2004 Fiscal Year Final Research Report Summary
Basic study of gene therapy to hepatitis C virus infection by using virus-like particle.
| Project/Area Number |
15590415
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
YASUTOMI Yasuhiro Mie University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90281724)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Keywords | HCV / HEV / immuno gene therapy / VLP |
|---|
| Research Abstract |
Since the infection of virus is likely to occur in populations most often through exposure to mucosal tissue, virus-specific immune responses at mucosal sites are critical for the initial control of infection. A non-replicating vaccine vector to digestive tract mucosa by oral administration is potentially powerful in mucosal vaccine, but suitable vectors have not yet been reported. In the present study, we show two types of novel mucosal vaccine and combined these two attempts by using virus-like particle(VLP) composed of open reading frame 2 of hepatitis E virus(HEV). One is chimeric HEV-VLP having human immunodeficiency virus(HIV) env epitope to stimulate mucosal immunity without the need for adjuvant by oral administration. Another-vaccine using HEV-VLP is attempt to package HIV env DNA in vitro and then to deliver this foreign DNA to intestinal mucosa in vivo as a DNA vaccine carrier. Both these two types of vaccines elicited HIV env specific mucosal and systemic humoral as well as cellular immune responses without any kind of adjuvant through oral administration. These findings provide evidences for the possibility of VLP derived from orally transmissible virus as a vaccine vector to mucosal tissue by oral administration.
|
-
[Journal Article] DNA vaccine-encapsulated virus-like particles derived from an orally transmissible virus stimulates mucosal and systemic immune responses by oral administration.2004
Author(s)
Takamura, S., Niikura, M., Li, T.C., Takeda, N., Kusagawa, S., Takebe, Y., Miyamura, T., Yasutomi, Y.
-
Journal Title
Gene Ther. 11
Pages: 628-635
Description
「研究成果報告書概要(和文)」より
-
-
[Journal Article] Immunization with recombinant bacillus Calmette-Guerin (BCG)-hepatitis C virus (HCV) elicits HCV-specific cytotoxic T lymphocytes in mice.2003
Author(s)
1.Uno-Furuta, S., Matsuo, K., Kim, G., Tamaki, S., Takamura, S., Kamei, A., Kuromatsu, I., Kaito, M., Matsuura, Y., Miyamura, T., Adachi, Y., Yasutomi, Y.
-
Journal Title
Vaccine 20
Pages: 3149-3156
Description
「研究成果報告書概要(和文)」より
-
-
[Journal Article] A single administration of interleukin-4 antagonistic mutant DNA inhibits allergic airway inflammation in a mouse model of asthma.2003
Author(s)
Nishikubo, K., Murata, Y., Tamaki, S., Sugama, K., Imanaka-Yoshida, K., Yuda, N., Kai, M., Takamura, S., Sebald, W., Adachi, Y., Yasutomi, Y.
-
Journal Title
Gene Ther. 10
Pages: 2119-2125
Description
「研究成果報告書概要(和文)」より
-
-
[Journal Article] Immunization with recombinant bacillus Calmette-Guerin(BCG)-hepatitis C virus(HCV) elicits HCV-specific cytotoxic T lymphocytes in mice.2003
Author(s)
Uno-Furuta, S., Matsuo, K., Kim, G., Tamaki, S., Takamura, S., Kamei, A., Kuromatsu, I., Kaito.M., Matsuura.Y., Miyamura, T., Adachi, Y., Yasutomi, Y.
-
Journal Title
Vaccine 21
Pages: 3149-3156
Description
「研究成果報告書概要(欧文)」より
-
