2005 Fiscal Year Final Research Report Summary
Studies on change in airway hyperresponsiveness at bronchial asthma using the technique of RhoA gene manipulation
| Project/Area Number |
15590236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Hoshi University |
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Principal Investigator |
MISAWA Miwa Hoshi University, Department of Pharmacology, School of Pharmacy, 薬理学教室, 教授 (20061294)
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| Co-Investigator(Kenkyū-buntansha) |
CHIBA Yoshihiko Hoshi University, Department of Pharmacology, School of Pharmacy, 薬理学教室, 講師 (00287848)
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| Project Period (FY) |
2003 – 2005
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| Keywords | bronchial asthma / airway hyperresponsiveness / Ca^<2+> sensitization / RhoA / CPI-17 / rhoA mRNA / cpi-17 mRNA / membrane tranlocation |
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| Research Abstract |
(1)The authors have found for the first time that expression of rhoA mRNA and upregulation of RhoA protein in bronchial smooth muscle of airway hyperresponsive (AHR) rats which received repeated antigen-challenge. We propose that enhanced Ca^<2+> sensitization induced by such RhoA protein activation may evoke the AHR. (2)Transcriptional factors involved in RhoA protein expression were investigated by protein/DNA array. Stat5,CDP,HSE,SBE,SIE and CBF were observed to be activated at AHR state. (3)TNF-a detected in broncho-alveolar fluid of asthmatics enhanced the bronchosonstriction by ACh, which accompanied with increases in rhoA mRNA and protein. (4)Translocation of RhoA protein from cytosol to plasma membrane was observed after ACh stimulation of bronchial cells, suggesting an activation of RhoA by ACh. (5)Another protein CPI-17 involved in Ca^<2+> sensitization as well as RhoA was also increased in the AHR bronchial smooth muscle. Therefore, RhoA/Rho-kinase/CPI-17 cross-pathway might work at Ca^<2+> sensitization of bronchial smooth muscle of AHR rats.
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[Journal Article] Uteroglobin-related protein 1 expression suppesses allergic airway inflammation in mice2006
Author(s)
Chiba, Y., Kurotani.R., Kusakabe, T., Miura, T., Link, B.W., Misawa, M., Kimura, S.
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Journal Title
Am. J. Respir. Crit. Care Med. 173
Pages: 958-964
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Uteroglobin-related protein 1 expression suppesses allergic airway inflammation in mice2006
Author(s)
Chiba, Y., Kurotani, R., Kusakabe, T., Miura, T., Link, B.W., Misawa, M., Kimura, S.
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Journal Title
Am.J.Respir.Crit.Care Med. 173
Pages: 958-964
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Involvement of RhoA-mediated Ca^<2+> sensitization in antigen-induced bronchial smooth muscle hyperresponsiveness in mice2005
Author(s)
Chiba, Y., Ueno, A., Shinozaki, K., Takeyama, H., Sakai, H., Misawa, M.
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Journal Title
Respir. Res. 6(#4)
Pages: 1-11
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Effect of cigarette smoke exposure in vivo on bronchial smooth muscle contractility in vitro in rats2005
Author(s)
Chiba, Y., Murata, M., Ushikubo, H., Saitoh, A., Sakai, H., Kamei, J., Misawa, M.
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Journal Title
Am. J. Respir. Cell. Mol. Biol. 33
Pages: 574-581
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Difference in the effects of cigarette smoke exposure on bronchial smooth muscle contractility in rats2005
Author(s)
Chiba, Y., Murata, M., Yoshikawa, Y., Saitoh, A., Sakai, H., Kamei, J., Misawa, M.
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Journal Title
Med. Hypothesis Res. 2
Pages: 597-603
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Involvement of RhoA-mediated Ca^<2+> sensitization in antigen-induced bronchial smooth muscle hyperresponsiveness in mice2005
Author(s)
Chiba, Y., Ueno, A., Shinozaki, K., Takeyama, H., Sakai, H., Misawa, M.
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Journal Title
Respir.Res. 6(#4)
Pages: 1-11
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of cigarette smoke exposure in vivo on bronchial smooth muscle contractility in vitro in rats2005
Author(s)
Chiba, Y., Murata, M., Ushikubo, H., Saitoh, A., Sakai, H., Kamei, J., Misawa, M.
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Journal Title
Am.J.Respir.Cell.Mol.Biol. 33
Pages: 574-581
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Difference in the effects of cigarette smoke exposure on bronchial smooth muscle contractility in rats2005
Author(s)
Chbia, Y., Murata, M., Yoshikawa, Y., Saitoh, A., Sakai, H., Kamei, J., Misawa, M.
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Journal Title
Med.Hypothesis Res. 2
Pages: 597-603
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Acetylcholine-induced translocation of RhoA in freshly isolated single smooth muscle cells of rat bronchi2004
Author(s)
Chiba, Y., Uchida, T., Sakai, H., Oku, T., Ito, S., Tsuji, T., Misawa, M.
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Journal Title
J. Pharmacol. Sci. 95
Pages: 479-482
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Acetylcholine-induced translocation of RhoA in freshly isolated single smooth muscle cells of rat bronchi2004
Author(s)
Chiba, Y., Uchida, T., Sakai, H., Oku, T., Ito, S., Tsuji, T., Misawa, M.
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Journal Title
J.Pharmacol.Sci. 95
Pages: 479-482
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Upregulation of rhoA mRNA in bronchial smooth muscle of antigen-induced airway hyperresponsive rats2003
Author(s)
Chiba, Y., Sakai, H., Wachi, H., Sugitani, H., Seyama, Y., Misawa, M.
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Journal Title
J. Smooth Muscle Res. 39
Pages: 221-228
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Upregulation of rhoA mRNA in bronchial smooth muscle of antigen-induced airway hyperresponsive rats2003
Author(s)
Chiba, Y., Sakai, H., Wachi, H., Sugitani, H., Sayama, Y., Misawa, M.
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Journal Title
J.Smooth Muscle Res. 39
Pages: 221-228
Description
「研究成果報告書概要(欧文)」より
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