• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2004 Fiscal Year Final Research Report Summary

Brown adipose tissue(BAT) uncoupling protein(UCP) and alcohol in the animal models for alcohol drinking behavior

Research Project

Project/Area Number 15590584
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionKyoto Prefectural University of Medicine Graduated School of Medicine

Principal Investigator

YOSHIMOTO Kanji  Kyoto Prefectural University, Legal Medicine, Associate Professor, 医学研究科, 助教授 (70111903)

Co-Investigator(Kenkyū-buntansha) YSSUHARA Masahiro  Kyoto Prefectural University, Legal Medicine, Professor, 医学研究科, 教授 (60305604)
YANO Tadashi  Meiji University of Oriental Medicine, Acupuncture, Professor, 鍼灸学部, 教授 (70166560)
FUKUDA Fumihiko  Meiji University of Oriental Medicine, Acupuncture, Lecture, 鍼灸学部, 講師 (80238485)
Project Period (FY) 2003 – 2004
Keywordsuncoupling protein / ethanol / RNA / 5-HT2c / receptor / brown adipose tissue / 受容体 / マウス
Research Abstract

Effects of Uncoupling protein-1(UCP1) expression is positively correlated with metabolic inefficiency, being increased by cold acclimation and overfeeding, and reduced in fasting and genetic obesity. It is known that the mitochondrial UCP1 in the brown adipose tissue(BAT) is an important key molecule for non-shivering thermogenesis. On the other hand, ethanol (EtOH) alters thermoregulation in humans and laboratory animals. However, the relationship between EtOH and the UCP1 is not yet clear. Accordingly, the present study employed the technique of real-time quantitative PCR to investigate the effects of EtOH (0,5 and 2.0 g/kg) on the expression of UCP1 mina in the mouse BAT. Control mice were injected with the same volume of physiological saline intraperitoneally(IP). IP injection of 0.5 g/kg EtOH caused a decrease and an increase of the expression of BAT UCP1 mRNA at 1 and 4 hrs, respectively. EtOH treatment at 2.0 g/kg caused an increases of the expression of BAT UCP1 mRNA at both 2 … More and 4 hrs. BAT UCP1 mRNA levels in both groups increased at 4 hrs after EtOH administration. The levels of UCP1 mRNA returned to the control levels by 8 hrs after EtOH administration. The expressions of BAT UCP1 mRNA were upregulated following EtOH administration, although lower doses of EtOH initially suppressed induction of BAT UCP1 mRNA. These findings suggest that EtOH-induced BAT UCP1 mRNA expression corresponded to an alteration of the set point of thermogenesis or feeding behavior.
Finally we examined the effects of EtOH on the induction of UCP1 mRNA in the BAT of inbred strains of mice, C57BL/6J, C3H/HeJ and DBA/2J, with genetic differences of alcohol preference. These inbred strains of mice were injected with EtOH (2.0 g/kg i.p.). Then, one and 8 hrs later, brown adipose tissues(BATs) were collected rapidly and the UCP1 mRNA was extracted. The levels of UCP1 mRNA and β-actin mRNA were measured with the real-time quantitative RT-PCR. Control groups (0 hr) were injected with the same volume of physiological saline. Compared with the two inbred strains of mice (C3H/HeJ and DBA/2J), the basal level of UCP1 mRNA was approximately 70-80% decreased (P<0.01) in the BAT of C57BL/6J. In C3H/HeJ mice, EtOH caused the largest decrease in the level of UCP1 mRNA at one hr after the administration. The level of UCP1 mRNA was decreased significantly 8 hrs later in the DBA/2J mice. On the other hand, EtOH did not alter the induction of UCP1 mRNA in the BAT of C57BL/6J mice. Our findings suggested that EtOH at low to moderate doses induces significant, strain-dependent UCP1 mRNA induction.
The possibility is considered that changes of the EtOH-induced BAT UCP1 mRNA correspond with the genetic differences of the sensitivity to EtOH in the inbred strains of mice. Less

  • Research Products

    (10 results)

All 2004 2003

All Journal Article (10 results)

  • [Journal Article] Alterations in the function of cerebral dopaminergic and serotonergic systems following electroacupuncture and moxibustion.2004

    • Author(s)
      T.Yano, K.Yoshimoto et al.
    • Journal Title

      Neurochem Res 29

      Pages: 283-293

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of ethanol on the induction of Uncoupling protein-1 (UCP1) mRNA in the mouse brown adipose tissue.2004

    • Author(s)
      K.Yoshimoto et al.
    • Journal Title

      Tohoku J Exp Med 204

      Pages: 45-51

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of bofu-tsusho-san, an oriental herbal medicine, in obese Japanese women with impaired glucose tolerance.2004

    • Author(s)
      C.Hioki, K.Yoshimoto et al.
    • Journal Title

      Clin Exp Pharmacol Physiol 31

      Pages: 614-619

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Alterations in the function of cerebral dopaminergic and serotonergic systems following electroacupuncture and moxibution applications : Possible correlates with their antistress and psychosomatic actions.2004

    • Author(s)
      T.Yano, B.Kato, F.Fukuda, H.Shinbara, K.Yoshimoto, A.Ozaki, K.Kuriyama
    • Journal Title

      Neurochem.Res. 29(1)

      Pages: 283-293

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of ethanol on the induction of uncoupling protein-1(UCP1) mRNA in the mouse brown adipose tissue.2004

    • Author(s)
      K.Yoshimoto, M.Yasuhara, S.Komura, Y.Misumi, Y.Uchiyama, A.Kogure, C.Hioki, Y.Wakabayashi, Y.Satomi, A.Nishimura, F.Fukuda, M.Hori, C.Yokoyama, T.Yoshida.
    • Journal Title

      Tohoku J Exp Med 204

      Pages: 45-51

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Efficacy of bofu-tsusho-san, an oriental herval medicine, in obese Japanese women with impaired glucose tolerance.2004

    • Author(s)
      C.Hioki, K.Yoshimoto, T.Yoshida
    • Journal Title

      Clin Exp Pharmacol Physiol 31(9)

      Pages: 614-619

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The age of drinking onset and housing condition influences rat alcohol preference...2003

    • Author(s)
      K.Yoshimoto et al.
    • Journal Title

      Jpn J Alcohol and Drug Dependence 38

      Pages: 335-340

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pharmacological studies of alcohol susceptibility and brain monoamine function in SHRSP and SHRSR.2003

    • Author(s)
      K.Yoshimoto et al.
    • Journal Title

      Tohoku J Exp Med 201

      Pages: 11-22

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The age of drinking onset and housing condition influences rat alcohol drinking behavior.2003

    • Author(s)
      K.Yoshimoto, S.Komura, M.Yasuhara
    • Journal Title

      Jpn.J.Alcohol and Drug Dependence 38(4)

      Pages: 335-340

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Pharmacological studies of alcohol susceptibility and Brain monoamine function in stroke-prone spontaneously hypertensive rats (SHRSP) and stroke-resistant spontaneously hypertensive rats (SHRSR).2003

    • Author(s)
      K.Yoshimoto, S.Komura, H.Hattori, Y.Yamori, M.Yasuhara
    • Journal Title

      Tohoku J Exp Med 201

      Pages: 11-22

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2006-07-11  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi