2004 Fiscal Year Final Research Report Summary
Cl^-homeostasis failure and abnormal cell migration by endocrine disruptor cause damage to nervous system
| Project/Area Number |
15590207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Shizuoka University |
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Principal Investigator |
YAMADA Junko Shizuoka University, Graduate School of Electronic Science and Technology, Research Associate, 大学院・電子科学研究科, 助手 (30334965)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Atsuo Hamamatsu University School of Medicine, Department of physiology, Professor, 医学部, 教授 (50254272)
INOUE Koichi Hamamatsu University School of Medicine, Department of physiology, Research Associate, 医学部, 助手 (80345818)
OKABE Akihito Hamamatsu University School of Medicine, Department of physiology, Research Associate, 医学部, 助手 (10313941)
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| Project Period (FY) |
2003 – 2004
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| Keywords | development / Chrolide / Cl^-homeostasis / GABA receptor / Tributyotin / GFP / in utero electropolation / neocortex |
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| Research Abstract |
Measurement of [Cl^-]i and cell miglation
Using in vivo electropolation, we transfected eGFP prasmid embryonic day 14 (E14) mice. After electropolation, mice were exposed tributyltin chloride (TBTCl ; 1μl) via amniotic fluid. 3-4 days after exposure TBTCl, mice were observed their conditions.
Results were as follows ;
1.TBTCl 1M : Mice were dead with atrophy. Both side of mice, which were intact, also dead.
2.500 mM-100 mM : Mice were dead with atrophy. Both side of mice, which were intact, survived.
3.10 mM : Mice were dead but looks were normal.
4.1 mM : Mice sere survived and cell miglation was normal.
The effect of TBTCl exposure on Cl^-homeostasis.
Using gramicidin perforate patch-clamp method, we measured [Cl^-]_i TBTCl exposed cell (30 nM, 3days) and intact cell (0.1 % DMSO, 3 days). Both cell group had normal [Cl^-]_i.
The effect of TBTCl exposure on GABA receptor expression
mRNAs of TBTCl exposed cell and control cell were harvested. Using RT-PCR method, expression of GABAA receptor subunit (α1-5、β1-3、γ1-3、δ、ε、and β-actin)were analysed.
The acute effect of TBTCl on culture cell.
Using patch-clamp method and Ca2+ imaging, neurons were observed effect of TBTCl application. At low concentration of TBTCl application (30 nM), there were no effect on membrane potantial and synaptic current. It seems that toxicity at low concentration of TBTCl acts slowly.
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Research Products
(22 results)
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[Journal Article] Differential functional expression of cation-Cl-cotransporter mRNAs (KCC1, KCC2, and NKCC1) in rat trigeminal nervous system.2005
Author(s)
Toyoda, H., Yamada, J., Ueno, S., Okabe A., Kato, H., Sato K., Hashimoto, K, Fukuda, A.
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Journal Title
Molecular Brain Res.5 133(1)
Pages: 12-18
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transgenic Expression Enhanced Green Fluorescent Protein Enables Direct Visualization for Physiological Studies of Vasopressin Neurons and Isolated Nerve Terminals of the Rat.2005
Author(s)
Ueta, Y., Fuj ihara, H., Serino, R., Dayanithi, G., Ozawa, H., Matsuda, K., Kawata, M., Yamada, J., Ueno, S., Fukuda, A., Murphy, D.
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Journal Title
Endocrinology. 146(1)
Pages: 406-413
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Malfunction of respiratory related neuronal activity in Na^+, K^+-ATPase α2 subunit-deficient mice is due to abnormal Cl^-homeostasis in brainstem neurons.2004
Author(s)
Ikeda, K., Onimaru, H., Yamada, J., Inoue, K., Ueno, S., Onaka, T., Toyoda, H., Arata, A., Ishikawa, T., Taketo, M., Fukuda, A., Kawakami, K.
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Journal Title
J.Neurosci. 24
Pages: 10693-10701
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cl^-uptake promoting depolarizing GABA actions in immature rat neocortical neurones is mediated by NKCC1.2004
Author(s)
Yamada, J., Okabe., A., Toyoda, H., Kilb, W., Luhmann, H., Fukuda, A.
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Journal Title
J.Pysiol. 557
Pages: 829-841
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Homogenous glycine receptor expression in cortical plate neurons and Cajal-Retzius cells of neonatal rat cerebral cortex.2004
Author(s)
Okabe, A., Kilb, W., Shimizu-Okabe, C., Hanganu, I.L., Fukuda, A., Luhmann, H.J.
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Journal Title
Neuroscience 123
Pages: 715-724
Description
「研究成果報告書概要(欧文)」より
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