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Research Products
(21 results)
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[Journal Article] Redox regulation of DNA binding activity of DREF in Drosophila.2004
Author(s)
Choi, T.-Y., Park, S.Y., Kang, H.-S., Cheong, J.-H., Kim, H.-D., Lee, B.L., Hirose, F., Yamaguchi, M., Yoo, M.-A.
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Journal Title
Biochem.J. 378
Pages: 833-838
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transcriptional regulation of the Drosophila caudal homeobox gene by the DRE/DREF system.2004
Author(s)
Choi, Y.-J., Choi, T.-Y., Yamaguchi, M., Matsukage, A., Kim.Y-S., Yoo, M.-A.
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Journal Title
Nucleic Acids Res. 32
Pages: 3734-3742
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] DREF is required for EGFR signaling during Drosophila wing vein development.2004
Author(s)
Yoshida, H., Kwon, E., Hirose, F., Otsuki, K., Yamada, M., Yamaguchi, M.
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Journal Title
Genes to Cells 9
Pages: 935-944
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Armadillo/Pangolin regulates PCNA and DREF promoter activities.2004
Author(s)
Kwon E., Hayashi, Y.Otsuki, K., Hirose, F., Nishida, Y., Yoo, M.-A., Yamaguchi, M.
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Journal Title
Biochem.Biophys.Acta. 1679
Pages: 256-262
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Damaged DNA binding protein 1 in Drosophila defense reactions.2004
Author(s)
Takata, K., Shimanouchi, K., Yamaguchi, M., Murakami, S., Ishikawa, G, Takeuchi, R., Kanai, Y., Ruike, T., Nakamura, R., Abe, Y., Sakaguchi, K.
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Journal Title
Biochem.Biophys.Res.Commun. 323
Pages: 1024-1031
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of dRFX2, a novel RFX family protein in Drosophila.2004
Author(s)
Otsuki, K., Hayashi, Y., Kato, M., Yoshida, H., Yamaguchi, M.
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Journal Title
Nucleic Acids Res. 32
Pages: 5636-5648
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development.2003
Author(s)
Takata, K, Inoue, Y.H., Hirose, F., Murakami, S., Shimanouchi, S., Sakimoto, I., Sakaguchi, K.
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Journal Title
Cell Biology International 27
Pages: 361-374
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions anddevelopment of the polarized microtubule network that interconnects oocyte and nurse cells during oogenesis.2003
Author(s)
Mathe, E., Inoue, Y.H., Palframan, W., Brown, G., Glover, D.M.
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Journal Title
Development 130
Pages: 901-915
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transcription control of a gene for Drosophila transcription factor, DREF by DRE and cis-elements conserved between Drosophila melanogaster and virilis.2003
Author(s)
Kwon, E., Seto, H., Hirose, F., Ohshima, N., Takahashi, Y., Nishida, Y., Yamaguchi, M.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prevention of polyglutamine oligomerization and neurodegeneration by the peptide inhibitor QBP1 in Drosophila.2003
Author(s)
Nagai, Y., Fujikake, N., Ohno, K., Higashiyama, H., Popiel, H.A., Rahadian, J., Yamaguchi, M., Strittmatter, W.J., Burke, J.R., Toda, T.
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Journal Title
Hum.Mol.Genet. 12
Pages: 1253-1260
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Drosophila damaged DNA binding protein 1(D-DDB1) is an essential factor for development.
Author(s)
Takata, K., Yoshida, H., Yamaguchi, M., Shimanouchi, K., Murakami, S., Sakaguchi, K.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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