2004 Fiscal Year Final Research Report Summary
Analysis of the neuroendocrine system in PAC1 knock out mice
| Project/Area Number |
15590216
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
SHIBUYA Izumi University of Occupational and Environmental Health, School of Medicine, Associate Professor, 医学部, 助教授 (50162649)
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| Co-Investigator(Kenkyū-buntansha) |
UEZONO Yasuhito Nagasaki University, School of Medicine, Assotiate Professor, 大学院・医歯薬学総合研究科, 助教授 (20213340)
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| Project Period (FY) |
2003 – 2004
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| Keywords | PACAP / PACAP typeI receptor / receptor knock out mouse / supraoptic nucleus / Ca^<2+> imaging / somatodendritic release / vasopressin / oxytocin |
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| Research Abstract |
The role of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor-deficient male mice (PAC1-/-). The effects of PACAP on [Ca2+]i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild-type mice (PAC1+/+), 100 nm PACAP induced an increase in [Ca2+]i and release of vasopressin and oxytocin, whereas in heterozygous (PAC1+/-) and null-mutant mice (PAC1-/-), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC l+/+ and PAC1-/- mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP-induced [Ca2+]i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones.
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Research Products
(14 results)
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[Journal Article] Mechanisms of cytosolic Ca^<2+> suppression by prostaglandin E_2 receptors in rat melanotrophs.2003
Author(s)
Nagata, T., Harayama, N., Sasaki, N., Inoue, M., Toyohira, Y, Uezono, Y., Maruyama, T., Yanagihara, N., Ueta, Y., Shibuya, I.
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Journal Title
Journal of Neuroendocrinology 15
Pages: 33-41
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The inhibitory effects of alphaxalone on M1 and M3 muscarinic receptors expressed in Xenopus oocytes.2003
Author(s)
Shiraishi, M., Minami, K., Shibuya, I., Uezono, Y, Ogata, J., Okamoto, T., Murasaki, O., Kaibara, M., Ueta, Y, Shigematsu, A.
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Journal Title
Anesthesia and Analgesia 97
Pages: 449-455
Description
「研究成果報告書概要(欧文)」より
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