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2004 Fiscal Year Final Research Report Summary

How differential distributions of actin-bindingproteins are formed in living cells.

Research Project

Project/Area Number 15570153
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionGunma University

Principal Investigator

ISHIKAWA Ryoki  Gunma University, Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (20212863)

Co-Investigator(Kenkyū-buntansha) KOHAMA Kazuhiro  Gunma University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (30101116)
KUMAGAI Hiroyuki  Gunma University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20321945)
Project Period (FY) 2003 – 2004
Keywordsactin / tropomyosin / actin-binding protein / fascin / drebrin
Research Abstract

We examined the distribution and dynamics of actin-binding proteins in Ng108-15 cells. EGFP/fascin localized in filopodia. Pseudo-unphosphorylated mutant, in which 39-serine of fascin was replaced to asparatic acid, localized in filopodia while pseudo-phosphorylated mutant, in which 39-serine was replaced to alanine, showed dispersed distribution, suggesting that localization of fascin is regulated by phosphorylation. EGFP/smooth muscle type tropomyosin localized only in stress fibers, while EGFP/nonmusle type tropomyosin localized in stress fibers, filopodia, and lamellipodia. EGFP/caldesmon localized in all the actin structures.
Reconstituted characterization of purified proteins revealed that fascin formed tight F-actin bundles with polarity. Drebrin bound to F-actin with a stoichiometry of 5 actin versus 1 drebrin molecule. Drebrin inhibited the actin-binding of caldesmon, actin-binding of tropomyosin, and actin-binding of fascin.
These results suggest that competitive binding between drebrin and tropomyosin/caldesmon, between drebrin and fascin, and between fascin and tropomyosin/caldesmon may cause the differential localization fascin, drebrin, and tropomyosin/caldesmon, resulting in different organization of actin structure.

  • Research Products

    (10 results)

All 2004 2003 Other

All Journal Article (9 results) Book (1 results)

  • [Journal Article] Calmodulin-dependent cyclic nucleotide phosphodiesterase(PDE1) is a pharmacological target of DIF-1,an anti-tumor agent isolated from Dictyostelium2004

    • Author(s)
      Suzuki K et al.
    • Journal Title

      Cancer Res. 64

      Pages: 2568-2571

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Calmodulin-dependent cyclic nucleotide phosphodiesterase(PDE1) is a pharmacological target of DIF-1, an anti-tumor agent isolated from Dictyostelium.2004

    • Author(s)
      Suzuki K. et al.
    • Journal Title

      Cancer Res. 64

      Pages: 2568-2571

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Polarized actin bundles formed by human fascin-1:Their sliding and disassembly on myosin II and myosin V in vitro.2003

    • Author(s)
      Ishikawa R.et al.
    • Journal Title

      J.Neurochem. 87

      Pages: 676-685

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Zinc inhibits calcineurin activity in vitro by competing with nickel.2003

    • Author(s)
      Takahashi I.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 307

      Pages: 64-68

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Actin dynamics in neuronal growth cone revealed with a polarized light microscopy.2003

    • Author(s)
      Katoh K, Yoshida F, Ishikawa, R
    • Journal Title

      Adv.Exp.Med.Biol. 538

      Pages: 347-358

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Polarized actin bundles formed by human fascin-1 : Their sliding and disassembly on myosin II and myosin V in vitro.2003

    • Author(s)
      Ishikawa, R., et al.
    • Journal Title

      J.Neurochem. 87

      Pages: 678-685

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Zinc inhibits calcineurin activity in vitro by competing with nickel.2003

    • Author(s)
      Takahashi I et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 307

      Pages: 64-68

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Actin dynamics in neuronal growth cone revealed with a polalized light microscopy.2003

    • Author(s)
      Katoh, K., Yoshida, F., Ishikawa, R.
    • Journal Title

      Adv.Exp.Med.Biol. 538

      Pages: 347-358

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Actin, actin-binding proteins and myosin in nervous system.

    • Author(s)
      Ishikawa, R.
    • Journal Title

      Handbook of Neurochemistry and Molecular Neuroviology Vol.7(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Actin, actin-binding proteins and myosin in nervous system. in Handbook of Neurochemistry and Molecular Neuroviology. Vol.7

    • Author(s)
      Ishikawa, R.
    • Publisher
      Springer, New York(印刷中)
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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