2004 Fiscal Year Final Research Report Summary
Proteomic Approach for Protein Expression Profiling of Human Promyelocytic Cells in Response to Infection with Granulocyte- and Monocyte-Tropistic Obligatory Intracellular Microorganisms
| Project/Area Number |
15590394
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
OHASHI Norio University of Shizuoka, Institute for Environmental Sciences, 環境科学研究所, 助教授 (10169039)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAITOU Hirotaka University of Shizuoka, Institute for Environmental Sciences, 環境科学研究所, 助手 (30254262)
IBUKI Yuko University of Shizuoka, Institute for Environmental Sciences, 環境科学研究所, 助手 (30236781)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Keywords | Emerging infectious diseases / Ehrlichiosis / Ehrlichia chaffeensis / Anaplasma phagocytophilum / Proteomics / Host cell response / Obligatory intracellular bacteria / Tick-borne febrile diseases' |
|---|
| Research Abstract |
Anaplama phagocytophilum and Ehrlichia chaffeensis are obligatory intracellular bacteria, agents of emerging infectious diseases (human granulocytic anaplasmosis and human monocytic ehrlichiosis), which infect professional phagocytes with tropism for granulocytes and monocytes/macrophages, respectively, and both are maintained with human promyelocytic cell lines HL-60 in many laboratories. Proteomic approach is a powerful technique to analyze a global protein expression profile in several cellular events of mammalian cell lines and tissues, plants, or bacteria. In this study, we applied to characterize the protein expression profile in host cell response to infection with A. phgocytophiluma and E. chaffeensis by using the most recently introduced DIGE (fluorescence two-dimensional difference gel electrophoresis) system (GE Healthcare, Piscatawy, NJ) which allowed us to detect differences in protein expression between infected and uninfected cells in a single gel. Proteomic changes were monitored in a time course on day 1, 2, and 3 postinfection and this approach revealed a total of 286 protein spots with abundance changes between infected and uninfected cells on day 3 postinfection. Using MALDI TOF/TOF tandem mass spectrometry, we successfully identified the protein species of 154 spots derived from HL-60 cells in response to infections with A. phagocytophilum and E. chafeensis. These protein species are associated with cytoskeleton, signal transduction, translation, stress response and more. MetaCore Pathway analysis (GeneGo Inc., St. Joseph, MI) suggested the candidate proteins of host cells associated with A. phagocytophilum and E. chaffeensis infections, probably these bacteria may reorganize host cell cytoskeleton through the protein-protein interaction to acquire their niches for intracellular survival.
|
-
-
[Journal Article] Seroepidemiology of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in wild mice captured in northern Turkey2005
Author(s)
Guner, ES., Watanabe, M., Kadosaka, T., Polat, E., Gargili, A., Gulanber, A., Ohashi, N., Kaneda, K., Imai, Y., Masuzawa, T.
-
Journal Title
Epidemiol. Infect. 133
Pages: 331-336
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Characterization of Ehrlichia species from Ixodes ovatus ticks at the foot of Mt. Fuji, Japan2004
Author(s)
Inayoshi, M., Naitou, H., Kawamori, F., Masuzawa, T., Ohashi, N.
-
Journal Title
Microbiol. Immunol. 48
Pages: 737-745
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Analysis of p51, groESL, and the major antigen P51 in various species of Neorickettsia, an obligatory intracellular bacterium that infects trematodes and mammals2004
Author(s)
Rikihisa, Y., Zhang, C., Kanter, M., Cheng, Z., Ohashi, N., Fukuda, T.
-
Journal Title
J Clin Microbrol 42
Pages: 3823-3826
Description
「研究成果報告書概要(欧文)」より
-
-
-
